Larval growth rate is not a major determinant of adult wing shape and eyespot size in the seasonally polyphenic butterfly Melanitis leda
(2024) In PeerJ 12(10).- Abstract
Background: Insects often show adaptive phenotypic plasticity where environmental cues during early stages are used to produce a phenotype that matches the environment experienced by adults. Many tropical satyrine butterflies (Nymphalidae: Satyrinae) are seasonally polyphenic and produce distinct wet- and dry-season form adults, providing tight environment-phenotype matching in seasonal environments. In studied Mycalesina butterflies, dry-season forms can be induced in the laboratory by growing larvae at low temperatures or on poor food quality. Since both these factors also tend to reduce larval growth rate, larval growth rate may be an internal cue that translates the environmental cues into the expression of phenotypes. If this is... (More)
Background: Insects often show adaptive phenotypic plasticity where environmental cues during early stages are used to produce a phenotype that matches the environment experienced by adults. Many tropical satyrine butterflies (Nymphalidae: Satyrinae) are seasonally polyphenic and produce distinct wet- and dry-season form adults, providing tight environment-phenotype matching in seasonal environments. In studied Mycalesina butterflies, dry-season forms can be induced in the laboratory by growing larvae at low temperatures or on poor food quality. Since both these factors also tend to reduce larval growth rate, larval growth rate may be an internal cue that translates the environmental cues into the expression of phenotypes. If this is the case, we predict that slower-growing larvae would be more likely to develop a dry-season phenotype. Methods: We performed the first experimental study on seasonal polyphenism of a butterfly in the tribe Melanitini. We measured both larval growth rate and adult phenotype (eyespot size and wing shape) of common evening brown butterflies (Melanitis leda), reared at various temperatures and on various host-plant species. We constructed provisional reaction norms, and tested the hypothesis that growth rate mediates between external cues and adult phenotype. Results: Reaction norms were similar to those found in Mycalesina butterflies. We found that both among and within treatments, larvae with lower growth rates (low temperature, particular host plants) were more likely to develop dry-season phenotypes (small eyespots, falcate wing tips). However, among temperature treatments, similar growth rates could lead to very different wing phenotypes, and within treatments the relationships were weak. Moreover, males and females responded differently, and eyespot size and wing shape were not strongly correlated with each other. Overall, larval growth rate seems to be weakly related to eyespot size and wing shape, indicating that seasonal plasticity in M. leda is primarily mediated by other mechanisms.
(Less)
- author
- Molleman, Freerk ; Moore, M. Elizabeth ; Halali, Sridhar LU ; Kodandaramaiah, Ullasa ; Halali, Dheeraj ; van Bergen, Erik ; Brakefield, Paul M. and Oostra, Vicencio
- organization
- publishing date
- 2024
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Climate, Development time, Eyespots, Growth rate, Host plant, Humidity, Temperature, Wing shape
- in
- PeerJ
- volume
- 12
- issue
- 10
- article number
- e18295
- publisher
- PeerJ
- external identifiers
-
- pmid:39430562
- scopus:85206848626
- ISSN
- 2167-8359
- DOI
- 10.7717/peerj.18295
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2024 PeerJ Inc.. All rights reserved.
- id
- 29c4a7dc-ab7c-4ecc-b9fe-a6598b4f5216
- date added to LUP
- 2024-12-18 17:51:30
- date last changed
- 2025-07-03 10:09:39
@article{29c4a7dc-ab7c-4ecc-b9fe-a6598b4f5216,
abstract = {{<p>Background: Insects often show adaptive phenotypic plasticity where environmental cues during early stages are used to produce a phenotype that matches the environment experienced by adults. Many tropical satyrine butterflies (Nymphalidae: Satyrinae) are seasonally polyphenic and produce distinct wet- and dry-season form adults, providing tight environment-phenotype matching in seasonal environments. In studied Mycalesina butterflies, dry-season forms can be induced in the laboratory by growing larvae at low temperatures or on poor food quality. Since both these factors also tend to reduce larval growth rate, larval growth rate may be an internal cue that translates the environmental cues into the expression of phenotypes. If this is the case, we predict that slower-growing larvae would be more likely to develop a dry-season phenotype. Methods: We performed the first experimental study on seasonal polyphenism of a butterfly in the tribe Melanitini. We measured both larval growth rate and adult phenotype (eyespot size and wing shape) of common evening brown butterflies (Melanitis leda), reared at various temperatures and on various host-plant species. We constructed provisional reaction norms, and tested the hypothesis that growth rate mediates between external cues and adult phenotype. Results: Reaction norms were similar to those found in Mycalesina butterflies. We found that both among and within treatments, larvae with lower growth rates (low temperature, particular host plants) were more likely to develop dry-season phenotypes (small eyespots, falcate wing tips). However, among temperature treatments, similar growth rates could lead to very different wing phenotypes, and within treatments the relationships were weak. Moreover, males and females responded differently, and eyespot size and wing shape were not strongly correlated with each other. Overall, larval growth rate seems to be weakly related to eyespot size and wing shape, indicating that seasonal plasticity in M. leda is primarily mediated by other mechanisms.</p>}},
author = {{Molleman, Freerk and Moore, M. Elizabeth and Halali, Sridhar and Kodandaramaiah, Ullasa and Halali, Dheeraj and van Bergen, Erik and Brakefield, Paul M. and Oostra, Vicencio}},
issn = {{2167-8359}},
keywords = {{Climate; Development time; Eyespots; Growth rate; Host plant; Humidity; Temperature; Wing shape}},
language = {{eng}},
number = {{10}},
publisher = {{PeerJ}},
series = {{PeerJ}},
title = {{Larval growth rate is not a major determinant of adult wing shape and eyespot size in the seasonally polyphenic butterfly Melanitis leda}},
url = {{http://dx.doi.org/10.7717/peerj.18295}},
doi = {{10.7717/peerj.18295}},
volume = {{12}},
year = {{2024}},
}