Germinal center B cells constitute a predominant physiological source of IL-4: Implication for Th2 development in vivo
(2002) In Journal of Immunology 168(7). p.3165-3172- Abstract
- Protective immunity depends upon the capability of the immune system to properly adapt the response to the nature of an infectious agent. CD4+ Th cells are implicated in this orchestration by secreting a polarized pattern of cytokines. Although Th2 development in animal models and in human cells in vitro to a large extent depends on IL-4, the nature of the cells that provide the initial IL-4 in vivo is still elusive. In this report, we describe the anatomical localization as well as the identity of IL-4-producing cells in human tonsil, a representative secondary lymphoid organ. We demonstrate that IL-4 production is a normal and intrinsic feature of germinal center (GC) B cells. We also show that expression of IL-4 is highly confined to... (More)
- Protective immunity depends upon the capability of the immune system to properly adapt the response to the nature of an infectious agent. CD4+ Th cells are implicated in this orchestration by secreting a polarized pattern of cytokines. Although Th2 development in animal models and in human cells in vitro to a large extent depends on IL-4, the nature of the cells that provide the initial IL-4 in vivo is still elusive. In this report, we describe the anatomical localization as well as the identity of IL-4-producing cells in human tonsil, a representative secondary lymphoid organ. We demonstrate that IL-4 production is a normal and intrinsic feature of germinal center (GC) B cells. We also show that expression of IL-4 is highly confined to the GCs, in which the B cells constitute the prevalent cellular source. Furthermore, immunofluorescence analysis of colon mucosa reveals a strikingly similar pattern of IL-4-expressing cells compared with tonsils, demonstrating that IL-4 production from GC B cells is not a unique feature of the upper respiratory tract. Our results show that GCs provide the most appropriate microenvironment for IL-4-dependent Th2 polarization in vivo and imply a critical role for GC B cells in this differentiation process. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/341839
- author
- Johansson-Lindbom, Bengt and Borrebaeck, Carl LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunology
- volume
- 168
- issue
- 7
- pages
- 3165 - 3172
- publisher
- American Association of Immunologists
- external identifiers
-
- pmid:11907068
- wos:000174566400008
- scopus:0036533540
- ISSN
- 1550-6606
- language
- English
- LU publication?
- yes
- id
- 2870a11b-92ee-42f0-8549-27f7c933f2e8 (old id 341839)
- date added to LUP
- 2016-04-01 16:47:57
- date last changed
- 2022-02-27 23:44:13
@article{2870a11b-92ee-42f0-8549-27f7c933f2e8,
abstract = {{Protective immunity depends upon the capability of the immune system to properly adapt the response to the nature of an infectious agent. CD4+ Th cells are implicated in this orchestration by secreting a polarized pattern of cytokines. Although Th2 development in animal models and in human cells in vitro to a large extent depends on IL-4, the nature of the cells that provide the initial IL-4 in vivo is still elusive. In this report, we describe the anatomical localization as well as the identity of IL-4-producing cells in human tonsil, a representative secondary lymphoid organ. We demonstrate that IL-4 production is a normal and intrinsic feature of germinal center (GC) B cells. We also show that expression of IL-4 is highly confined to the GCs, in which the B cells constitute the prevalent cellular source. Furthermore, immunofluorescence analysis of colon mucosa reveals a strikingly similar pattern of IL-4-expressing cells compared with tonsils, demonstrating that IL-4 production from GC B cells is not a unique feature of the upper respiratory tract. Our results show that GCs provide the most appropriate microenvironment for IL-4-dependent Th2 polarization in vivo and imply a critical role for GC B cells in this differentiation process.}},
author = {{Johansson-Lindbom, Bengt and Borrebaeck, Carl}},
issn = {{1550-6606}},
language = {{eng}},
number = {{7}},
pages = {{3165--3172}},
publisher = {{American Association of Immunologists}},
series = {{Journal of Immunology}},
title = {{Germinal center B cells constitute a predominant physiological source of IL-4: Implication for Th2 development in vivo}},
volume = {{168}},
year = {{2002}},
}