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Effect of temperature and pressure on the proteolytic specificity of the recombinant 20S proteasome from Methanococcus jannaschii

Frankenberg, R J; Andersson, Maria LU and Clark, D S (2003) In Extremophiles 7(5). p.353-360
Abstract
The hydrolytic specificity of the recombinant 20S proteasom from the deep-sea thermophile Methanococcus jannaschii was evaluated toward oxidized insulin B-chain across a range of temperatures (35degrees, 55degrees. 75degrees, and 90degreesC) and hydrostatic pressures (1, 250, 500, and 1,000 atm). Of the four temperatures considered, the same maximum overall hydrolysis rate was observed at both 55degrees and 75degreesC, which are much lower than the T-opt of 116degreesC previously observed for a small amide substrate (Michels and Clark 1997). At 35degreesC the rates of cleavage were highest at the carboxyl side of glutamine and leucine, whereas at the three higher temperatures, the most rapid cleavages occurred after leucine and glutamic... (More)
The hydrolytic specificity of the recombinant 20S proteasom from the deep-sea thermophile Methanococcus jannaschii was evaluated toward oxidized insulin B-chain across a range of temperatures (35degrees, 55degrees. 75degrees, and 90degreesC) and hydrostatic pressures (1, 250, 500, and 1,000 atm). Of the four temperatures considered, the same maximum overall hydrolysis rate was observed at both 55degrees and 75degreesC, which are much lower than the T-opt of 116degreesC previously observed for a small amide substrate (Michels and Clark 1997). At 35degreesC the rates of cleavage were highest at the carboxyl side of glutamine and leucine, whereas at the three higher temperatures, the most rapid cleavages occurred after leucine and glutamic acid residues. The distribution of proteolytic fragments and the cleavage sequence also varied between the lowest and higher temperatures. Application of hydrostatic pressure did not increase proteasome activity, as observed previously for the amide substrate (Michels and Clark 1997). but instead significantly reduced the overall conversion of the polypeptide substrate. Overall cleavage patterns observed for the recombinant M. jannaschii proteasome were similar to those reported previously for Thermoplasma acidophilum (Akopian et al. 1997) and human proteasomes (Dick et al. 1991). indicating that proteasome specificity has been conserved despite significant environmental diversity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
thermophilic proteasome, Methanococcus jannaschii, high pressure, hydrolytic specificity
in
Extremophiles
volume
7
issue
5
pages
353 - 360
publisher
Springer
external identifiers
  • pmid:12820035
  • wos:000186179400003
  • scopus:3042742757
ISSN
1433-4909
DOI
language
English
LU publication?
yes
id
501710c8-c56e-4d06-b9c2-77a49b906fc5 (old id 297283)
date added to LUP
2007-09-03 08:45:41
date last changed
2018-05-29 09:38:21
@article{501710c8-c56e-4d06-b9c2-77a49b906fc5,
  abstract     = {The hydrolytic specificity of the recombinant 20S proteasom from the deep-sea thermophile Methanococcus jannaschii was evaluated toward oxidized insulin B-chain across a range of temperatures (35degrees, 55degrees. 75degrees, and 90degreesC) and hydrostatic pressures (1, 250, 500, and 1,000 atm). Of the four temperatures considered, the same maximum overall hydrolysis rate was observed at both 55degrees and 75degreesC, which are much lower than the T-opt of 116degreesC previously observed for a small amide substrate (Michels and Clark 1997). At 35degreesC the rates of cleavage were highest at the carboxyl side of glutamine and leucine, whereas at the three higher temperatures, the most rapid cleavages occurred after leucine and glutamic acid residues. The distribution of proteolytic fragments and the cleavage sequence also varied between the lowest and higher temperatures. Application of hydrostatic pressure did not increase proteasome activity, as observed previously for the amide substrate (Michels and Clark 1997). but instead significantly reduced the overall conversion of the polypeptide substrate. Overall cleavage patterns observed for the recombinant M. jannaschii proteasome were similar to those reported previously for Thermoplasma acidophilum (Akopian et al. 1997) and human proteasomes (Dick et al. 1991). indicating that proteasome specificity has been conserved despite significant environmental diversity.},
  author       = {Frankenberg, R J and Andersson, Maria and Clark, D S},
  issn         = {1433-4909},
  keyword      = {thermophilic proteasome,Methanococcus jannaschii,high pressure,hydrolytic specificity},
  language     = {eng},
  number       = {5},
  pages        = {353--360},
  publisher    = {Springer},
  series       = {Extremophiles},
  title        = {Effect of temperature and pressure on the proteolytic specificity of the recombinant 20S proteasome from Methanococcus jannaschii},
  url          = {http://dx.doi.org/},
  volume       = {7},
  year         = {2003},
}