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Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin

Lilja, H LU ; Christensson, A LU ; Dahlén, U ; Matikainen, M T ; Nilsson, O ; Pettersson, K and Lövgren, T (1991) In Clinical Chemistry 37(9). p.25-1618
Abstract

Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active... (More)

Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active proteinase but most poorly detected PSA complexed to alpha 1-antichymotrypsin; and assay C, which was specific for PSA complexed to alpha 1-antichymotrypsin. We used the three assays to measure PSA immunoreactivity in 64 patients' sera and in the effluent after gel chromatography of sera from four patients. This identified an 80- to 90-kDa complex between PSA and alpha 1-antichymotrypsin as the predominant fraction of the PSA immunoreactivity in blood plasma; an immunoreactive 25- to 40-kDa compound was the minor fraction.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Antibodies, Monoclonal, Antigens, Neoplasm/blood, Biomarkers, Tumor/blood, Chromatography, Gel, Electrophoresis, Agar Gel, Electrophoresis, Polyacrylamide Gel, Epitopes/analysis, Female, Humans, Male, Mice, Mice, Inbred BALB C, Prostate-Specific Antigen, alpha 1-Antichymotrypsin/immunology
in
Clinical Chemistry
volume
37
issue
9
pages
8 pages
publisher
American Association for Clinical Chemistry
external identifiers
  • scopus:0026077430
  • pmid:1716536
ISSN
0009-9147
language
English
LU publication?
no
id
2ac14579-6d2f-45c8-9f04-84bf770cc03d
alternative location
http://clinchem.aaccjnls.org/content/37/9/1618/tab-article-info
date added to LUP
2019-05-16 14:08:42
date last changed
2019-11-24 05:34:58
@article{2ac14579-6d2f-45c8-9f04-84bf770cc03d,
  abstract     = {<p>Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active proteinase but most poorly detected PSA complexed to alpha 1-antichymotrypsin; and assay C, which was specific for PSA complexed to alpha 1-antichymotrypsin. We used the three assays to measure PSA immunoreactivity in 64 patients' sera and in the effluent after gel chromatography of sera from four patients. This identified an 80- to 90-kDa complex between PSA and alpha 1-antichymotrypsin as the predominant fraction of the PSA immunoreactivity in blood plasma; an immunoreactive 25- to 40-kDa compound was the minor fraction.</p>},
  author       = {Lilja, H and Christensson, A and Dahlén, U and Matikainen, M T and Nilsson, O and Pettersson, K and Lövgren, T},
  issn         = {0009-9147},
  language     = {eng},
  number       = {9},
  pages        = {25--1618},
  publisher    = {American Association for Clinical Chemistry},
  series       = {Clinical Chemistry},
  title        = {Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin},
  url          = {http://clinchem.aaccjnls.org/content/37/9/1618/tab-article-info},
  volume       = {37},
  year         = {1991},
}