Lectin typing of Campylobacter jejuni using a novel quartz crystal microbalance technique.
(2011) In Analytica Chimica Acta 694(1-2). p.1-5- Abstract
- Seven Campylobacter jejuni strains were characterised by a lectin typing assay. The typing system was based on a quartz crystal microbalance technique (QCM) with four commercially available lectins (wheat germ agglutinin, Maackia amurensis lectin, Lens culinaris agglutinin, and Concanavalin A), which were chosen for their differing carbohydrate specificities. Initially, the gold surfaces of the quartz crystals were modified with 11-mercaptoundecanoic acid followed by lectin immobilisation using a conventional amine-coupling technique. Bacterial cells were applied for lectin typing without preliminary treatment, and resonant frequency and dissipation responses were recorded. The adhesion of microorganisms on lectin surfaces was confirmed by... (More)
- Seven Campylobacter jejuni strains were characterised by a lectin typing assay. The typing system was based on a quartz crystal microbalance technique (QCM) with four commercially available lectins (wheat germ agglutinin, Maackia amurensis lectin, Lens culinaris agglutinin, and Concanavalin A), which were chosen for their differing carbohydrate specificities. Initially, the gold surfaces of the quartz crystals were modified with 11-mercaptoundecanoic acid followed by lectin immobilisation using a conventional amine-coupling technique. Bacterial cells were applied for lectin typing without preliminary treatment, and resonant frequency and dissipation responses were recorded. The adhesion of microorganisms on lectin surfaces was confirmed by atomic force microscopy. Scanning was performed in the tapping mode and the presence of bacteria on lectin-coated surfaces was successfully demonstrated. A significant difference in the dissipation response was observed for different C. jejuni strains which made it possible to use this parameter for discriminating between bacterial strains. In summary, the QCM technique proved a powerful tool for the recognition and discrimination of C. jejuni strains. The approach may also prove applicable to strain discrimination of other bacterial species, particularly pathogens. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1972695
- author
- Yakovleva, Maria ; Moran, Anthony P ; Safina, Gulnara ; Wadström, Torkel LU and Danielsson, Bengt
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytica Chimica Acta
- volume
- 694
- issue
- 1-2
- pages
- 1 - 5
- publisher
- Elsevier
- external identifiers
-
- wos:000291239300001
- pmid:21565296
- scopus:79955706900
- pmid:21565296
- ISSN
- 1873-4324
- DOI
- 10.1016/j.aca.2011.03.014
- language
- English
- LU publication?
- yes
- id
- 2897520b-c00c-4a12-bf43-8b164c411a7f (old id 1972695)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21565296?dopt=Abstract
- date added to LUP
- 2016-04-04 09:39:18
- date last changed
- 2022-01-29 18:54:13
@article{2897520b-c00c-4a12-bf43-8b164c411a7f,
abstract = {{Seven Campylobacter jejuni strains were characterised by a lectin typing assay. The typing system was based on a quartz crystal microbalance technique (QCM) with four commercially available lectins (wheat germ agglutinin, Maackia amurensis lectin, Lens culinaris agglutinin, and Concanavalin A), which were chosen for their differing carbohydrate specificities. Initially, the gold surfaces of the quartz crystals were modified with 11-mercaptoundecanoic acid followed by lectin immobilisation using a conventional amine-coupling technique. Bacterial cells were applied for lectin typing without preliminary treatment, and resonant frequency and dissipation responses were recorded. The adhesion of microorganisms on lectin surfaces was confirmed by atomic force microscopy. Scanning was performed in the tapping mode and the presence of bacteria on lectin-coated surfaces was successfully demonstrated. A significant difference in the dissipation response was observed for different C. jejuni strains which made it possible to use this parameter for discriminating between bacterial strains. In summary, the QCM technique proved a powerful tool for the recognition and discrimination of C. jejuni strains. The approach may also prove applicable to strain discrimination of other bacterial species, particularly pathogens.}},
author = {{Yakovleva, Maria and Moran, Anthony P and Safina, Gulnara and Wadström, Torkel and Danielsson, Bengt}},
issn = {{1873-4324}},
language = {{eng}},
number = {{1-2}},
pages = {{1--5}},
publisher = {{Elsevier}},
series = {{Analytica Chimica Acta}},
title = {{Lectin typing of Campylobacter jejuni using a novel quartz crystal microbalance technique.}},
url = {{http://dx.doi.org/10.1016/j.aca.2011.03.014}},
doi = {{10.1016/j.aca.2011.03.014}},
volume = {{694}},
year = {{2011}},
}