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Silanized surfaces for in vitro studies of actomyosin function and nanotechnology applications

Sundberg, M; Rosengren, JP; Bunk, Richard LU ; Lindahl, J; Nicholls, IA; Tagerud, S; Omling, Pär LU ; Montelius, Lars LU and Mansson, A (2003) In Analytical Biochemistry 323(1). p.127-138
Abstract
We have previously shown that selective heavy meromyosin (HMM) adsorption to predefined regions of nanostructured polymer resist surfaces may be used to produce a nanostructured in vitro motility assay. However, actomyosin function was of lower quality than on conventional nitrocellulose films. We have therefore studied actomyosin function on differently derivatized glass surfaces with the aim to find a substitute for the polymer resists. We have found that surfaces derivatized with trimethylchlorosilane (TMCS) were superior to all other surfaces tested, including nitrocellulose. High-quality actin filament motility was observed up to 6 days after incubation with HMM and the fraction of motile actin filaments and the velocity of smooth... (More)
We have previously shown that selective heavy meromyosin (HMM) adsorption to predefined regions of nanostructured polymer resist surfaces may be used to produce a nanostructured in vitro motility assay. However, actomyosin function was of lower quality than on conventional nitrocellulose films. We have therefore studied actomyosin function on differently derivatized glass surfaces with the aim to find a substitute for the polymer resists. We have found that surfaces derivatized with trimethylchlorosilane (TMCS) were superior to all other surfaces tested, including nitrocellulose. High-quality actin filament motility was observed up to 6 days after incubation with HMM and the fraction of motile actin filaments and the velocity of smooth sliding were generally higher on TMCS than on nitrocellulose. The actomyosin function on TMCS-derivatized glass and nitrocellulose is considered in relation to roughness and hydrophobicity of these surfaces. The results suggest that TMCS is an ideal substitute for polymer resists in the nanostructured in vitro motility assay. Furthermore, TMCS derivatized glass also seems to offer several advantages over nitrocellulose for HMM adsorption in the ordinary in vitro motility assay. (C) 2003 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
motor protein, molecular motor, actomyosin, nitrocellulose, dimethyldichlorosilane, octadecyltrichlorosilane, heavy meromyosin, trimethylehlorosilane
in
Analytical Biochemistry
volume
323
issue
1
pages
127 - 138
publisher
Elsevier
external identifiers
  • wos:000186822700017
  • pmid:14622967
  • scopus:0242521629
ISSN
1096-0309
DOI
10.1016/j.ab.2003.07.022
language
English
LU publication?
yes
id
92f76855-1f65-476d-89c4-2a535d1b4c9c (old id 294707)
date added to LUP
2007-09-22 13:42:52
date last changed
2018-01-07 05:48:43
@article{92f76855-1f65-476d-89c4-2a535d1b4c9c,
  abstract     = {We have previously shown that selective heavy meromyosin (HMM) adsorption to predefined regions of nanostructured polymer resist surfaces may be used to produce a nanostructured in vitro motility assay. However, actomyosin function was of lower quality than on conventional nitrocellulose films. We have therefore studied actomyosin function on differently derivatized glass surfaces with the aim to find a substitute for the polymer resists. We have found that surfaces derivatized with trimethylchlorosilane (TMCS) were superior to all other surfaces tested, including nitrocellulose. High-quality actin filament motility was observed up to 6 days after incubation with HMM and the fraction of motile actin filaments and the velocity of smooth sliding were generally higher on TMCS than on nitrocellulose. The actomyosin function on TMCS-derivatized glass and nitrocellulose is considered in relation to roughness and hydrophobicity of these surfaces. The results suggest that TMCS is an ideal substitute for polymer resists in the nanostructured in vitro motility assay. Furthermore, TMCS derivatized glass also seems to offer several advantages over nitrocellulose for HMM adsorption in the ordinary in vitro motility assay. (C) 2003 Elsevier Inc. All rights reserved.},
  author       = {Sundberg, M and Rosengren, JP and Bunk, Richard and Lindahl, J and Nicholls, IA and Tagerud, S and Omling, Pär and Montelius, Lars and Mansson, A},
  issn         = {1096-0309},
  keyword      = {motor protein,molecular motor,actomyosin,nitrocellulose,dimethyldichlorosilane,octadecyltrichlorosilane,heavy meromyosin,trimethylehlorosilane},
  language     = {eng},
  number       = {1},
  pages        = {127--138},
  publisher    = {Elsevier},
  series       = {Analytical Biochemistry},
  title        = {Silanized surfaces for in vitro studies of actomyosin function and nanotechnology applications},
  url          = {http://dx.doi.org/10.1016/j.ab.2003.07.022},
  volume       = {323},
  year         = {2003},
}