Capillary isoelectric focusing of proteins utilizing poly(vinylpyrrolodone)- and plexigla-coated columns
(2004) In Journal of Separation Science 27(1-2). p.124-128- Abstract
- Fused-silica capillaries chemically derivatized with silane/poly(vinylpyrrolidone) (PVP) or dynamically modified with plexiglas [poly(methyl methacrylate)] were prepared and evaluated with regard to column stability and separation performance for capillary isoelectric focusing of standard proteins. The PVP coating showed the better stability and was good for at least 100 runs while the plexiglas coating started to deteriorate after about 30 runs. The time spent for the plexiglas coating is about 40 minutes while the PVP coating requires two days. The migration time reproducibility was better with the PVP capillary (RSD 0.7-1.6%, n = 5) compared to the plexiglas-coated column (RSD 1.2-2.9%, n = 5) while peak area and height varied over a... (More)
- Fused-silica capillaries chemically derivatized with silane/poly(vinylpyrrolidone) (PVP) or dynamically modified with plexiglas [poly(methyl methacrylate)] were prepared and evaluated with regard to column stability and separation performance for capillary isoelectric focusing of standard proteins. The PVP coating showed the better stability and was good for at least 100 runs while the plexiglas coating started to deteriorate after about 30 runs. The time spent for the plexiglas coating is about 40 minutes while the PVP coating requires two days. The migration time reproducibility was better with the PVP capillary (RSD 0.7-1.6%, n = 5) compared to the plexiglas-coated column (RSD 1.2-2.9%, n = 5) while peak area and height varied over a similar interval (RSD 2-28.1% area; 0.9-22.7% height, n = 5). The two most consistent proteins in this evaluation, viz. myoglobin A and carbonic anhydrase II, showed linear dynamic ranges between 5-150 and 5-50 mug/mL, and limits of detections at 2 and 1 mug/mL, respectively, employing UV detection at 280 nm. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/288640
- author
- Palm, A ; Zaragoza-Sundqvist, M and Marko-Varga, György LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- capillary isoelectric focusing (CIEF), proteins, capillary coatings, reproducibility
- in
- Journal of Separation Science
- volume
- 27
- issue
- 1-2
- pages
- 124 - 128
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000188551400018
- pmid:15335068
- scopus:0442294234
- ISSN
- 1615-9314
- DOI
- 10.1002/jssc.200301596
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 476fc17e-ca28-41d0-97e1-5b882176b7c1 (old id 288640)
- date added to LUP
- 2016-04-01 11:42:01
- date last changed
- 2022-01-26 08:56:04
@article{476fc17e-ca28-41d0-97e1-5b882176b7c1,
abstract = {{Fused-silica capillaries chemically derivatized with silane/poly(vinylpyrrolidone) (PVP) or dynamically modified with plexiglas [poly(methyl methacrylate)] were prepared and evaluated with regard to column stability and separation performance for capillary isoelectric focusing of standard proteins. The PVP coating showed the better stability and was good for at least 100 runs while the plexiglas coating started to deteriorate after about 30 runs. The time spent for the plexiglas coating is about 40 minutes while the PVP coating requires two days. The migration time reproducibility was better with the PVP capillary (RSD 0.7-1.6%, n = 5) compared to the plexiglas-coated column (RSD 1.2-2.9%, n = 5) while peak area and height varied over a similar interval (RSD 2-28.1% area; 0.9-22.7% height, n = 5). The two most consistent proteins in this evaluation, viz. myoglobin A and carbonic anhydrase II, showed linear dynamic ranges between 5-150 and 5-50 mug/mL, and limits of detections at 2 and 1 mug/mL, respectively, employing UV detection at 280 nm.}},
author = {{Palm, A and Zaragoza-Sundqvist, M and Marko-Varga, György}},
issn = {{1615-9314}},
keywords = {{capillary isoelectric focusing (CIEF); proteins; capillary coatings; reproducibility}},
language = {{eng}},
number = {{1-2}},
pages = {{124--128}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Separation Science}},
title = {{Capillary isoelectric focusing of proteins utilizing poly(vinylpyrrolodone)- and plexigla-coated columns}},
url = {{http://dx.doi.org/10.1002/jssc.200301596}},
doi = {{10.1002/jssc.200301596}},
volume = {{27}},
year = {{2004}},
}