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A fibrin biofilm covers blood clots and protects from microbial invasion

Macrae, Fraser L. ; Duval, Cédric ; Papareddy, Praveen LU ; Baker, Stephen R. ; Yuldasheva, Nadira ; Kearney, Katherine J. ; McPherson, Helen R. ; Asquith, Nathan ; Konings, Joke and Casini, Alessandro , et al. (2018) In Journal of Clinical Investigation 128(8). p.3356-3368
Abstract

Hemostasis requires conversion of fibrinogen to fibrin fibers that generate a characteristic network, interact with blood cells, and initiate tissue repair. The fibrin network is porous and highly permeable, but the spatial arrangement of the external clot face is unknown. Here we show that fibrin transitioned to the blood-air interface through Langmuir film formation, producing a protective film confining clots in human and mouse models. We demonstrated that only fibrin is required for formation of the film, and that it occurred in vitro and in vivo. The fibrin film connected to the underlying clot network through tethering fibers. It was digested by plasmin, and formation of the film was prevented with surfactants. Functionally, the... (More)

Hemostasis requires conversion of fibrinogen to fibrin fibers that generate a characteristic network, interact with blood cells, and initiate tissue repair. The fibrin network is porous and highly permeable, but the spatial arrangement of the external clot face is unknown. Here we show that fibrin transitioned to the blood-air interface through Langmuir film formation, producing a protective film confining clots in human and mouse models. We demonstrated that only fibrin is required for formation of the film, and that it occurred in vitro and in vivo. The fibrin film connected to the underlying clot network through tethering fibers. It was digested by plasmin, and formation of the film was prevented with surfactants. Functionally, the film retained blood cells and protected against penetration by bacterial pathogens in a murine model of dermal infection. Our data show a remarkable aspect of blood clotting in which fibrin forms a protective film covering the external surface of the clot, defending the organism against microbial invasion.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Investigation
volume
128
issue
8
pages
13 pages
publisher
Am Soc Clin Investig
external identifiers
  • pmid:29723163
  • scopus:85051248986
ISSN
0021-9738
DOI
10.1172/JCI98734
language
English
LU publication?
yes
id
29cece23-167e-4b55-8a17-7e77fd47a9eb
date added to LUP
2018-09-11 08:58:41
date last changed
2020-11-24 02:41:24
@article{29cece23-167e-4b55-8a17-7e77fd47a9eb,
  abstract     = {<p>Hemostasis requires conversion of fibrinogen to fibrin fibers that generate a characteristic network, interact with blood cells, and initiate tissue repair. The fibrin network is porous and highly permeable, but the spatial arrangement of the external clot face is unknown. Here we show that fibrin transitioned to the blood-air interface through Langmuir film formation, producing a protective film confining clots in human and mouse models. We demonstrated that only fibrin is required for formation of the film, and that it occurred in vitro and in vivo. The fibrin film connected to the underlying clot network through tethering fibers. It was digested by plasmin, and formation of the film was prevented with surfactants. Functionally, the film retained blood cells and protected against penetration by bacterial pathogens in a murine model of dermal infection. Our data show a remarkable aspect of blood clotting in which fibrin forms a protective film covering the external surface of the clot, defending the organism against microbial invasion.</p>},
  author       = {Macrae, Fraser L. and Duval, Cédric and Papareddy, Praveen and Baker, Stephen R. and Yuldasheva, Nadira and Kearney, Katherine J. and McPherson, Helen R. and Asquith, Nathan and Konings, Joke and Casini, Alessandro and Degen, Jay L. and Connell, Simon D. and Philippou, Helen and Wolberg, Alisa S. and Herwald, Heiko and Ariëns, Robert A.S.},
  issn         = {0021-9738},
  language     = {eng},
  month        = {08},
  number       = {8},
  pages        = {3356--3368},
  publisher    = {Am Soc Clin Investig},
  series       = {Journal of Clinical Investigation},
  title        = {A fibrin biofilm covers blood clots and protects from microbial invasion},
  url          = {http://dx.doi.org/10.1172/JCI98734},
  doi          = {10.1172/JCI98734},
  volume       = {128},
  year         = {2018},
}