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Improved DNA flow cytometric, DNA ploidy, and S-phase reproducibility between 15 laboratories in analysis of breast cancer using generalized guidelines

Baldetorp, Bo LU ; Bendahl, Pär-Ola LU ; Fernö, Mårten LU and Stal, O (2003) In Cytometry 56A(1). p.1-7
Abstract
Background: Lack of generalized guidelines for DNA flow cytometric analysis (FCM) may be the main reason for its limited use in the clinical management of breast cancer. Methods: After an initial interlaboratory reproducibility study (Round 1), we concluded that it was the evaluation of the DNA histograms rather than the technical performance of the analysis that was the main reason for discordant results between laboratories. Guidelines for the interpretation of DNA histograms were therefore drawn up. We present here data from a new reproducibility study (Round 11) using these guidelines. Results: For 10 laboratories also participating in Round 1, use of the guidelines increased the concordance in DNA ploidy status from 89% to 100% for... (More)
Background: Lack of generalized guidelines for DNA flow cytometric analysis (FCM) may be the main reason for its limited use in the clinical management of breast cancer. Methods: After an initial interlaboratory reproducibility study (Round 1), we concluded that it was the evaluation of the DNA histograms rather than the technical performance of the analysis that was the main reason for discordant results between laboratories. Guidelines for the interpretation of DNA histograms were therefore drawn up. We present here data from a new reproducibility study (Round 11) using these guidelines. Results: For 10 laboratories also participating in Round 1, use of the guidelines increased the concordance in DNA ploidy status from 89% to 100% for the 46 samples used in both rounds. The concordance rate for SPF also increased; mean r(s)-value increased from 0.81 to 0.88, and mean kappa value (lower two-thirds versus upper third versus not reported) increased from 0.55 to 0.71. Five new laboratories, participating only in Round II, also agreed with the 10 original laboratories regarding DNA ploidy status. With the inclusion of all 15 laboratories, we obtained a mean r(s)-value of 0.81 and a mean kappa value of 0.72 for SPF. Conclusions: Generalized guidelines for DNA FCM increase interlaboratory agreement, which is highly important in clinical routines and in multicenter studies. Furthermore, inexperienced FCM laboratories using generalized guidelines can produce and interpret DNA FCM data equally as well as experienced laboratories. Cytometry Part A 56A:1-7, 2003. (C) 2003 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DNA-ploidy, S-phase fraction, breast cancer, DNA flow cytometry, reproducibility
in
Cytometry
volume
56A
issue
1
pages
1 - 7
publisher
John Wiley & Sons
external identifiers
  • pmid:14566933
  • wos:000186285400001
  • scopus:0347504891
ISSN
0196-4763
DOI
10.1002/cyto.a.10083
language
English
LU publication?
yes
id
333edb8a-5eb4-471b-9134-6739d008c325 (old id 296526)
date added to LUP
2007-08-22 14:46:15
date last changed
2017-01-01 06:43:40
@article{333edb8a-5eb4-471b-9134-6739d008c325,
  abstract     = {Background: Lack of generalized guidelines for DNA flow cytometric analysis (FCM) may be the main reason for its limited use in the clinical management of breast cancer. Methods: After an initial interlaboratory reproducibility study (Round 1), we concluded that it was the evaluation of the DNA histograms rather than the technical performance of the analysis that was the main reason for discordant results between laboratories. Guidelines for the interpretation of DNA histograms were therefore drawn up. We present here data from a new reproducibility study (Round 11) using these guidelines. Results: For 10 laboratories also participating in Round 1, use of the guidelines increased the concordance in DNA ploidy status from 89% to 100% for the 46 samples used in both rounds. The concordance rate for SPF also increased; mean r(s)-value increased from 0.81 to 0.88, and mean kappa value (lower two-thirds versus upper third versus not reported) increased from 0.55 to 0.71. Five new laboratories, participating only in Round II, also agreed with the 10 original laboratories regarding DNA ploidy status. With the inclusion of all 15 laboratories, we obtained a mean r(s)-value of 0.81 and a mean kappa value of 0.72 for SPF. Conclusions: Generalized guidelines for DNA FCM increase interlaboratory agreement, which is highly important in clinical routines and in multicenter studies. Furthermore, inexperienced FCM laboratories using generalized guidelines can produce and interpret DNA FCM data equally as well as experienced laboratories. Cytometry Part A 56A:1-7, 2003. (C) 2003 Wiley-Liss, Inc.},
  author       = {Baldetorp, Bo and Bendahl, Pär-Ola and Fernö, Mårten and Stal, O},
  issn         = {0196-4763},
  keyword      = {DNA-ploidy,S-phase fraction,breast cancer,DNA flow cytometry,reproducibility},
  language     = {eng},
  number       = {1},
  pages        = {1--7},
  publisher    = {John Wiley & Sons},
  series       = {Cytometry},
  title        = {Improved DNA flow cytometric, DNA ploidy, and S-phase reproducibility between 15 laboratories in analysis of breast cancer using generalized guidelines},
  url          = {http://dx.doi.org/10.1002/cyto.a.10083},
  volume       = {56A},
  year         = {2003},
}