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Osteopontin is an adhesive factor for myeloma cells and is found in increased levels in plasma from patients with multiple myeloma

Standal, T; Hjorth-Hansen, H; Rasmussen, T; Dahl, IMS; Lenhoff, Stig LU ; Brenne, AT; Seidel, C; Baykov, V; Waage, A and Borset, M, et al. (2004) In Haematologica 89(2). p.174-182
Abstract
Background and Objectives. Osteopontin (OPN) is a non-collagenous matrix protein produced by various cells including osteoblasts, osteoclasts and several types of tumor cells. It is involved in a number of physiologic and pathologic events including adhesion, angiogenesis, apoptosis, inflammation, wound healing and tumor metastasis. We wanted to investigate the potential role of OPN in multiple myeloma. Design and Methods. Myeloma cells and stromal cells from myeloma patients were investigated as potential OPN-producers. Furthermore, OPN was tested in proliferation, migration and adhesion assays with myeloma cells. Serum and plasma OPN in myeloma patients were measured by enzyme-linked immunosorbent assay (ELISA). OPN levels were... (More)
Background and Objectives. Osteopontin (OPN) is a non-collagenous matrix protein produced by various cells including osteoblasts, osteoclasts and several types of tumor cells. It is involved in a number of physiologic and pathologic events including adhesion, angiogenesis, apoptosis, inflammation, wound healing and tumor metastasis. We wanted to investigate the potential role of OPN in multiple myeloma. Design and Methods. Myeloma cells and stromal cells from myeloma patients were investigated as potential OPN-producers. Furthermore, OPN was tested in proliferation, migration and adhesion assays with myeloma cells. Serum and plasma OPN in myeloma patients were measured by enzyme-linked immunosorbent assay (ELISA). OPN levels were correlated to disease variables at diagnosis and to disease outcome. Results. Myeloma cells produce OPN, and stromal cells from myeloma patients express higher levels of OPN than stromal cells from healthy controls. The myeloma cell lines ANBL-6 and INA-6 adhered to OPN. NOD/SCID mice inoculated with OPN-producing ANBL-6 cells had elevated levels of murine OPN in serum, whereas human OPN was not detectable. Plasma and serum levels of OPN were significantly higher in myeloma patients than in healthy individuals. Interpretation and Conclusions. Myeloma cell lines adhere to OPN, indicating that elevated stromal expression of OPN may be one of the factors responsible for the retention of myeloma cells in the bone marrow. The elevated plasma OPN levels in myeloma patients could be due to both production of OPN by the tumor cells and tumor-induced production of OPN by non-tumor cells. (Less)
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Contribution to journal
publication status
published
subject
keywords
adhesion, osteopontin, myeloma, stromal cells
in
Haematologica
volume
89
issue
2
pages
174 - 182
publisher
Ferrata Storti Foundation
external identifiers
  • pmid:15003892
  • wos:000189040000007
  • scopus:2942606661
ISSN
1592-8721
language
English
LU publication?
yes
id
653ae816-4a8c-4006-993a-de1d184e4130 (old id 287126)
alternative location
http://online.haematologica.org/backissues/medline.php?PMID=15003892
date added to LUP
2007-10-26 11:32:00
date last changed
2017-11-19 04:06:01
@article{653ae816-4a8c-4006-993a-de1d184e4130,
  abstract     = {Background and Objectives. Osteopontin (OPN) is a non-collagenous matrix protein produced by various cells including osteoblasts, osteoclasts and several types of tumor cells. It is involved in a number of physiologic and pathologic events including adhesion, angiogenesis, apoptosis, inflammation, wound healing and tumor metastasis. We wanted to investigate the potential role of OPN in multiple myeloma. Design and Methods. Myeloma cells and stromal cells from myeloma patients were investigated as potential OPN-producers. Furthermore, OPN was tested in proliferation, migration and adhesion assays with myeloma cells. Serum and plasma OPN in myeloma patients were measured by enzyme-linked immunosorbent assay (ELISA). OPN levels were correlated to disease variables at diagnosis and to disease outcome. Results. Myeloma cells produce OPN, and stromal cells from myeloma patients express higher levels of OPN than stromal cells from healthy controls. The myeloma cell lines ANBL-6 and INA-6 adhered to OPN. NOD/SCID mice inoculated with OPN-producing ANBL-6 cells had elevated levels of murine OPN in serum, whereas human OPN was not detectable. Plasma and serum levels of OPN were significantly higher in myeloma patients than in healthy individuals. Interpretation and Conclusions. Myeloma cell lines adhere to OPN, indicating that elevated stromal expression of OPN may be one of the factors responsible for the retention of myeloma cells in the bone marrow. The elevated plasma OPN levels in myeloma patients could be due to both production of OPN by the tumor cells and tumor-induced production of OPN by non-tumor cells.},
  author       = {Standal, T and Hjorth-Hansen, H and Rasmussen, T and Dahl, IMS and Lenhoff, Stig and Brenne, AT and Seidel, C and Baykov, V and Waage, A and Borset, M and Sundan, A and Hjertner, O},
  issn         = {1592-8721},
  keyword      = {adhesion,osteopontin,myeloma,stromal cells},
  language     = {eng},
  number       = {2},
  pages        = {174--182},
  publisher    = {Ferrata Storti Foundation},
  series       = {Haematologica},
  title        = {Osteopontin is an adhesive factor for myeloma cells and is found in increased levels in plasma from patients with multiple myeloma},
  volume       = {89},
  year         = {2004},
}