Protease-, pectinase-and amylase-producing bacteria from a Kenyan soda lake
(2018) In Open Biotechnology Journal 12. p.33-45- Abstract
Background: Alkaline enzymes are stable biocatalysts with potential applications in industrial technologies that offer high quality products. Objective: The growing demand for alkaline enzymes in industry has enhanced the search for microorganisms that produce these enzymes. Methods: Eighteen bacterial isolates from Lake Bogoria, Kenya, were screened for alkaline proteases, pectinases and amylases; characterized and subjected to quantitative analysis of the enzymes they produced. Results: The screening analysis ranked 14, 16 and 18 of the bacterial isolates as potent producers of alkaline proteases, pectinases and amylases, respectively. The isolates were classified into two groups: Group 1 (16 isolates) were facultatively alkaliphilic... (More)
Background: Alkaline enzymes are stable biocatalysts with potential applications in industrial technologies that offer high quality products. Objective: The growing demand for alkaline enzymes in industry has enhanced the search for microorganisms that produce these enzymes. Methods: Eighteen bacterial isolates from Lake Bogoria, Kenya, were screened for alkaline proteases, pectinases and amylases; characterized and subjected to quantitative analysis of the enzymes they produced. Results: The screening analysis ranked 14, 16 and 18 of the bacterial isolates as potent producers of alkaline proteases, pectinases and amylases, respectively. The isolates were classified into two groups: Group 1 (16 isolates) were facultatively alkaliphilic B. halodurans while group 2 (2 isolates) were obligately alkaliphilic B. pseudofirmus. Further analysis revealed that group 1 isolates were divided into two sub-groups, with sub-group I (4 isolates) being a phenotypic variant sub-population of sub-group II (12 isolates). Variation between the two populations was also observed in their enzymatic production profiles e.g. sub-group I isolates did not produce alkaline proteolytic enzymes while those in sub-group II did so (0.01-0.36 U/ml). Furthermore, they produced higher levels of the alkaline pectinolytic enzyme polygalacturonase (0.12-0.46 U/ml) compared to sub-group II isolates (0.05-0.10 U/ml), which also produced another pectinolytic enzyme-pectate lyase (0.01 U/ml). No clear distinction was however, observed in the production profiles of alkaline amylolytic enzymes by the isolates in the two sub-populations [0.20-0.40 U/ml (amylases), 0.24-0.68 U/ml (pullulanases) and 0.01-0.03 U/ml (cyclodextrin glycosyl transferases)]. On the other hand, group 2 isolates were phenotypically identical to one another and also produced similar amounts of proteolytic (0.38, 0.40 U/ml) and amylolytic [amylases (0.06, 0.1 U/ml), pullulanases (0.06, 0.09 U/ml) and cyclodextrin glycosyl transferases (0.01, 0.02 U/ml)] enzymes. Conclusion: The facultatively alkaliphilic B. halodurans and obligately alkaliphilic B. pseudofirmus isolates are attractive biotechnological sources of industrially important alkaline enzymes.
(Less)
- author
- Oluoch, Kevin Raymond LU ; Okanya, Patrick Wafula ; Hatti-Kaul, Rajni LU ; Mattiasson, Bo LU and Mulaa, Francis Jakim
- organization
- publishing date
- 2018-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alkaliphiles, Amylases, Bacillus halodurans, Bacillus pseudofirmus, Pectinases, Proteases, Soda lake
- in
- Open Biotechnology Journal
- volume
- 12
- pages
- 13 pages
- publisher
- Bentham Science Publishers
- external identifiers
-
- scopus:85049116834
- ISSN
- 1874-0707
- DOI
- 10.2174/1874070701812010033
- language
- English
- LU publication?
- yes
- id
- 2acb666f-5df9-434c-a710-ef5c491feafc
- date added to LUP
- 2018-07-09 13:44:30
- date last changed
- 2022-04-25 08:18:35
@article{2acb666f-5df9-434c-a710-ef5c491feafc,
abstract = {{<p>Background: Alkaline enzymes are stable biocatalysts with potential applications in industrial technologies that offer high quality products. Objective: The growing demand for alkaline enzymes in industry has enhanced the search for microorganisms that produce these enzymes. Methods: Eighteen bacterial isolates from Lake Bogoria, Kenya, were screened for alkaline proteases, pectinases and amylases; characterized and subjected to quantitative analysis of the enzymes they produced. Results: The screening analysis ranked 14, 16 and 18 of the bacterial isolates as potent producers of alkaline proteases, pectinases and amylases, respectively. The isolates were classified into two groups: Group 1 (16 isolates) were facultatively alkaliphilic B. halodurans while group 2 (2 isolates) were obligately alkaliphilic B. pseudofirmus. Further analysis revealed that group 1 isolates were divided into two sub-groups, with sub-group I (4 isolates) being a phenotypic variant sub-population of sub-group II (12 isolates). Variation between the two populations was also observed in their enzymatic production profiles e.g. sub-group I isolates did not produce alkaline proteolytic enzymes while those in sub-group II did so (0.01-0.36 U/ml). Furthermore, they produced higher levels of the alkaline pectinolytic enzyme polygalacturonase (0.12-0.46 U/ml) compared to sub-group II isolates (0.05-0.10 U/ml), which also produced another pectinolytic enzyme-pectate lyase (0.01 U/ml). No clear distinction was however, observed in the production profiles of alkaline amylolytic enzymes by the isolates in the two sub-populations [0.20-0.40 U/ml (amylases), 0.24-0.68 U/ml (pullulanases) and 0.01-0.03 U/ml (cyclodextrin glycosyl transferases)]. On the other hand, group 2 isolates were phenotypically identical to one another and also produced similar amounts of proteolytic (0.38, 0.40 U/ml) and amylolytic [amylases (0.06, 0.1 U/ml), pullulanases (0.06, 0.09 U/ml) and cyclodextrin glycosyl transferases (0.01, 0.02 U/ml)] enzymes. Conclusion: The facultatively alkaliphilic B. halodurans and obligately alkaliphilic B. pseudofirmus isolates are attractive biotechnological sources of industrially important alkaline enzymes.</p>}},
author = {{Oluoch, Kevin Raymond and Okanya, Patrick Wafula and Hatti-Kaul, Rajni and Mattiasson, Bo and Mulaa, Francis Jakim}},
issn = {{1874-0707}},
keywords = {{Alkaliphiles; Amylases; Bacillus halodurans; Bacillus pseudofirmus; Pectinases; Proteases; Soda lake}},
language = {{eng}},
month = {{01}},
pages = {{33--45}},
publisher = {{Bentham Science Publishers}},
series = {{Open Biotechnology Journal}},
title = {{Protease-, pectinase-and amylase-producing bacteria from a Kenyan soda lake}},
url = {{http://dx.doi.org/10.2174/1874070701812010033}},
doi = {{10.2174/1874070701812010033}},
volume = {{12}},
year = {{2018}},
}