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In vitro sliding of actin filaments labelled with single quantum dots

Mansson, A; Sundberg, M; Balaz, M; Bunk, Richard LU ; Nicholls, I A; Omling, Pär LU ; Tagerud, S and Montelius, Lars LU (2004) In Biochemical and Biophysical Research Communications 314(2). p.529-534
Abstract
We recently refined the in vitro motility assay for studies of actomyosin function to achieve rectified myosin induced sliding of actin filaments. This paves the way, both for detailed functional studies of actomyosin and for nanotechnological applications. In the latter applications it would be desirable to use actin filaments for transportation of cargoes (e.g., enzymes) between different predetermined locations on a chip. We here describe how single quantum dot labelling of isolated actin filaments simultaneously provides handles for cargo attachment and bright and photostable fluorescence labels facilitating cargo detection and filament tracking. Labelling was achieved with preserved actomyosin function using streptavidin-coated CdSe... (More)
We recently refined the in vitro motility assay for studies of actomyosin function to achieve rectified myosin induced sliding of actin filaments. This paves the way, both for detailed functional studies of actomyosin and for nanotechnological applications. In the latter applications it would be desirable to use actin filaments for transportation of cargoes (e.g., enzymes) between different predetermined locations on a chip. We here describe how single quantum dot labelling of isolated actin filaments simultaneously provides handles for cargo attachment and bright and photostable fluorescence labels facilitating cargo detection and filament tracking. Labelling was achieved with preserved actomyosin function using streptavidin-coated CdSe quantum dots (Qdots). These nanocrystals have several unique physical properties and the present work describes their first use for functional studies of isolated proteins outside the cell. The results, in addition to the nanotechnology developments, open for new types of in vitro assays of isolated biomolecules. (C) 2003 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nanocrystal, nanostructure, quantum dot, myosin, actin, motility assay
in
Biochemical and Biophysical Research Communications
volume
314
issue
2
pages
529 - 534
publisher
Elsevier
external identifiers
  • wos:000188612800035
  • pmid:14733939
  • scopus:0347004627
ISSN
1090-2104
DOI
10.1016/j.bbrc.2003.12.133
language
English
LU publication?
yes
id
b60df51d-355a-4069-9ae4-51c7aaa1b590 (old id 288850)
date added to LUP
2007-10-25 12:51:14
date last changed
2017-10-29 04:16:22
@article{b60df51d-355a-4069-9ae4-51c7aaa1b590,
  abstract     = {We recently refined the in vitro motility assay for studies of actomyosin function to achieve rectified myosin induced sliding of actin filaments. This paves the way, both for detailed functional studies of actomyosin and for nanotechnological applications. In the latter applications it would be desirable to use actin filaments for transportation of cargoes (e.g., enzymes) between different predetermined locations on a chip. We here describe how single quantum dot labelling of isolated actin filaments simultaneously provides handles for cargo attachment and bright and photostable fluorescence labels facilitating cargo detection and filament tracking. Labelling was achieved with preserved actomyosin function using streptavidin-coated CdSe quantum dots (Qdots). These nanocrystals have several unique physical properties and the present work describes their first use for functional studies of isolated proteins outside the cell. The results, in addition to the nanotechnology developments, open for new types of in vitro assays of isolated biomolecules. (C) 2003 Elsevier Inc. All rights reserved.},
  author       = {Mansson, A and Sundberg, M and Balaz, M and Bunk, Richard and Nicholls, I A and Omling, Pär and Tagerud, S and Montelius, Lars},
  issn         = {1090-2104},
  keyword      = {nanocrystal,nanostructure,quantum dot,myosin,actin,motility assay},
  language     = {eng},
  number       = {2},
  pages        = {529--534},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {In vitro sliding of actin filaments labelled with single quantum dots},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2003.12.133},
  volume       = {314},
  year         = {2004},
}