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Role for laminin-alpha 5 chain LG4 module in epithelial branching morphogenesis

Kadoya, Y; Mochizuki, M; Nomizu, M; Sorokin, Lydia LU and Yamashina, S (2003) In Developmental Biology 263(1). p.153-164
Abstract
Laminin-alpha5 chain was localized in all epithelial basement membranes (BMs) of mouse submandibular gland (SMG) from the onset of branching morphogenesis and became restricted to BMs of epithelial ducts in the adult. To investigate whether the laminin-alpha5 chain plays a role in branching morphogenesis, a set of cell-adhesive peptides from the C-terminal globular domains (LG1-5) was tested for their effects in SMG organ cultures. One peptide, LVLFLNHGH (A5G77f), which represents a sequence located in the connecting loop between strands E and F of LG4. perturbed branching morphogenesis and resulted in irregularities in the contours of epithelial structures, with formation of deep clefts. The data suggest a role for the laminin-alpha5 LG4... (More)
Laminin-alpha5 chain was localized in all epithelial basement membranes (BMs) of mouse submandibular gland (SMG) from the onset of branching morphogenesis and became restricted to BMs of epithelial ducts in the adult. To investigate whether the laminin-alpha5 chain plays a role in branching morphogenesis, a set of cell-adhesive peptides from the C-terminal globular domains (LG1-5) was tested for their effects in SMG organ cultures. One peptide, LVLFLNHGH (A5G77f), which represents a sequence located in the connecting loop between strands E and F of LG4. perturbed branching morphogenesis and resulted in irregularities in the contours of epithelial structures, with formation of deep clefts. The data suggest a role for the laminin-alpha5 LG4 module in the development of the duct system, rather than in the bifurcation of epithelial clusters. The epithelial BM of A5G77f-peptide-treated explants was continuous, which was in contrast to our previous finding of impaired epithelial BM assembly in explants treated with the larninin-alpha1 LG4 module peptide, or with a monoclonal antibody against this domain. A5G77f also perturbed in vitro development of lung and kidney. These results suggest a crucial role for the LG4 module of larninin-alpha5 in epithelial morphogenesis that is distinct from that of the laminin-alpha1 LG4. (C) 2003 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
peptide, submandibular gland, organ culture, laminin-alpha 5, laminin-alpha chain, immunohistochemistry, basement membrane, branching morphogenesis
in
Developmental Biology
volume
263
issue
1
pages
153 - 164
publisher
Elsevier
external identifiers
  • wos:000186245100012
  • pmid:14568553
  • scopus:0142042869
ISSN
1095-564X
DOI
language
English
LU publication?
yes
id
c2309550-ab9f-4be9-82d6-5134569c8116 (old id 297287)
date added to LUP
2007-09-13 15:07:43
date last changed
2018-05-29 10:28:44
@article{c2309550-ab9f-4be9-82d6-5134569c8116,
  abstract     = {Laminin-alpha5 chain was localized in all epithelial basement membranes (BMs) of mouse submandibular gland (SMG) from the onset of branching morphogenesis and became restricted to BMs of epithelial ducts in the adult. To investigate whether the laminin-alpha5 chain plays a role in branching morphogenesis, a set of cell-adhesive peptides from the C-terminal globular domains (LG1-5) was tested for their effects in SMG organ cultures. One peptide, LVLFLNHGH (A5G77f), which represents a sequence located in the connecting loop between strands E and F of LG4. perturbed branching morphogenesis and resulted in irregularities in the contours of epithelial structures, with formation of deep clefts. The data suggest a role for the laminin-alpha5 LG4 module in the development of the duct system, rather than in the bifurcation of epithelial clusters. The epithelial BM of A5G77f-peptide-treated explants was continuous, which was in contrast to our previous finding of impaired epithelial BM assembly in explants treated with the larninin-alpha1 LG4 module peptide, or with a monoclonal antibody against this domain. A5G77f also perturbed in vitro development of lung and kidney. These results suggest a crucial role for the LG4 module of larninin-alpha5 in epithelial morphogenesis that is distinct from that of the laminin-alpha1 LG4. (C) 2003 Elsevier Inc. All rights reserved.},
  author       = {Kadoya, Y and Mochizuki, M and Nomizu, M and Sorokin, Lydia and Yamashina, S},
  issn         = {1095-564X},
  keyword      = {peptide,submandibular gland,organ culture,laminin-alpha 5,laminin-alpha chain,immunohistochemistry,basement membrane,branching morphogenesis},
  language     = {eng},
  number       = {1},
  pages        = {153--164},
  publisher    = {Elsevier},
  series       = {Developmental Biology},
  title        = {Role for laminin-alpha 5 chain LG4 module in epithelial branching morphogenesis},
  url          = {http://dx.doi.org/},
  volume       = {263},
  year         = {2003},
}