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Time-resolved quantitative proteome analysis of in vivo intestinal development

Hansson, Jenny LU ; Panchaud, Alexandre; Favre, Laurent; Bosco, Nabil; Mansourian, Robert; Benyacoub, Jalil; Blum, Stephanie; Jensen, Ole N and Kussmann, Martin (2011) In Molecular & Cellular Proteomics 10(3). p.110-005231
Abstract

Postnatal intestinal development is a very dynamic process characterized by substantial morphological changes that coincide with functional adaption to the nutritional change from a diet rich in fat (milk) to a diet rich in carbohydrates on from weaning. Time-resolved studies of intestinal development have so far been limited to investigation at the transcription level or to single or few proteins at a time. In the present study, we elucidate proteomic changes of primary intestinal epithelial cells from jejunum during early suckling (1-7 days of age), middle suckling (7-14 days), and weaning period (14-35 days) in mice, using a label-free proteomics approach. We show differential expression of 520 proteins during intestinal development... (More)

Postnatal intestinal development is a very dynamic process characterized by substantial morphological changes that coincide with functional adaption to the nutritional change from a diet rich in fat (milk) to a diet rich in carbohydrates on from weaning. Time-resolved studies of intestinal development have so far been limited to investigation at the transcription level or to single or few proteins at a time. In the present study, we elucidate proteomic changes of primary intestinal epithelial cells from jejunum during early suckling (1-7 days of age), middle suckling (7-14 days), and weaning period (14-35 days) in mice, using a label-free proteomics approach. We show differential expression of 520 proteins during intestinal development and a pronounced change of the proteome during the middle suckling period and weaning. Proteins involved in several metabolic processes were found differentially expressed along the development. The temporal expression profiles of enzymes of the glycolysis were found to correlate with the increase in carbohydrate uptake at weaning, whereas the abundance changes of proteins involved in fatty acid metabolism as well as lactose metabolism indicated a nondiet driven preparation for the nutritional change at weaning. Further, we report the developmental abundance changes of proteins playing a vital role in the neonatal acquisition of passive immunity. In addition, different isoforms of several proteins were quantified, which may contribute to a better understanding of the roles of the specific isoforms in the small intestine. In summary, we provide a first, time-resolved proteome profile of intestinal epithelial cells along postnatal intestinal development.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Carbohydrate Metabolism, Databases, Protein, Epithelial Cells, Fatty Acids, Glycolysis, Intestinal Absorption, Intestines, Isotope Labeling, Lipid Metabolism, Mice, Mice, Inbred C57BL, Peptides, Proteome, Proteomics, Time Factors
in
Molecular & Cellular Proteomics
volume
10
issue
3
pages
110 - 005231
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • scopus:79953205617
ISSN
1535-9484
DOI
10.1074/mcp.M110.005231
language
English
LU publication?
no
id
2a259551-757c-49b6-82b0-6105b8deaa6d
date added to LUP
2016-08-25 09:54:44
date last changed
2017-04-09 04:48:18
@article{2a259551-757c-49b6-82b0-6105b8deaa6d,
  abstract     = {<p>Postnatal intestinal development is a very dynamic process characterized by substantial morphological changes that coincide with functional adaption to the nutritional change from a diet rich in fat (milk) to a diet rich in carbohydrates on from weaning. Time-resolved studies of intestinal development have so far been limited to investigation at the transcription level or to single or few proteins at a time. In the present study, we elucidate proteomic changes of primary intestinal epithelial cells from jejunum during early suckling (1-7 days of age), middle suckling (7-14 days), and weaning period (14-35 days) in mice, using a label-free proteomics approach. We show differential expression of 520 proteins during intestinal development and a pronounced change of the proteome during the middle suckling period and weaning. Proteins involved in several metabolic processes were found differentially expressed along the development. The temporal expression profiles of enzymes of the glycolysis were found to correlate with the increase in carbohydrate uptake at weaning, whereas the abundance changes of proteins involved in fatty acid metabolism as well as lactose metabolism indicated a nondiet driven preparation for the nutritional change at weaning. Further, we report the developmental abundance changes of proteins playing a vital role in the neonatal acquisition of passive immunity. In addition, different isoforms of several proteins were quantified, which may contribute to a better understanding of the roles of the specific isoforms in the small intestine. In summary, we provide a first, time-resolved proteome profile of intestinal epithelial cells along postnatal intestinal development.</p>},
  author       = {Hansson, Jenny and Panchaud, Alexandre and Favre, Laurent and Bosco, Nabil and Mansourian, Robert and Benyacoub, Jalil and Blum, Stephanie and Jensen, Ole N and Kussmann, Martin},
  issn         = {1535-9484},
  keyword      = {Animals,Carbohydrate Metabolism,Databases, Protein,Epithelial Cells,Fatty Acids,Glycolysis,Intestinal Absorption,Intestines,Isotope Labeling,Lipid Metabolism,Mice,Mice, Inbred C57BL,Peptides,Proteome,Proteomics,Time Factors},
  language     = {eng},
  number       = {3},
  pages        = {110--005231},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular & Cellular Proteomics},
  title        = {Time-resolved quantitative proteome analysis of in vivo intestinal development},
  url          = {http://dx.doi.org/10.1074/mcp.M110.005231},
  volume       = {10},
  year         = {2011},
}