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Tumor specific VEGF-A and VEGFR2/KDR protein are co-expressed in breast cancer

Ryden, L; Linderholm, B; Nielsen, NH; Emdin, S; Jonsson, PE and Landberg, Göran LU (2003) In Breast Cancer Research and Treatment 82(3). p.147-154
Abstract
Angiogenesis is a prognostic indicator in primary breast cancer regulated by specific angiogenic factors and their receptors. Vascular endothelial growth factor-A (VEGF-A), so far considered the most important, acts through dimerization of the receptor VEGFR2/KDR within the receptor tyrosine kinase family of VEGF receptors. In order to study the interplay between VEGF-A and VEGFR2/KDR in breast cancer we evaluated their expression by immunohistochemistry in 102 breast cancers organized in a tumor tissue array system allowing semi-quantitative evaluation of cytoplasmatic staining intensity. In addition, VEGF-A(165) was analyzed by an enzyme immuno assay (ELISA) in protein extracts prepared from frozen tissue from 98 of 102 tumors included... (More)
Angiogenesis is a prognostic indicator in primary breast cancer regulated by specific angiogenic factors and their receptors. Vascular endothelial growth factor-A (VEGF-A), so far considered the most important, acts through dimerization of the receptor VEGFR2/KDR within the receptor tyrosine kinase family of VEGF receptors. In order to study the interplay between VEGF-A and VEGFR2/KDR in breast cancer we evaluated their expression by immunohistochemistry in 102 breast cancers organized in a tumor tissue array system allowing semi-quantitative evaluation of cytoplasmatic staining intensity. In addition, VEGF-A(165) was analyzed by an enzyme immuno assay (ELISA) in protein extracts prepared from frozen tissue from 98 of 102 tumors included in the array. Cytoplasmatic staining of VEGF of varying intensity was observed in all samples and correlated with the ELISA results of VEGF content (p=0.007). Interestingly, VEGFR2/KDR expression correlated with VEGF expression using immunohistochemistry, indicating that VEGF and VEGFR2/KDR may be co-expressed in breast cancer. Furthermore, high levels of VEGF-A(165) in the protein extracts was associated with impaired short time survival but not long term survival whereas immunohistochemically assessed VEGF and VEGFR2/KDR were not significantly associated with survival. In summary, immunohistochemically based analysis of VEGF using a tumor tissue array system seems to be a useful method for VEGF quantification in breast cancer here validated using an ELISA based method. The tumor tissue array system enables opportunities of simultaneous analysis of markers engaged in angiogenesis justifying further studies using larger series of tumors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
VEGFR2/KDR, VEGF-A, prognosis, angiogenesis, breast cancer
in
Breast Cancer Research and Treatment
volume
82
issue
3
pages
147 - 154
publisher
Springer
external identifiers
  • wos:000186636500001
  • scopus:0346244031
ISSN
1573-7217
DOI
language
English
LU publication?
yes
id
59603410-e419-45bc-bb5d-1e515196cec3 (old id 295478)
date added to LUP
2007-09-21 09:59:26
date last changed
2018-06-10 04:46:58
@article{59603410-e419-45bc-bb5d-1e515196cec3,
  abstract     = {Angiogenesis is a prognostic indicator in primary breast cancer regulated by specific angiogenic factors and their receptors. Vascular endothelial growth factor-A (VEGF-A), so far considered the most important, acts through dimerization of the receptor VEGFR2/KDR within the receptor tyrosine kinase family of VEGF receptors. In order to study the interplay between VEGF-A and VEGFR2/KDR in breast cancer we evaluated their expression by immunohistochemistry in 102 breast cancers organized in a tumor tissue array system allowing semi-quantitative evaluation of cytoplasmatic staining intensity. In addition, VEGF-A(165) was analyzed by an enzyme immuno assay (ELISA) in protein extracts prepared from frozen tissue from 98 of 102 tumors included in the array. Cytoplasmatic staining of VEGF of varying intensity was observed in all samples and correlated with the ELISA results of VEGF content (p=0.007). Interestingly, VEGFR2/KDR expression correlated with VEGF expression using immunohistochemistry, indicating that VEGF and VEGFR2/KDR may be co-expressed in breast cancer. Furthermore, high levels of VEGF-A(165) in the protein extracts was associated with impaired short time survival but not long term survival whereas immunohistochemically assessed VEGF and VEGFR2/KDR were not significantly associated with survival. In summary, immunohistochemically based analysis of VEGF using a tumor tissue array system seems to be a useful method for VEGF quantification in breast cancer here validated using an ELISA based method. The tumor tissue array system enables opportunities of simultaneous analysis of markers engaged in angiogenesis justifying further studies using larger series of tumors.},
  author       = {Ryden, L and Linderholm, B and Nielsen, NH and Emdin, S and Jonsson, PE and Landberg, Göran},
  issn         = {1573-7217},
  keyword      = {VEGFR2/KDR,VEGF-A,prognosis,angiogenesis,breast cancer},
  language     = {eng},
  number       = {3},
  pages        = {147--154},
  publisher    = {Springer},
  series       = {Breast Cancer Research and Treatment},
  title        = {Tumor specific VEGF-A and VEGFR2/KDR protein are co-expressed in breast cancer},
  url          = {http://dx.doi.org/},
  volume       = {82},
  year         = {2003},
}