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Oral Challenges with Four Apple Cultivars Result in Significant Differences in Oral Allergy Symptoms

Nybom, Hilde ; Cervin-Hoberg, Charlotte LU and Andersson, Morgan LU (2013) In International Archives of Allergy and Immunology 161(3). p.258-264
Abstract
Background: We analyzed the hypoallergenic potential of a recently bred apple selection with unusually low content of Mal d 1, using an oral challenge model with three additional apple cultivars for comparison. Methods: Sixty-six birch pollen-allergic individuals with a history of oral allergy syndrome after apple intake were subjected to a double-blind oral provocation with two apple cultivars (B:0654 and 'Discovery'). Thirteen also tested two other apple cultivars ('Ingrid Marie' and 'Gloster'). Three doses were given consecutively, 30 min apart: 10 g without peel, and 10 and 50 g with peel. A final assessment was conducted 30 min after the last intake. Oral symptoms were graded from 0 to 5. Total oral symptom score (TOS) included all... (More)
Background: We analyzed the hypoallergenic potential of a recently bred apple selection with unusually low content of Mal d 1, using an oral challenge model with three additional apple cultivars for comparison. Methods: Sixty-six birch pollen-allergic individuals with a history of oral allergy syndrome after apple intake were subjected to a double-blind oral provocation with two apple cultivars (B:0654 and 'Discovery'). Thirteen also tested two other apple cultivars ('Ingrid Marie' and 'Gloster'). Three doses were given consecutively, 30 min apart: 10 g without peel, and 10 and 50 g with peel. A final assessment was conducted 30 min after the last intake. Oral symptoms were graded from 0 to 5. Total oral symptom score (TOS) included all scores for each cultivar at all time points. Results: B:0654 induced significantly higher TOS than 'Discovery' when tested by 66 individuals, in spite of its lower Mal d 1 content. TOS values were higher in females and increased with increasing age of the individuals when challenged with 'Discovery'. Among the 13 individuals who tested all four cultivars, B:0654 produced a higher score after the second dose compared to 'Ingrid Marie'. This was also the case after the third dose compared to 'Ingrid Marie' and 'Gloster', and again 30 min after the last intake compared to each of the other three cultivars, as well as a higher TOS compared to each of the other three cultivars (all p <0.01). Conclusions: Our test was safe and well tolerated, and produced significant differences among the apple cultivars. Contrary to expectations, B:0654 was less well tolerated than the other three cultivars. Copyright (c) 2013 S. Karger AG, Basel (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Apple, Birch pollen, Mal d 1, Oral allergy syndrome, Oral provocation
in
International Archives of Allergy and Immunology
volume
161
issue
3
pages
258 - 264
publisher
Karger
external identifiers
  • wos:000320637900010
  • scopus:84875659073
  • pmid:23548468
ISSN
1423-0097
DOI
10.1159/000345954
language
English
LU publication?
yes
id
29e1aec5-b564-47a8-8557-cd092641ca18 (old id 3987150)
date added to LUP
2016-04-01 10:27:57
date last changed
2020-03-18 01:17:17
@article{29e1aec5-b564-47a8-8557-cd092641ca18,
  abstract     = {Background: We analyzed the hypoallergenic potential of a recently bred apple selection with unusually low content of Mal d 1, using an oral challenge model with three additional apple cultivars for comparison. Methods: Sixty-six birch pollen-allergic individuals with a history of oral allergy syndrome after apple intake were subjected to a double-blind oral provocation with two apple cultivars (B:0654 and 'Discovery'). Thirteen also tested two other apple cultivars ('Ingrid Marie' and 'Gloster'). Three doses were given consecutively, 30 min apart: 10 g without peel, and 10 and 50 g with peel. A final assessment was conducted 30 min after the last intake. Oral symptoms were graded from 0 to 5. Total oral symptom score (TOS) included all scores for each cultivar at all time points. Results: B:0654 induced significantly higher TOS than 'Discovery' when tested by 66 individuals, in spite of its lower Mal d 1 content. TOS values were higher in females and increased with increasing age of the individuals when challenged with 'Discovery'. Among the 13 individuals who tested all four cultivars, B:0654 produced a higher score after the second dose compared to 'Ingrid Marie'. This was also the case after the third dose compared to 'Ingrid Marie' and 'Gloster', and again 30 min after the last intake compared to each of the other three cultivars, as well as a higher TOS compared to each of the other three cultivars (all p &lt;0.01). Conclusions: Our test was safe and well tolerated, and produced significant differences among the apple cultivars. Contrary to expectations, B:0654 was less well tolerated than the other three cultivars. Copyright (c) 2013 S. Karger AG, Basel},
  author       = {Nybom, Hilde and Cervin-Hoberg, Charlotte and Andersson, Morgan},
  issn         = {1423-0097},
  language     = {eng},
  number       = {3},
  pages        = {258--264},
  publisher    = {Karger},
  series       = {International Archives of Allergy and Immunology},
  title        = {Oral Challenges with Four Apple Cultivars Result in Significant Differences in Oral Allergy Symptoms},
  url          = {http://dx.doi.org/10.1159/000345954},
  doi          = {10.1159/000345954},
  volume       = {161},
  year         = {2013},
}