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Lipid-Based Passivation in Nanofluidics

Persson, Fredrik; Fritzsche, Joachim; Mir, Kalim U.; Modesti, Mauro; Westerlund, Fredrik and Tegenfeldt, Jonas LU (2012) In Nano Letters 12(5). p.2260-2265
Abstract
Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by... (More)
Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein-DNA interactions with high spatial and temporal resolution. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Nanofluidics, passivation, antifouling, lipid bilayer, protein-DNA, interactions, single molecules
in
Nano Letters
volume
12
issue
5
pages
2260 - 2265
publisher
The American Chemical Society
external identifiers
  • wos:000303696400015
  • scopus:84861014879
ISSN
1530-6992
DOI
10.1021/nl204535h
language
English
LU publication?
yes
id
6bd025be-cdd8-4c72-9d04-6c4350b7407f (old id 2826790)
date added to LUP
2012-06-20 13:34:46
date last changed
2017-09-24 04:09:36
@article{6bd025be-cdd8-4c72-9d04-6c4350b7407f,
  abstract     = {Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein-DNA interactions with high spatial and temporal resolution.},
  author       = {Persson, Fredrik and Fritzsche, Joachim and Mir, Kalim U. and Modesti, Mauro and Westerlund, Fredrik and Tegenfeldt, Jonas},
  issn         = {1530-6992},
  keyword      = {Nanofluidics,passivation,antifouling,lipid bilayer,protein-DNA,interactions,single molecules},
  language     = {eng},
  number       = {5},
  pages        = {2260--2265},
  publisher    = {The American Chemical Society},
  series       = {Nano Letters},
  title        = {Lipid-Based Passivation in Nanofluidics},
  url          = {http://dx.doi.org/10.1021/nl204535h},
  volume       = {12},
  year         = {2012},
}