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Array Comparative Genomic Hybridization of Keratoacanthomas and Squamous Cell Carcinomas: Different Patterns of Genetic Aberrations Suggest Two Distinct Entities

Li, Jian; Wang, Kai; Gao, Fei; Jensen, Thomas D.; Li, Shengting T.; DeAngelis, Paula M.; Kolvraa, Steen; Proby, Charlotte; Forslund, Ola LU and Bolund, Lars, et al. (2012) In Journal of Investigative Dermatology 132(8). p.2060-2066
Abstract
Keratoacanthoma (KA) is a benign keratinocytic neoplasm that spontaneously regresses after 3-6 months and shares features with squamous cell carcinomas (SCCs). Furthermore, there are reports of KAs that have metastasized, invoking the question of whether KA is a variant of SCC (Hodak et al., 1993). To date, no reported criteria are sensitive enough to discriminate reliably between KA and SCC, and consequently there is a clinical need for discriminating markers. Our previous study analyzed 132 KAs and 29 SCCs and revealed significantly different regions of genomic aberrations using chromosomal comparative genomic hybridization (CGH). In the present study, we applied array CGH to investigate 98 KAs and 22 SCCs from the above samples. The... (More)
Keratoacanthoma (KA) is a benign keratinocytic neoplasm that spontaneously regresses after 3-6 months and shares features with squamous cell carcinomas (SCCs). Furthermore, there are reports of KAs that have metastasized, invoking the question of whether KA is a variant of SCC (Hodak et al., 1993). To date, no reported criteria are sensitive enough to discriminate reliably between KA and SCC, and consequently there is a clinical need for discriminating markers. Our previous study analyzed 132 KAs and 29 SCCs and revealed significantly different regions of genomic aberrations using chromosomal comparative genomic hybridization (CGH). In the present study, we applied array CGH to investigate 98 KAs and 22 SCCs from the above samples. The result shows that all KAs and SCCs have some degree of genetic aberrations. The distribution of numbers of aberrant clones per sample differed significantly between KAs and SCCs (P<0.02), which also demonstrated recurrent aberrations that differed significantly (P<0.001), as illustrated by unsupervised cluster analysis. Classifiers for clinicopathological parameters of KAs were established based on t-test statistics and permutation tests. Tumor size, fibrosis, and inflammation, which are related to the developmental stages of KAs, showed significant (t-test, permutation test) associations with aberrations of selected genomic regions. This suggests chromosomal instability during the whole life cycle of KAs. (Less)
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published
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Journal of Investigative Dermatology
volume
132
issue
8
pages
2060 - 2066
publisher
Nature Publishing Group
external identifiers
  • wos:000306408100018
  • scopus:84863990306
ISSN
1523-1747
DOI
10.1038/jid.2012.104
language
English
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yes
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56e47593-546c-4dfe-ba92-2a1ae9c5006c (old id 2994924)
date added to LUP
2012-09-03 07:18:03
date last changed
2017-10-08 03:00:29
@article{56e47593-546c-4dfe-ba92-2a1ae9c5006c,
  abstract     = {Keratoacanthoma (KA) is a benign keratinocytic neoplasm that spontaneously regresses after 3-6 months and shares features with squamous cell carcinomas (SCCs). Furthermore, there are reports of KAs that have metastasized, invoking the question of whether KA is a variant of SCC (Hodak et al., 1993). To date, no reported criteria are sensitive enough to discriminate reliably between KA and SCC, and consequently there is a clinical need for discriminating markers. Our previous study analyzed 132 KAs and 29 SCCs and revealed significantly different regions of genomic aberrations using chromosomal comparative genomic hybridization (CGH). In the present study, we applied array CGH to investigate 98 KAs and 22 SCCs from the above samples. The result shows that all KAs and SCCs have some degree of genetic aberrations. The distribution of numbers of aberrant clones per sample differed significantly between KAs and SCCs (P&lt;0.02), which also demonstrated recurrent aberrations that differed significantly (P&lt;0.001), as illustrated by unsupervised cluster analysis. Classifiers for clinicopathological parameters of KAs were established based on t-test statistics and permutation tests. Tumor size, fibrosis, and inflammation, which are related to the developmental stages of KAs, showed significant (t-test, permutation test) associations with aberrations of selected genomic regions. This suggests chromosomal instability during the whole life cycle of KAs.},
  author       = {Li, Jian and Wang, Kai and Gao, Fei and Jensen, Thomas D. and Li, Shengting T. and DeAngelis, Paula M. and Kolvraa, Steen and Proby, Charlotte and Forslund, Ola and Bolund, Lars and Clausen, Ole Petter F.},
  issn         = {1523-1747},
  language     = {eng},
  number       = {8},
  pages        = {2060--2066},
  publisher    = {Nature Publishing Group},
  series       = {Journal of Investigative Dermatology},
  title        = {Array Comparative Genomic Hybridization of Keratoacanthomas and Squamous Cell Carcinomas: Different Patterns of Genetic Aberrations Suggest Two Distinct Entities},
  url          = {http://dx.doi.org/10.1038/jid.2012.104},
  volume       = {132},
  year         = {2012},
}