The role of polyamines in cell cycle progression
(1996)- Abstract
- In an attempt to shed light on the role of the polyamines, putrescine, spermidine, and spermine in cell cycle progression, polyamine biosynthesis has been analyzed during the cell cycle of Chinese hamster ovary cells, and cell cycle kinetics have been investigated in cells treated with polyamine biosynthesis inhibitors. Cells were synchronized with the mitotic detachment technique, and at various times after seeding, they were sampled for analysis of ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) activities, as well as their mRNA levels and polyamine contents. The results imply that polyamine biosynthesis was regulated at the levels of transcription, translation, and post-translation. The effect of... (More)
- In an attempt to shed light on the role of the polyamines, putrescine, spermidine, and spermine in cell cycle progression, polyamine biosynthesis has been analyzed during the cell cycle of Chinese hamster ovary cells, and cell cycle kinetics have been investigated in cells treated with polyamine biosynthesis inhibitors. Cells were synchronized with the mitotic detachment technique, and at various times after seeding, they were sampled for analysis of ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) activities, as well as their mRNA levels and polyamine contents. The results imply that polyamine biosynthesis was regulated at the levels of transcription, translation, and post-translation. The effect of polyamine depletion on cell cycle kinetics was studied with a bromodeoxyuridine - flow cytometry technique. Polyamine depletion was achieved by treating the cells with either 2-difluoromethylornithine (DFMO), an inhibitor of ODC, or 4-amidinoindan-1-one 21-amidinohydrazone (CGP 48664), an inhibitor of AdoMetDC. DFMO treatment resulted in depletion of the putrescine and spermidine pools, while CGP 48664 treatment resulted in depletion of the spermidine and spermine pools. Treatment with either of the inhibitors resulted in a reduction in the rate of progression through S phase within one cell cycle. This was not caused by an affect on the G1/S transition. The results indicate that polyamine depletion reduced the rate of DNA elongation without affecting the initiation of DNA replication. CGP 48664 treatment resulted in a more pronounced inhibition of S phase progression than did DFMO treatment, indicating that spermine may have a more pronounced role in DNA replication than the other polyamines. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/28600
- author
- Fredlund, Jan O
- supervisor
- opponent
-
- Bruce Stanley, Bruce Stanley
- publishing date
- 1996
- type
- Thesis
- publication status
- published
- subject
- keywords
- Animal physiology, Djurfysiologi
- pages
- 49 pages
- defense location
- Lecture hall at the Dpt Animal. Physiol.
- defense date
- 1996-09-13 10:15:00
- external identifiers
-
- other:ISRN: LUNBDS/NBZF-1044-SE
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Animal Physiology (Closed 2011) (011011000)
- id
- b699b94f-c0a4-4e21-8067-f87301187a55 (old id 28600)
- date added to LUP
- 2016-04-04 14:30:25
- date last changed
- 2018-11-21 21:20:42
@phdthesis{b699b94f-c0a4-4e21-8067-f87301187a55, abstract = {{In an attempt to shed light on the role of the polyamines, putrescine, spermidine, and spermine in cell cycle progression, polyamine biosynthesis has been analyzed during the cell cycle of Chinese hamster ovary cells, and cell cycle kinetics have been investigated in cells treated with polyamine biosynthesis inhibitors. Cells were synchronized with the mitotic detachment technique, and at various times after seeding, they were sampled for analysis of ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) activities, as well as their mRNA levels and polyamine contents. The results imply that polyamine biosynthesis was regulated at the levels of transcription, translation, and post-translation. The effect of polyamine depletion on cell cycle kinetics was studied with a bromodeoxyuridine - flow cytometry technique. Polyamine depletion was achieved by treating the cells with either 2-difluoromethylornithine (DFMO), an inhibitor of ODC, or 4-amidinoindan-1-one 21-amidinohydrazone (CGP 48664), an inhibitor of AdoMetDC. DFMO treatment resulted in depletion of the putrescine and spermidine pools, while CGP 48664 treatment resulted in depletion of the spermidine and spermine pools. Treatment with either of the inhibitors resulted in a reduction in the rate of progression through S phase within one cell cycle. This was not caused by an affect on the G1/S transition. The results indicate that polyamine depletion reduced the rate of DNA elongation without affecting the initiation of DNA replication. CGP 48664 treatment resulted in a more pronounced inhibition of S phase progression than did DFMO treatment, indicating that spermine may have a more pronounced role in DNA replication than the other polyamines.}}, author = {{Fredlund, Jan O}}, keywords = {{Animal physiology; Djurfysiologi}}, language = {{eng}}, title = {{The role of polyamines in cell cycle progression}}, year = {{1996}}, }