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Analysis of halogenated fatty acids in fish lipids by gas chromatography with electrolytic conductivity detection and mass spectrometry

Mu, Huiling (1996)
Abstract
Organochlorine compounds in fish lipids were characterized by studying the partitioning of chlorinated species into acidic and neutral fractions before and after hydrolysis of the lipids, and after transesterification to fatty acid methyl esters (FAMEs). Chlorinated fatty acids (Cl-FAs) were found to be the major organochlorine components in the fish lipids. Approximately 90% of the extractable organically bound chlorine (EOCl) in the lipids of eel, caught in a fjord polluted by pulp mill waste waters, could be accounted for by chlorine in Cl-FAs, by using gas chromatography (GC) with electrolytic conductivity detection (ELCD).



An enrichment method for chlorinated FAMEs was developed, in order to study organochlorine... (More)
Organochlorine compounds in fish lipids were characterized by studying the partitioning of chlorinated species into acidic and neutral fractions before and after hydrolysis of the lipids, and after transesterification to fatty acid methyl esters (FAMEs). Chlorinated fatty acids (Cl-FAs) were found to be the major organochlorine components in the fish lipids. Approximately 90% of the extractable organically bound chlorine (EOCl) in the lipids of eel, caught in a fjord polluted by pulp mill waste waters, could be accounted for by chlorine in Cl-FAs, by using gas chromatography (GC) with electrolytic conductivity detection (ELCD).



An enrichment method for chlorinated FAMEs was developed, in order to study organochlorine compounds in lipids of fish containing low concentrations of EOCl. The enrichment was achieved by the removal of unsubstituted, straight-chain FAMEs and polyunsaturated FAMEs as their urea complexes and silver ion complexes, respectively. Following the enrichment, Cl-FAs could be detected by GC/ELCD in the lipids of several other fish species. Dichloroalkanoic, dichloroalkenoic, and tetrachloroalkanoic acids in the eel lipids were identified as the corresponding methyl esters by using mass spectrometry (MS). High resolution MS was used for confirmation.



Halogenated fatty acids (X-FAs) also contributed significantly to the organohalogen compounds in muscle lipids and roe lipids of sockeye salmon from Alaska. X-FAs bound in triacylglycerols from salmon muscle were catabolized and transferred to roe to approximately the same extent as normal fatty acids during a spawning migration. It was also found that Cl-FAs were transferred from salmon to another fish species. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Dr. Larsson, Lennart
publishing date
type
Thesis
publication status
published
subject
keywords
metabolism., identification, methanolysis, enzymatic hydrolysis, chlorinated fatty acids, organohalogen compounds, mass spectrometry, Gas chromatography, electrolytic conductivity detection, Analytical chemistry, Analytisk kemi
pages
29 pages
publisher
Analytical Chemistry, Lund University
defense location
Center for Chemistry and Chemical Engineering, Sölvegatan 39, lecture hall D
defense date
1996-06-13 13:15
external identifiers
  • Other:LUTKDH/(TKAK-1015)/1-136 (1996)
ISBN
91-628-2064-8
language
English
LU publication?
no
id
3f6f95cf-5a82-4c14-a158-ed2d5b8b9a1e (old id 28546)
date added to LUP
2007-06-12 14:51:51
date last changed
2016-09-19 08:45:12
@phdthesis{3f6f95cf-5a82-4c14-a158-ed2d5b8b9a1e,
  abstract     = {Organochlorine compounds in fish lipids were characterized by studying the partitioning of chlorinated species into acidic and neutral fractions before and after hydrolysis of the lipids, and after transesterification to fatty acid methyl esters (FAMEs). Chlorinated fatty acids (Cl-FAs) were found to be the major organochlorine components in the fish lipids. Approximately 90% of the extractable organically bound chlorine (EOCl) in the lipids of eel, caught in a fjord polluted by pulp mill waste waters, could be accounted for by chlorine in Cl-FAs, by using gas chromatography (GC) with electrolytic conductivity detection (ELCD).<br/><br>
<br/><br>
An enrichment method for chlorinated FAMEs was developed, in order to study organochlorine compounds in lipids of fish containing low concentrations of EOCl. The enrichment was achieved by the removal of unsubstituted, straight-chain FAMEs and polyunsaturated FAMEs as their urea complexes and silver ion complexes, respectively. Following the enrichment, Cl-FAs could be detected by GC/ELCD in the lipids of several other fish species. Dichloroalkanoic, dichloroalkenoic, and tetrachloroalkanoic acids in the eel lipids were identified as the corresponding methyl esters by using mass spectrometry (MS). High resolution MS was used for confirmation.<br/><br>
<br/><br>
Halogenated fatty acids (X-FAs) also contributed significantly to the organohalogen compounds in muscle lipids and roe lipids of sockeye salmon from Alaska. X-FAs bound in triacylglycerols from salmon muscle were catabolized and transferred to roe to approximately the same extent as normal fatty acids during a spawning migration. It was also found that Cl-FAs were transferred from salmon to another fish species.},
  author       = {Mu, Huiling},
  isbn         = {91-628-2064-8},
  keyword      = {metabolism.,identification,methanolysis,enzymatic hydrolysis,chlorinated fatty acids,organohalogen compounds,mass spectrometry,Gas chromatography,electrolytic conductivity detection,Analytical chemistry,Analytisk kemi},
  language     = {eng},
  pages        = {29},
  publisher    = {Analytical Chemistry, Lund University},
  title        = {Analysis of halogenated fatty acids in fish lipids by gas chromatography with electrolytic conductivity detection and mass spectrometry},
  year         = {1996},
}