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Effect of antisense peptide binding on the dimerization of human cystatin C - gel electrophoresis and molecular modeling studies

Stachowiak, K; Rodziewicz-Motowidlo, S; Sosnowska, R; Kasprzykowski, F; Lankiewicz, L; Grubb, Anders LU and Grzonka, Z (2004) In Acta Biochimica Polonica 51(1). p.153-160
Abstract
Human cystatin C (HCC) shows a tendency to dimerize. This process is particularly easy in the case of the L68Q HCC mutant and might lead to formation of amyloid deposits in brain arteries of young adults. Our purpose was to find ligands of monomeric HCC that can prevent its dimerization. Eleven antisense peptide ligands of monomeric HCC were designed and synthesized. The influence of these ligands on HCC dimerization was studied using gel electrophoresis and molecular modeling methods. The results suggest that all the designed peptides interact with monomeric HCC facilitating its dimerization rather than preventing it.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
dimerization, human cystatin C, antisense peptides, molecular dynamics
in
Acta Biochimica Polonica
volume
51
issue
1
pages
153 - 160
publisher
Panstwowe Wydawnictwo Naukowe
external identifiers
  • wos:000220612100016
  • scopus:2342454972
ISSN
0001-527X
language
English
LU publication?
yes
id
e631a451-d57d-4609-8c83-9ffb204a03ed (old id 281976)
alternative location
http://www.actabp.pl/html/1_2004/153.html
date added to LUP
2007-11-02 08:48:22
date last changed
2017-01-01 07:13:15
@article{e631a451-d57d-4609-8c83-9ffb204a03ed,
  abstract     = {Human cystatin C (HCC) shows a tendency to dimerize. This process is particularly easy in the case of the L68Q HCC mutant and might lead to formation of amyloid deposits in brain arteries of young adults. Our purpose was to find ligands of monomeric HCC that can prevent its dimerization. Eleven antisense peptide ligands of monomeric HCC were designed and synthesized. The influence of these ligands on HCC dimerization was studied using gel electrophoresis and molecular modeling methods. The results suggest that all the designed peptides interact with monomeric HCC facilitating its dimerization rather than preventing it.},
  author       = {Stachowiak, K and Rodziewicz-Motowidlo, S and Sosnowska, R and Kasprzykowski, F and Lankiewicz, L and Grubb, Anders and Grzonka, Z},
  issn         = {0001-527X},
  keyword      = {dimerization,human cystatin C,antisense peptides,molecular dynamics},
  language     = {eng},
  number       = {1},
  pages        = {153--160},
  publisher    = {Panstwowe Wydawnictwo Naukowe},
  series       = {Acta Biochimica Polonica},
  title        = {Effect of antisense peptide binding on the dimerization of human cystatin C - gel electrophoresis and molecular modeling studies},
  volume       = {51},
  year         = {2004},
}