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Composition and structure of mixed phospholipid supported bilayers formed by POPC and DPPC

Akesson, Anna; Lind, Tania; Ehrlich, Nicky; Stamou, Dimitrios; Wacklin, Hanna LU and Cardenas, Marite (2012) In Soft Matter 8(20). p.5658-5665
Abstract
In this paper we present a systematic study of the morphology and composition of supported lipid bilayers (SLBs) formed by vesicle fusion using a wide variety of surface sensitive techniques that give information about the lateral as well as vertical structure and bilayer fluidity. SLBs of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) mixtures at five different bulk vesicle compositions were formed in such a way that the phase separation boundaries were crossed. For all compositions studied, the SLBs were systematically enriched with POPC compared to the nominal vesicle composition. Nevertheless, gel-fluid domain coexistence was observed for SLB compositions in which phase... (More)
In this paper we present a systematic study of the morphology and composition of supported lipid bilayers (SLBs) formed by vesicle fusion using a wide variety of surface sensitive techniques that give information about the lateral as well as vertical structure and bilayer fluidity. SLBs of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) mixtures at five different bulk vesicle compositions were formed in such a way that the phase separation boundaries were crossed. For all compositions studied, the SLBs were systematically enriched with POPC compared to the nominal vesicle composition. Nevertheless, gel-fluid domain coexistence was observed for SLB compositions in which phase separation was expected based on the bulk phase diagram. The probable causes for the compositional difference in the SLBs are discussed in terms of the phase behaviour of the mixture and its effect on the membrane formation process by vesicle fusion. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
8
issue
20
pages
5658 - 5665
publisher
Royal Society of Chemistry
external identifiers
  • wos:000303998700030
  • scopus:84861122853
ISSN
1744-6848
DOI
10.1039/c2sm00013j
language
English
LU publication?
yes
id
b6d3fb90-997b-4683-a603-b8f898448e67 (old id 2826379)
date added to LUP
2012-06-20 15:08:15
date last changed
2017-11-12 03:47:21
@article{b6d3fb90-997b-4683-a603-b8f898448e67,
  abstract     = {In this paper we present a systematic study of the morphology and composition of supported lipid bilayers (SLBs) formed by vesicle fusion using a wide variety of surface sensitive techniques that give information about the lateral as well as vertical structure and bilayer fluidity. SLBs of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) mixtures at five different bulk vesicle compositions were formed in such a way that the phase separation boundaries were crossed. For all compositions studied, the SLBs were systematically enriched with POPC compared to the nominal vesicle composition. Nevertheless, gel-fluid domain coexistence was observed for SLB compositions in which phase separation was expected based on the bulk phase diagram. The probable causes for the compositional difference in the SLBs are discussed in terms of the phase behaviour of the mixture and its effect on the membrane formation process by vesicle fusion.},
  author       = {Akesson, Anna and Lind, Tania and Ehrlich, Nicky and Stamou, Dimitrios and Wacklin, Hanna and Cardenas, Marite},
  issn         = {1744-6848},
  language     = {eng},
  number       = {20},
  pages        = {5658--5665},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {Composition and structure of mixed phospholipid supported bilayers formed by POPC and DPPC},
  url          = {http://dx.doi.org/10.1039/c2sm00013j},
  volume       = {8},
  year         = {2012},
}