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Processing and secretion of rat alpha 1-microglobulin-bikunin expressed in eukaryotic cell lines

Bratt, T ; Cedervall, T LU and Akerström, B LU (1994) In FEBS Letters 354(1). p.57-61
Abstract

The precursor protein alpha 1-microglobulin-bikunin was cleaved to the same degree whether expressed in CHO cells or in mutated CHO cells, RPE.40 cells, suggested to lack a functional form of the intracellular protease furin. Thus, alpha 1-microglobulin-bikunin probably is not cleaved in vivo by furin. However, simultaneous overexpression of the precursor and furin in COS, CHO and RPE.40 cells increased the cleavage, suggesting that compartmentalisation and concentrations of protease and precursor are important for the cleavage, besides the in vitro specificity. Expression of alpha 1-microglobulin and bikunin alone gave different protein patterns of SDS-PAGE as compared to expression of the precursor and subsequent cleavage, suggesting... (More)

The precursor protein alpha 1-microglobulin-bikunin was cleaved to the same degree whether expressed in CHO cells or in mutated CHO cells, RPE.40 cells, suggested to lack a functional form of the intracellular protease furin. Thus, alpha 1-microglobulin-bikunin probably is not cleaved in vivo by furin. However, simultaneous overexpression of the precursor and furin in COS, CHO and RPE.40 cells increased the cleavage, suggesting that compartmentalisation and concentrations of protease and precursor are important for the cleavage, besides the in vitro specificity. Expression of alpha 1-microglobulin and bikunin alone gave different protein patterns of SDS-PAGE as compared to expression of the precursor and subsequent cleavage, suggesting that the precursor protein is important for the post-translational handling of alpha 1-microglobulin and bikunin.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alpha-Globulins/chemistry, Animals, Base Sequence, CHO Cells, Cricetinae, Furin, Gene Expression, Glycoproteins/chemistry, Membrane Glycoproteins, Molecular Sequence Data, Molecular Weight, Protease Inhibitors/metabolism, Protein Precursors/genetics, Protein Processing, Post-Translational/physiology, Rats, Subtilisins/genetics, Transfection, Trypsin Inhibitor, Kunitz Soybean
in
FEBS Letters
volume
354
issue
1
pages
57 - 61
publisher
Wiley-Blackwell
external identifiers
  • pmid:7525349
  • scopus:0027992751
ISSN
0014-5793
DOI
10.1016/0014-5793(94)01087-0
language
English
LU publication?
yes
id
2a9d0aa5-1407-4a2b-8b09-c40bdbca97f8
date added to LUP
2019-05-22 10:21:24
date last changed
2024-06-26 17:34:09
@article{2a9d0aa5-1407-4a2b-8b09-c40bdbca97f8,
  abstract     = {{<p>The precursor protein alpha 1-microglobulin-bikunin was cleaved to the same degree whether expressed in CHO cells or in mutated CHO cells, RPE.40 cells, suggested to lack a functional form of the intracellular protease furin. Thus, alpha 1-microglobulin-bikunin probably is not cleaved in vivo by furin. However, simultaneous overexpression of the precursor and furin in COS, CHO and RPE.40 cells increased the cleavage, suggesting that compartmentalisation and concentrations of protease and precursor are important for the cleavage, besides the in vitro specificity. Expression of alpha 1-microglobulin and bikunin alone gave different protein patterns of SDS-PAGE as compared to expression of the precursor and subsequent cleavage, suggesting that the precursor protein is important for the post-translational handling of alpha 1-microglobulin and bikunin.</p>}},
  author       = {{Bratt, T and Cedervall, T and Akerström, B}},
  issn         = {{0014-5793}},
  keywords     = {{Alpha-Globulins/chemistry; Animals; Base Sequence; CHO Cells; Cricetinae; Furin; Gene Expression; Glycoproteins/chemistry; Membrane Glycoproteins; Molecular Sequence Data; Molecular Weight; Protease Inhibitors/metabolism; Protein Precursors/genetics; Protein Processing, Post-Translational/physiology; Rats; Subtilisins/genetics; Transfection; Trypsin Inhibitor, Kunitz Soybean}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{1}},
  pages        = {{57--61}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Processing and secretion of rat alpha 1-microglobulin-bikunin expressed in eukaryotic cell lines}},
  url          = {{http://dx.doi.org/10.1016/0014-5793(94)01087-0}},
  doi          = {{10.1016/0014-5793(94)01087-0}},
  volume       = {{354}},
  year         = {{1994}},
}