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Behavior of silk protein at the air-water interface

Yang, Yuhong ; Dicko, Cedric LU orcid ; Bain, Colin D. ; Gong, Zuguang ; Jacobs, Robert M. J. ; Shao, Zhengzhong ; Terry, Ann E. and Vollrath, Fritz (2012) In Soft Matter 8(37). p.9705-9712
Abstract
The inability to link silk biomacromolecules' activity to their self-assembly and further fiber formation has limited a true implementation of a silk biotechnology. In this paper, we describe the application of video-enhanced drop shape analysis and interfacial shear rheological measurement to characterize the dynamic surface activity and interfacial interactions, as well as molecular structure within the interfacial layer of silk proteins. Quantitative analysis of the dynamic surface tension highlights two major mechanisms: (i) a mixed model at low concentration, and, (ii) a diffusion limited model at higher concentration. Once regenerated silk fibroin (RSF) is adsorbed at the air-water interface, interfacial gel-like structures are... (More)
The inability to link silk biomacromolecules' activity to their self-assembly and further fiber formation has limited a true implementation of a silk biotechnology. In this paper, we describe the application of video-enhanced drop shape analysis and interfacial shear rheological measurement to characterize the dynamic surface activity and interfacial interactions, as well as molecular structure within the interfacial layer of silk proteins. Quantitative analysis of the dynamic surface tension highlights two major mechanisms: (i) a mixed model at low concentration, and, (ii) a diffusion limited model at higher concentration. Once regenerated silk fibroin (RSF) is adsorbed at the air-water interface, interfacial gel-like structures are formed. The interfacial elastic modulus (G') of the adsorbed membranes exhibited a non-monotonic concentration dependence with a local maximum value at 1.0 x 10(-5) g mL(-1), indicating a different surface structure formed in RSF solution. The viscoelastic behavior varies with RSF concentration corresponding to three states of a soft glassy system, i. e. below, above and near the glass transition. A structural model for RSF adsorbed layers at the air-water interface at different bulk concentrations is suggested. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
8
issue
37
pages
9705 - 9712
publisher
Royal Society of Chemistry
external identifiers
  • wos:000308095700025
  • scopus:84865781903
ISSN
1744-6848
DOI
10.1039/c2sm26054a
language
English
LU publication?
yes
id
2aee0ec7-77c9-41af-a044-b6a18007272f (old id 3191669)
date added to LUP
2016-04-01 14:11:39
date last changed
2022-04-22 01:56:01
@article{2aee0ec7-77c9-41af-a044-b6a18007272f,
  abstract     = {{The inability to link silk biomacromolecules' activity to their self-assembly and further fiber formation has limited a true implementation of a silk biotechnology. In this paper, we describe the application of video-enhanced drop shape analysis and interfacial shear rheological measurement to characterize the dynamic surface activity and interfacial interactions, as well as molecular structure within the interfacial layer of silk proteins. Quantitative analysis of the dynamic surface tension highlights two major mechanisms: (i) a mixed model at low concentration, and, (ii) a diffusion limited model at higher concentration. Once regenerated silk fibroin (RSF) is adsorbed at the air-water interface, interfacial gel-like structures are formed. The interfacial elastic modulus (G') of the adsorbed membranes exhibited a non-monotonic concentration dependence with a local maximum value at 1.0 x 10(-5) g mL(-1), indicating a different surface structure formed in RSF solution. The viscoelastic behavior varies with RSF concentration corresponding to three states of a soft glassy system, i. e. below, above and near the glass transition. A structural model for RSF adsorbed layers at the air-water interface at different bulk concentrations is suggested.}},
  author       = {{Yang, Yuhong and Dicko, Cedric and Bain, Colin D. and Gong, Zuguang and Jacobs, Robert M. J. and Shao, Zhengzhong and Terry, Ann E. and Vollrath, Fritz}},
  issn         = {{1744-6848}},
  language     = {{eng}},
  number       = {{37}},
  pages        = {{9705--9712}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Soft Matter}},
  title        = {{Behavior of silk protein at the air-water interface}},
  url          = {{http://dx.doi.org/10.1039/c2sm26054a}},
  doi          = {{10.1039/c2sm26054a}},
  volume       = {{8}},
  year         = {{2012}},
}