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Intestinal Mucosa Reconstitution and Protection: Impact of Luminal Factors

Mao, Yilei LU (1997) In Bulletin from the Department of Surgery, University of Lund 111.
Abstract
The gastrointestinal mucosa is one of the most rapidly proliferating tissues in the body, and serves as a barrier to luminal bacteria and toxins. Enteral nutrition, luminal fiber and probiotics are of crucial importance for the integrity of this barrier. We examined the potential beneficial effects of lactobacilli and fiber on methotrexate-induced enterocolitis and radiation-induced intestinal injury in this study. Results indicate that pectin and oatbase significantly enhance the mucosal nutritional status and intestinal barrier function in the enterocolitis rats. The effects of fiber are greater in colon than in small bowel. Administration of lactobacilli to the enterocolitis rats decreases the inflammatory response, improves the mucosal... (More)
The gastrointestinal mucosa is one of the most rapidly proliferating tissues in the body, and serves as a barrier to luminal bacteria and toxins. Enteral nutrition, luminal fiber and probiotics are of crucial importance for the integrity of this barrier. We examined the potential beneficial effects of lactobacilli and fiber on methotrexate-induced enterocolitis and radiation-induced intestinal injury in this study. Results indicate that pectin and oatbase significantly enhance the mucosal nutritional status and intestinal barrier function in the enterocolitis rats. The effects of fiber are greater in colon than in small bowel. Administration of lactobacilli to the enterocolitis rats decreases the inflammatory response, improves the mucosal architecture, mucosal mass, and intestinal barrier function by restoring the intestinal permeability and microecology, reducing bacterial translocation and endotoxemia. Among five tested Lactobacillus strains, Lactobacillus plantarum (both DSM 9843 and DSM 6595) show the best effects. Lactobacilli with fermentation have greater effects than those without fermentation, and lactobacilli both with and without fermentation have bigger impact than oat fiber alone. Supplementation of Lactobacillus plantarum DSM 9843 and Lactobacillus reuteri R2LC, but not fiber, improves the intestinal mucosal immunity by increasing sIgA level and T-helper/T-suppressor lymphocyte populations. Administration of lactobacilli and oatbase to the rats following radiation and colon resection significantly improves the host general conditions, decreases the intestinal inflammatory response, and accelerate the colonic anastomotic healing. Enteric administration of lactobacilli and fiber, as a "biological approach", is of value to rats under different stress conditions. They can be used in future bacteriotherapy for various human diseases. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Docent Påhlman, Lars, Department of Surgery, Academic Hospital, Uppsala
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Probiotics, Pectin, Oat, Methotrexate, Lactobacilli, Intestinal injury, Intestinal immunity, Intestinal barrier, Enterocolitis, Fiber, Radiation, Surgery, orthopaedics, traumatology, Kirurgi, ortopedi, traumatologi
in
Bulletin from the Department of Surgery, University of Lund
volume
111
pages
189 pages
publisher
Department of Surgery, Clinical Sciences Lund, Lund University
defense location
Lecture room, Department of Surgery, University Hospital MAS, Malmö
defense date
1997-05-27 13:00
external identifiers
  • other:ISRN: LUMEDW/MESL--1099--SE
ISSN
0346-5934
language
English
LU publication?
yes
id
661eaf2d-feb7-4fe3-afac-ce664b3d0d6c (old id 29688)
date added to LUP
2007-06-13 14:42:10
date last changed
2016-09-19 08:44:57
@phdthesis{661eaf2d-feb7-4fe3-afac-ce664b3d0d6c,
  abstract     = {The gastrointestinal mucosa is one of the most rapidly proliferating tissues in the body, and serves as a barrier to luminal bacteria and toxins. Enteral nutrition, luminal fiber and probiotics are of crucial importance for the integrity of this barrier. We examined the potential beneficial effects of lactobacilli and fiber on methotrexate-induced enterocolitis and radiation-induced intestinal injury in this study. Results indicate that pectin and oatbase significantly enhance the mucosal nutritional status and intestinal barrier function in the enterocolitis rats. The effects of fiber are greater in colon than in small bowel. Administration of lactobacilli to the enterocolitis rats decreases the inflammatory response, improves the mucosal architecture, mucosal mass, and intestinal barrier function by restoring the intestinal permeability and microecology, reducing bacterial translocation and endotoxemia. Among five tested Lactobacillus strains, Lactobacillus plantarum (both DSM 9843 and DSM 6595) show the best effects. Lactobacilli with fermentation have greater effects than those without fermentation, and lactobacilli both with and without fermentation have bigger impact than oat fiber alone. Supplementation of Lactobacillus plantarum DSM 9843 and Lactobacillus reuteri R2LC, but not fiber, improves the intestinal mucosal immunity by increasing sIgA level and T-helper/T-suppressor lymphocyte populations. Administration of lactobacilli and oatbase to the rats following radiation and colon resection significantly improves the host general conditions, decreases the intestinal inflammatory response, and accelerate the colonic anastomotic healing. Enteric administration of lactobacilli and fiber, as a "biological approach", is of value to rats under different stress conditions. They can be used in future bacteriotherapy for various human diseases.},
  author       = {Mao, Yilei},
  issn         = {0346-5934},
  keyword      = {Probiotics,Pectin,Oat,Methotrexate,Lactobacilli,Intestinal injury,Intestinal immunity,Intestinal barrier,Enterocolitis,Fiber,Radiation,Surgery,orthopaedics,traumatology,Kirurgi,ortopedi,traumatologi},
  language     = {eng},
  pages        = {189},
  publisher    = {Department of Surgery, Clinical Sciences Lund, Lund University},
  school       = {Lund University},
  series       = {Bulletin from the Department of Surgery, University of Lund},
  title        = {Intestinal Mucosa Reconstitution and Protection: Impact of Luminal Factors},
  volume       = {111},
  year         = {1997},
}