Secretin and its C-terminal hexapeptide potentiates insulin release in mouse islets
(1986) In American Journal of Physiology - Endocrinology and Metabolism 250(2). p.107-113- Abstract
Peptides representing the C-terminal end of secretin were synthetized and their effects tested along with secretin on column-perifused isolated mouse pancreatic islets. Insulin release induced by 10 mmol/l D-glucose was potentiated by secretin tested in a concentration range of 0.01-10 μg/ml; the maximal effect was obtained with 1 μg/ml secretin. This effect was mimicked by 50-500 μg/ml NH2-Leu-Leu-Gln-Gly-Leu-Val-NH2, [S-(22-27)], which represents an amidated C-terminal sequence of the secretin molecule. The consecutive smaller secretin C-terminal peptides had either no effects [Val-NH2, S-(24-27)] or only marginally [S-(26-27), S-(23-27)]potentiating effects on insulin release in the presence of 10... (More)
Peptides representing the C-terminal end of secretin were synthetized and their effects tested along with secretin on column-perifused isolated mouse pancreatic islets. Insulin release induced by 10 mmol/l D-glucose was potentiated by secretin tested in a concentration range of 0.01-10 μg/ml; the maximal effect was obtained with 1 μg/ml secretin. This effect was mimicked by 50-500 μg/ml NH2-Leu-Leu-Gln-Gly-Leu-Val-NH2, [S-(22-27)], which represents an amidated C-terminal sequence of the secretin molecule. The consecutive smaller secretin C-terminal peptides had either no effects [Val-NH2, S-(24-27)] or only marginally [S-(26-27), S-(23-27)]potentiating effects on insulin release in the presence of 10 mmol/l D-glucose. The effects of secretin and S-(22-27) were not influenced by 2 mmol/l glutamine. The intact hormone and the five synthetic peptides as well as Val-NH2 had no stimulatory effect on islet glutamate dehydrogenase activity. In fact, S-(23-27), S-(24-27), and S-(25-27) inhibited the islet glutamate dehydrogenase activity, the activation by which amino acids and amino acid derivatives are known to elicit a potentiation of insulin release. Our results suggest that the C-terminal part is important to the marked potentiation of glucose-induced insulin release in vitro by secretin.
(Less)
- author
- Kofod, H. ; Hansen, B. LU ; Lernmark, A. LU and Hedeskov, C. J.
- publishing date
- 1986-01-01
- type
- Contribution to journal
- publication status
- published
- in
- American Journal of Physiology - Endocrinology and Metabolism
- volume
- 250
- issue
- 2
- pages
- 107 - 113
- publisher
- American Physiological Society
- external identifiers
-
- scopus:0022552553
- pmid:3513606
- ISSN
- 0193-1849
- language
- English
- LU publication?
- no
- id
- 28c8e476-357e-43c8-a312-afd1ef129f83
- date added to LUP
- 2019-09-16 12:34:26
- date last changed
- 2024-03-13 08:03:08
@article{28c8e476-357e-43c8-a312-afd1ef129f83, abstract = {{<p>Peptides representing the C-terminal end of secretin were synthetized and their effects tested along with secretin on column-perifused isolated mouse pancreatic islets. Insulin release induced by 10 mmol/l D-glucose was potentiated by secretin tested in a concentration range of 0.01-10 μg/ml; the maximal effect was obtained with 1 μg/ml secretin. This effect was mimicked by 50-500 μg/ml NH<sub>2</sub>-Leu-Leu-Gln-Gly-Leu-Val-NH<sub>2</sub>, [S-(22-27)], which represents an amidated C-terminal sequence of the secretin molecule. The consecutive smaller secretin C-terminal peptides had either no effects [Val-NH<sub>2</sub>, S-(24-27)] or only marginally [S-(26-27), S-(23-27)]potentiating effects on insulin release in the presence of 10 mmol/l D-glucose. The effects of secretin and S-(22-27) were not influenced by 2 mmol/l glutamine. The intact hormone and the five synthetic peptides as well as Val-NH<sub>2</sub> had no stimulatory effect on islet glutamate dehydrogenase activity. In fact, S-(23-27), S-(24-27), and S-(25-27) inhibited the islet glutamate dehydrogenase activity, the activation by which amino acids and amino acid derivatives are known to elicit a potentiation of insulin release. Our results suggest that the C-terminal part is important to the marked potentiation of glucose-induced insulin release in vitro by secretin.</p>}}, author = {{Kofod, H. and Hansen, B. and Lernmark, A. and Hedeskov, C. J.}}, issn = {{0193-1849}}, language = {{eng}}, month = {{01}}, number = {{2}}, pages = {{107--113}}, publisher = {{American Physiological Society}}, series = {{American Journal of Physiology - Endocrinology and Metabolism}}, title = {{Secretin and its C-terminal hexapeptide potentiates insulin release in mouse islets}}, volume = {{250}}, year = {{1986}}, }