Advanced

Biosensors and Chromatographic Supports Based on New Combinations of Conductive Materials

Khayyami, Masoud (1996)
Abstract
Several approaches towards the optimization of amperometric biosensors using a soluble mediator in combination with reticulated vitreous carbon (RVC) were studied. RVC material was used as the working electrode with biomolecules (enzyme/co-enzyme) immobilised on its surface and intergrated with a FIA system. By using the soluble electron transfer mediator Meldola Blue the oxidation potentials for the electrochemical reactions were reduced, decreasing the interference by compounds that may be oxidised at higher potentials. Electrodes for the determination of glucose, lactate, acetylthiocholine and paraoxon were prepared. The influences of various parameters on the electrode sensitivity were investigated. In a glucose sensor, ferrocene was... (More)
Several approaches towards the optimization of amperometric biosensors using a soluble mediator in combination with reticulated vitreous carbon (RVC) were studied. RVC material was used as the working electrode with biomolecules (enzyme/co-enzyme) immobilised on its surface and intergrated with a FIA system. By using the soluble electron transfer mediator Meldola Blue the oxidation potentials for the electrochemical reactions were reduced, decreasing the interference by compounds that may be oxidised at higher potentials. Electrodes for the determination of glucose, lactate, acetylthiocholine and paraoxon were prepared. The influences of various parameters on the electrode sensitivity were investigated. In a glucose sensor, ferrocene was used as electron transfer mediator together with a conducting polymer (polypyrrole) for electron transport between the enzyme (glucose oxidase) and the electrode surface (RVC). The glucose oxidation was monitored by calorimetry while the system was electrochemically regenerated. The metabolic activity of yeast cells could be monitord by a DC resistometric method using the conducting polymer polypyrrole for immobilisation of the cells. The cobination of superporous agarose gel with electroactive material was used for construction of a chromatographic support material that was both electrically conducting and electrochemically active. A specially designed chromatography column containing this material was connected to a potentiostat and, by changing the applied potential, reduction or oxidation of the support material could be accomplished which influenced the chromatographic properties substantially. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Prof. Satoh, Ikuo, Department Applied Chemistry, Kanagawa Institute of Technology, Atusugi-shi, Kanagawa-ken, Japan
publishing date
type
Thesis
publication status
published
subject
keywords
Biochemistry, Superporous agarose, Chromatography, Polypyrrole, conducting polymer, Amperometric biosensor, Reticulated Vitreous Carbon, RVC, Metabolism, Biokemi, metabolism, Biotechnology, Bioteknik
pages
46 pages
publisher
TMS CHEM AB, Ideon, S-223 70 Lund, Sweden
defense location
Chemical centre, hall C, Sölvegatan 39, Lund, Sweden
defense date
1996-05-23 10:15
external identifiers
  • other:LUTKDH/TBK-1040/1-128/1996
ISBN
91-628-2079-6
language
English
LU publication?
no
id
7b2299a7-4161-4d20-b699-5185393a032b (old id 28442)
date added to LUP
2007-06-12 14:29:07
date last changed
2016-09-19 08:45:06
@phdthesis{7b2299a7-4161-4d20-b699-5185393a032b,
  abstract     = {Several approaches towards the optimization of amperometric biosensors using a soluble mediator in combination with reticulated vitreous carbon (RVC) were studied. RVC material was used as the working electrode with biomolecules (enzyme/co-enzyme) immobilised on its surface and intergrated with a FIA system. By using the soluble electron transfer mediator Meldola Blue the oxidation potentials for the electrochemical reactions were reduced, decreasing the interference by compounds that may be oxidised at higher potentials. Electrodes for the determination of glucose, lactate, acetylthiocholine and paraoxon were prepared. The influences of various parameters on the electrode sensitivity were investigated. In a glucose sensor, ferrocene was used as electron transfer mediator together with a conducting polymer (polypyrrole) for electron transport between the enzyme (glucose oxidase) and the electrode surface (RVC). The glucose oxidation was monitored by calorimetry while the system was electrochemically regenerated. The metabolic activity of yeast cells could be monitord by a DC resistometric method using the conducting polymer polypyrrole for immobilisation of the cells. The cobination of superporous agarose gel with electroactive material was used for construction of a chromatographic support material that was both electrically conducting and electrochemically active. A specially designed chromatography column containing this material was connected to a potentiostat and, by changing the applied potential, reduction or oxidation of the support material could be accomplished which influenced the chromatographic properties substantially.},
  author       = {Khayyami, Masoud},
  isbn         = {91-628-2079-6},
  keyword      = {Biochemistry,Superporous agarose,Chromatography,Polypyrrole,conducting polymer,Amperometric biosensor,Reticulated Vitreous Carbon,RVC,Metabolism,Biokemi,metabolism,Biotechnology,Bioteknik},
  language     = {eng},
  pages        = {46},
  publisher    = {TMS CHEM AB, Ideon, S-223 70 Lund, Sweden},
  title        = {Biosensors and Chromatographic Supports Based on New Combinations of Conductive Materials},
  year         = {1996},
}