FGF-2-responsive neural stem cell proliferation requires CCg, a novel autocrine/paracrine cofactor
Taupin, Philippe ; Ray, Jasodhara ; Fischer, Wolfgang H. ; Suhr, Steven T. ; Hakansson, Katarina ; Grubb, Anders LU
and Gage, Fred H.
(2000)
In Neuron
28(2).
p.97-385
- Abstract
We have purified and characterized a factor, from the conditioned medium of neural stem cell cultures, which is required for fibroblast growth factor 2's (FGF-2) mitogenic activity on neural stem cells. This autocrine/paracrine cofactor is a glycosylated form of cystatin C (CCg), whose N-glycosylation is required for its activity. We further demonstrated that, both in vitro and in vivo, neural stem cells undergoing cell division are immunopositive for cystatin C. Finally, we showed in vivo functional activity of CCg by demonstrating that the combined delivery of FGF-2 and CCg to the adult dentate gyrus stimulated neurogenesis. We propose that the process of neurogenesis is controlled by the cooperation between trophic factors and... (More)
We have purified and characterized a factor, from the conditioned medium of neural stem cell cultures, which is required for fibroblast growth factor 2's (FGF-2) mitogenic activity on neural stem cells. This autocrine/paracrine cofactor is a glycosylated form of cystatin C (CCg), whose N-glycosylation is required for its activity. We further demonstrated that, both in vitro and in vivo, neural stem cells undergoing cell division are immunopositive for cystatin C. Finally, we showed in vivo functional activity of CCg by demonstrating that the combined delivery of FGF-2 and CCg to the adult dentate gyrus stimulated neurogenesis. We propose that the process of neurogenesis is controlled by the cooperation between trophic factors and autocrine/paracrine cofactors, of which CCg is a prototype.
(Less)
- author
- Taupin, Philippe
; Ray, Jasodhara
; Fischer, Wolfgang H.
; Suhr, Steven T.
; Hakansson, Katarina
; Grubb, Anders
LU
and Gage, Fred H.
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Autocrine Communication/drug effects, Cell Division/drug effects, Cells, Cultured, Culture Media, Conditioned/chemistry, Cystatin C, Cystatins/chemistry, Dentate Gyrus/cytology, Fibroblast Growth Factor 2/genetics, Glycosylation, Hippocampus/cytology, Molecular Sequence Data, Molecular Weight, Neurons/cytology, Paracrine Communication/drug effects, Protein Processing, Post-Translational, Rats, Sequence Analysis, Protein, Stem Cell Transplantation, Stem Cells/cytology
- in
- Neuron
- volume
- 28
- issue
- 2
- pages
- 97 - 385
- publisher
- Cell Press
- external identifiers
-
- scopus:0033635021
- pmid:11144350
- ISSN
- 0896-6273
- DOI
- 10.1016/s0896-6273(00)00119-7
- language
- English
- LU publication?
- no
- id
- 28590c8b-274c-48cd-92dd-0ae5c2b8d612
- date added to LUP
- 2021-11-02 14:40:19
- date last changed
- 2024-04-20 15:36:49
@article{28590c8b-274c-48cd-92dd-0ae5c2b8d612,
abstract = {{<p>We have purified and characterized a factor, from the conditioned medium of neural stem cell cultures, which is required for fibroblast growth factor 2's (FGF-2) mitogenic activity on neural stem cells. This autocrine/paracrine cofactor is a glycosylated form of cystatin C (CCg), whose N-glycosylation is required for its activity. We further demonstrated that, both in vitro and in vivo, neural stem cells undergoing cell division are immunopositive for cystatin C. Finally, we showed in vivo functional activity of CCg by demonstrating that the combined delivery of FGF-2 and CCg to the adult dentate gyrus stimulated neurogenesis. We propose that the process of neurogenesis is controlled by the cooperation between trophic factors and autocrine/paracrine cofactors, of which CCg is a prototype.</p>}},
author = {{Taupin, Philippe and Ray, Jasodhara and Fischer, Wolfgang H. and Suhr, Steven T. and Hakansson, Katarina and Grubb, Anders and Gage, Fred H.}},
issn = {{0896-6273}},
keywords = {{Animals; Autocrine Communication/drug effects; Cell Division/drug effects; Cells, Cultured; Culture Media, Conditioned/chemistry; Cystatin C; Cystatins/chemistry; Dentate Gyrus/cytology; Fibroblast Growth Factor 2/genetics; Glycosylation; Hippocampus/cytology; Molecular Sequence Data; Molecular Weight; Neurons/cytology; Paracrine Communication/drug effects; Protein Processing, Post-Translational; Rats; Sequence Analysis, Protein; Stem Cell Transplantation; Stem Cells/cytology}},
language = {{eng}},
number = {{2}},
pages = {{97--385}},
publisher = {{Cell Press}},
series = {{Neuron}},
title = {{FGF-2-responsive neural stem cell proliferation requires CCg, a novel autocrine/paracrine cofactor}},
url = {{http://dx.doi.org/10.1016/s0896-6273(00)00119-7}},
doi = {{10.1016/s0896-6273(00)00119-7}},
volume = {{28}},
year = {{2000}},
}