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Effects of pentobarbital on GABA-activated currents in acutely-isolated rat dentate gyrus granule neurons

Lim, MSF; Lindquist, Catarina LU and Birnir, Bryndis LU (2003) In Neuroscience Letters 353(2). p.139-142
Abstract
Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 muM gamma-aminobutyric acid (GABA; EC30) evoked currents was obtained with 100 muM pentobarbital where the peak-current was 2.1 +/- 0.2 of control. One hundred muM pentobarbital alone evoked no current response whereas I mM pentobarbital elicited a current response that was 0.4 +/- 0.2 of the 100 muM GABA-activated peak current. In 100 muM pentobarbital, the GABA EC50 value shifted from 14 to 3 muM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 muM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 muM... (More)
Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 muM gamma-aminobutyric acid (GABA; EC30) evoked currents was obtained with 100 muM pentobarbital where the peak-current was 2.1 +/- 0.2 of control. One hundred muM pentobarbital alone evoked no current response whereas I mM pentobarbital elicited a current response that was 0.4 +/- 0.2 of the 100 muM GABA-activated peak current. In 100 muM pentobarbital, the GABA EC50 value shifted from 14 to 3 muM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 muM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 muM GABA. The results show that in the granule neurons the drug potency differs for the different effects of pentobarbital at GABA(A) receptors with the modulatory and inhibitory effects requiring lower concentrations than the direct activation of the receptors. (C) 2003 Elsevier Ireland Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
receptor, inhibition, barbiturates, gamma-aminobutyric acid(A), hippocampus, ion channel
in
Neuroscience Letters
volume
353
issue
2
pages
139 - 142
publisher
Elsevier
external identifiers
  • pmid:14664920
  • wos:000186903000016
  • scopus:0345096615
ISSN
0304-3940
DOI
10.1016/j.neulet.2003.09.025
language
English
LU publication?
yes
id
c4385213-646d-4336-9995-3ef81830439d (old id 293782)
date added to LUP
2007-09-17 09:02:48
date last changed
2018-10-04 13:36:14
@article{c4385213-646d-4336-9995-3ef81830439d,
  abstract     = {Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 muM gamma-aminobutyric acid (GABA; EC30) evoked currents was obtained with 100 muM pentobarbital where the peak-current was 2.1 +/- 0.2 of control. One hundred muM pentobarbital alone evoked no current response whereas I mM pentobarbital elicited a current response that was 0.4 +/- 0.2 of the 100 muM GABA-activated peak current. In 100 muM pentobarbital, the GABA EC50 value shifted from 14 to 3 muM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 muM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 muM GABA. The results show that in the granule neurons the drug potency differs for the different effects of pentobarbital at GABA(A) receptors with the modulatory and inhibitory effects requiring lower concentrations than the direct activation of the receptors. (C) 2003 Elsevier Ireland Ltd. All rights reserved.},
  author       = {Lim, MSF and Lindquist, Catarina and Birnir, Bryndis},
  issn         = {0304-3940},
  keyword      = {receptor,inhibition,barbiturates,gamma-aminobutyric acid(A),hippocampus,ion channel},
  language     = {eng},
  number       = {2},
  pages        = {139--142},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Effects of pentobarbital on GABA-activated currents in acutely-isolated rat dentate gyrus granule neurons},
  url          = {http://dx.doi.org/10.1016/j.neulet.2003.09.025},
  volume       = {353},
  year         = {2003},
}