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Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin

Lilja, H LU orcid ; Christensson, A LU ; Dahlén, U ; Matikainen, M T ; Nilsson, O ; Pettersson, K and Lövgren, T (1991) In Clinical Chemistry 37(9). p.25-1618
Abstract

Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active... (More)

Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active proteinase but most poorly detected PSA complexed to alpha 1-antichymotrypsin; and assay C, which was specific for PSA complexed to alpha 1-antichymotrypsin. We used the three assays to measure PSA immunoreactivity in 64 patients' sera and in the effluent after gel chromatography of sera from four patients. This identified an 80- to 90-kDa complex between PSA and alpha 1-antichymotrypsin as the predominant fraction of the PSA immunoreactivity in blood plasma; an immunoreactive 25- to 40-kDa compound was the minor fraction.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Antibodies, Monoclonal, Antigens, Neoplasm/blood, Biomarkers, Tumor/blood, Chromatography, Gel, Electrophoresis, Agar Gel, Electrophoresis, Polyacrylamide Gel, Epitopes/analysis, Female, Humans, Male, Mice, Mice, Inbred BALB C, Prostate-Specific Antigen, alpha 1-Antichymotrypsin/immunology
in
Clinical Chemistry
volume
37
issue
9
pages
8 pages
publisher
American Association for Clinical Chemistry
external identifiers
  • scopus:0026077430
  • pmid:1716536
ISSN
0009-9147
DOI
10.1093/clinchem/37.9.1618
language
English
LU publication?
yes
id
2ac14579-6d2f-45c8-9f04-84bf770cc03d
date added to LUP
2019-05-16 14:08:42
date last changed
2024-09-04 18:57:54
@article{2ac14579-6d2f-45c8-9f04-84bf770cc03d,
  abstract     = {{<p>Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active proteinase but most poorly detected PSA complexed to alpha 1-antichymotrypsin; and assay C, which was specific for PSA complexed to alpha 1-antichymotrypsin. We used the three assays to measure PSA immunoreactivity in 64 patients' sera and in the effluent after gel chromatography of sera from four patients. This identified an 80- to 90-kDa complex between PSA and alpha 1-antichymotrypsin as the predominant fraction of the PSA immunoreactivity in blood plasma; an immunoreactive 25- to 40-kDa compound was the minor fraction.</p>}},
  author       = {{Lilja, H and Christensson, A and Dahlén, U and Matikainen, M T and Nilsson, O and Pettersson, K and Lövgren, T}},
  issn         = {{0009-9147}},
  keywords     = {{Animals; Antibodies, Monoclonal; Antigens, Neoplasm/blood; Biomarkers, Tumor/blood; Chromatography, Gel; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Epitopes/analysis; Female; Humans; Male; Mice; Mice, Inbred BALB C; Prostate-Specific Antigen; alpha 1-Antichymotrypsin/immunology}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{25--1618}},
  publisher    = {{American Association for Clinical Chemistry}},
  series       = {{Clinical Chemistry}},
  title        = {{Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin}},
  url          = {{http://dx.doi.org/10.1093/clinchem/37.9.1618}},
  doi          = {{10.1093/clinchem/37.9.1618}},
  volume       = {{37}},
  year         = {{1991}},
}