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Pedobacter lusitanus sp. nov., isolated from sludge of a deactivated uranium mine

Covas, Cláudia ; Caetano, Tânia ; Cruz, Andreia ; Santos, Tiago ; Dias, Liliana ; Klein, Guenter ; Abdulmawjood, Amir ; Rodríguez-Alcalá, Luis M. ; Pimentel, Lígia L. and Gomes, Ana , et al. (2017) In International Journal of Systematic and Evolutionary Microbiology 67(5). p.1339-1348
Abstract

Strain NL19T is a Gram-stain-negative, aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T. It had a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids included C16: 1ω7c, C14: 1ω5c, C4: 0, iso-C17: 0, iso-C17: 0 3-OH, C16: 0, anteiso-C15: 0 and... (More)

Strain NL19T is a Gram-stain-negative, aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T. It had a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids included C16: 1ω7c, C14: 1ω5c, C4: 0, iso-C17: 0, iso-C17: 0 3-OH, C16: 0, anteiso-C15: 0 and iso- C15: 0 3-OH. The main lipids present were phospholipids (60%) and sphingolipids (35%). The most abundant phospholipids included phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine. Menaquinone-7 (MK-7) was the only isoprenoid quinone detected. DNA–DNA hybridization similarities between strain NL19T and Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T were 15.3, 16.2, 11.5 and 16.0%, respectively. Strain NL19T can also be distinguished from these four species based on gyrB and intergenic transcribed spacers (ITS) sequences and by some phenotypic traits such as NaCl tolerance, pH, growth temperature and carbon source utilization. Strain NL19T represents a novel species of the genus Pedobacter, for which the name Pedobacter lusitanus sp. nov. is proposed. The type strain is NL19T (=LMG 29220T=CECT 9028T). An amended description of Pedobacter himalayensis is also included.

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type
Contribution to journal
publication status
published
subject
keywords
16S rRNA gene, Antibacterial activity, GyrB, Intergenic transcribed spacer, Phospholipids and sphingolipids, Polyphasic taxonomy
in
International Journal of Systematic and Evolutionary Microbiology
volume
67
issue
5
article number
001814
pages
10 pages
publisher
Society for General Microbiology
external identifiers
  • pmid:28109203
  • scopus:85020441779
ISSN
1466-5026
DOI
10.1099/ijsem.0.001814
language
English
LU publication?
no
id
296485a6-5bb6-45b7-8551-51e8ee840911
date added to LUP
2020-02-05 15:16:25
date last changed
2021-02-17 04:48:29
@article{296485a6-5bb6-45b7-8551-51e8ee840911,
  abstract     = {<p>Strain NL19<sup>T</sup> is a Gram-stain-negative, aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19<sup>T</sup> is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384<sup>T</sup>, Pedobacter cryoconitis DSM 14825<sup>T</sup>, Pedobacter westerhofensis DSM 19036<sup>T</sup> and Pedobacter hartonius DSM 19033<sup>T</sup>. It had a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids included C<sub>16: 1</sub>ω7c, C<sub>14: 1</sub>ω5c, C<sub>4: 0</sub>, iso-C<sub>17: 0</sub>, iso-C<sub>17: 0</sub> 3-OH, C<sub>16: 0</sub>, anteiso-C<sub>15: 0</sub> and iso- C<sub>15: 0</sub> 3-OH. The main lipids present were phospholipids (60%) and sphingolipids (35%). The most abundant phospholipids included phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine. Menaquinone-7 (MK-7) was the only isoprenoid quinone detected. DNA–DNA hybridization similarities between strain NL19<sup>T</sup> and Pedobacter himalayensis MTCC 6384<sup>T</sup>, Pedobacter cryoconitis DSM 14825<sup>T</sup>, Pedobacter westerhofensis DSM 19036<sup>T</sup> and Pedobacter hartonius DSM 19033<sup>T</sup> were 15.3, 16.2, 11.5 and 16.0%, respectively. Strain NL19<sup>T</sup> can also be distinguished from these four species based on gyrB and intergenic transcribed spacers (ITS) sequences and by some phenotypic traits such as NaCl tolerance, pH, growth temperature and carbon source utilization. Strain NL19<sup>T</sup> represents a novel species of the genus Pedobacter, for which the name Pedobacter lusitanus sp. nov. is proposed. The type strain is NL19<sup>T</sup> (=LMG 29220<sup>T</sup>=CECT 9028<sup>T</sup>). An amended description of Pedobacter himalayensis is also included.</p>},
  author       = {Covas, Cláudia and Caetano, Tânia and Cruz, Andreia and Santos, Tiago and Dias, Liliana and Klein, Guenter and Abdulmawjood, Amir and Rodríguez-Alcalá, Luis M. and Pimentel, Lígia L. and Gomes, Ana and Freitas, Ana Cristina and Garcia-Serrano, Alba and Fontecha, Javier and Mendo, Sónia},
  issn         = {1466-5026},
  language     = {eng},
  month        = {05},
  number       = {5},
  pages        = {1339--1348},
  publisher    = {Society for General Microbiology},
  series       = {International Journal of Systematic and Evolutionary Microbiology},
  title        = {Pedobacter lusitanus sp. nov., isolated from sludge of a deactivated uranium mine},
  url          = {http://dx.doi.org/10.1099/ijsem.0.001814},
  doi          = {10.1099/ijsem.0.001814},
  volume       = {67},
  year         = {2017},
}