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Gastric Bypass Surgery in Mice Does Not Change Islet Size nor Composition

Vangoitsenhoven, Roman ; Maris, Michael ; da Cunha, Joao Paulo LU ; Lannoo, Matthias ; Overbergh, Lut ; Mathieu, Chantal and Van der Schueren, Bart (2015) 75th Scientific Sessions of the American Diabetes Association In Diabetes 64(Suppl 1). p.550-550
Abstract
Gastric bypass surgery improves type 2 diabetes, but the weight loss independent mechanisms remain elusive. We set out to unravel a potential
benefi cial role of gastric bypass surgery on islets of Langerhans.
Eight week old male C57Bl/6 mice were fed a high fat diet (60% kcal fat)
for 14 weeks and subsequently subjected to Roux-en-Y gastric bypass (RYGB;
n=16). Weight matched (WMS; n=13) and pair fed sham operated mice (PFS;
n=14) were used as controls. 8 weeks post-surgery, mice were phenotyped
by intraperitoneal glucose tolerance test (IPGTT) and indirect calorimetry.
Pancreatic tissue was analyzed by immunohistochemistry.
Eight weeks after surgery, RYGB and WMS mice showed abundant weight
loss,... (More)
Gastric bypass surgery improves type 2 diabetes, but the weight loss independent mechanisms remain elusive. We set out to unravel a potential
benefi cial role of gastric bypass surgery on islets of Langerhans.
Eight week old male C57Bl/6 mice were fed a high fat diet (60% kcal fat)
for 14 weeks and subsequently subjected to Roux-en-Y gastric bypass (RYGB;
n=16). Weight matched (WMS; n=13) and pair fed sham operated mice (PFS;
n=14) were used as controls. 8 weeks post-surgery, mice were phenotyped
by intraperitoneal glucose tolerance test (IPGTT) and indirect calorimetry.
Pancreatic tissue was analyzed by immunohistochemistry.
Eight weeks after surgery, RYGB and WMS mice showed abundant weight
loss, decreased fasting glucose and insulin levels, as well as improved glucose tolerance as compared to obese PFS mice (Table 1). Additionally, energy
expenditure was higher in RYGB (2022 ± 145 kJ/day) as compared to WMS
(1756 ± 284 kJ/day, P < 0.05) and PFS (1863 ± 195 kJ/day, P 0.11). Pancreatic
islet size, as well as insulin, glucagon and somatostatin positive area per
islet were similar between all groups.
In summary, RYGB surgery improves glucose homeostasis and increases
energy expenditure, but has no effect on size or cell composition of islets of
Langerhans. As improved beta cell function has been reported after RYGB,
RNA-Seq analysis of isolated islets is currently ongoing in order to reveal the
underlying molecular mechanisms. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Diabetes
volume
64
issue
Suppl 1
pages
550 - 550
publisher
American Diabetes Association Inc.
conference name
75th Scientific Sessions of the American Diabetes Association
conference location
Boston, United States
conference dates
2015-06-05 - 2015-06-09
ISSN
1939-327X
DOI
10.2337/db15-1929-2253
language
English
LU publication?
no
id
2849c3ab-51b0-48c6-b831-bd06dd356f5a
date added to LUP
2020-01-30 15:25:07
date last changed
2020-02-04 13:24:25
@misc{2849c3ab-51b0-48c6-b831-bd06dd356f5a,
  abstract     = {Gastric bypass surgery improves type 2 diabetes, but the weight loss independent mechanisms remain elusive. We set out to unravel a potential<br>
benefi cial role of gastric bypass surgery on islets of Langerhans.<br>
Eight week old male C57Bl/6 mice were fed a high fat diet (60% kcal fat)<br>
for 14 weeks and subsequently subjected to Roux-en-Y gastric bypass (RYGB;<br>
n=16). Weight matched (WMS; n=13) and pair fed sham operated mice (PFS;<br>
n=14) were used as controls. 8 weeks post-surgery, mice were phenotyped<br>
by intraperitoneal glucose tolerance test (IPGTT) and indirect calorimetry.<br>
Pancreatic tissue was analyzed by immunohistochemistry.<br>
Eight weeks after surgery, RYGB and WMS mice showed abundant weight<br>
loss, decreased fasting glucose and insulin levels, as well as improved glucose tolerance as compared to obese PFS mice (Table 1). Additionally, energy<br>
expenditure was higher in RYGB (2022 ± 145 kJ/day) as compared to WMS<br>
(1756 ± 284 kJ/day, P &lt; 0.05) and PFS (1863 ± 195 kJ/day, P 0.11). Pancreatic<br>
islet size, as well as insulin, glucagon and somatostatin positive area per<br>
islet were similar between all groups.<br>
In summary, RYGB surgery improves glucose homeostasis and increases<br>
energy expenditure, but has no effect on size or cell composition of islets of<br>
Langerhans. As improved beta cell function has been reported after RYGB,<br>
RNA-Seq analysis of isolated islets is currently ongoing in order to reveal the<br>
underlying molecular mechanisms.},
  author       = {Vangoitsenhoven, Roman and Maris, Michael and da Cunha, Joao Paulo and Lannoo, Matthias and Overbergh, Lut and Mathieu, Chantal and Van der Schueren, Bart},
  issn         = {1939-327X},
  language     = {eng},
  note         = {Conference Abstract},
  number       = {Suppl 1},
  pages        = {550--550},
  publisher    = {American Diabetes Association Inc.},
  series       = {Diabetes},
  title        = {Gastric Bypass Surgery in Mice Does Not Change Islet Size nor Composition},
  url          = {http://dx.doi.org/10.2337/db15-1929-2253},
  doi          = {10.2337/db15-1929-2253},
  volume       = {64},
  year         = {2015},
}