Exploring central and peripheral diversity in antibody evolution.
Persson, Helena LU and Ohlin, Mats LU
(2007)
In Molecular Immunology
44(10).
p.2729-2736
- Abstract
- The antigen-binding site, the paratope, of an antibody can be seen as being composed of a central core and a more peripheral area situated at its rim. Naturally these regions acquire their diversity using different mechanisms and they also have dissimilar roles, as they contribute differently to the binding interaction. Also, antigens of different size utilize these regions differently; while haptens mainly interact with the central core, larger antigens have additional interactions in more peripheral regions. Since haptens do not occupy the entire available paratope we hypothesized that hapten-specific antibodies, as they develop naturally or in the laboratory, have an imprint of the carrier protein they were once selected on. By using... (More)
- The antigen-binding site, the paratope, of an antibody can be seen as being composed of a central core and a more peripheral area situated at its rim. Naturally these regions acquire their diversity using different mechanisms and they also have dissimilar roles, as they contribute differently to the binding interaction. Also, antigens of different size utilize these regions differently; while haptens mainly interact with the central core, larger antigens have additional interactions in more peripheral regions. Since haptens do not occupy the entire available paratope we hypothesized that hapten-specific antibodies, as they develop naturally or in the laboratory, have an imprint of the carrier protein they were once selected on. By using combinatorial library and phage display technologies on a hapten-specific antibody we were able to demonstrate that a peripheral carrier imprint indeed exists. We further show that such an imprint can act as a seed in the evolution of binders that recognize the carrier protein even in the absence of the hapten modification. The observed results provide a plausible mechanism for how haptenization of self-antigens can lead to the development of autoimmunity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/594434
- author
- Persson, Helena
LU
and Ohlin, Mats
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Autoimmunity, Hapten, Antibody library, Antibody evolution, Phage display, Paratope
- in
- Molecular Immunology
- volume
- 44
- issue
- 10
- pages
- 2729 - 2736
- publisher
- Pergamon Press Ltd.
- external identifiers
-
- wos:000245681200025
- scopus:33846927264
- pmid:17204327
- ISSN
- 1872-9142
- DOI
- 10.1016/j.molimm.2006.11.024
- language
- English
- LU publication?
- yes
- id
- 2b769cca-e0a4-47d1-88ee-ba3863eed68b (old id 594434)
- date added to LUP
- 2016-04-01 16:32:42
- date last changed
- 2022-01-28 20:28:33
@article{2b769cca-e0a4-47d1-88ee-ba3863eed68b,
abstract = {{The antigen-binding site, the paratope, of an antibody can be seen as being composed of a central core and a more peripheral area situated at its rim. Naturally these regions acquire their diversity using different mechanisms and they also have dissimilar roles, as they contribute differently to the binding interaction. Also, antigens of different size utilize these regions differently; while haptens mainly interact with the central core, larger antigens have additional interactions in more peripheral regions. Since haptens do not occupy the entire available paratope we hypothesized that hapten-specific antibodies, as they develop naturally or in the laboratory, have an imprint of the carrier protein they were once selected on. By using combinatorial library and phage display technologies on a hapten-specific antibody we were able to demonstrate that a peripheral carrier imprint indeed exists. We further show that such an imprint can act as a seed in the evolution of binders that recognize the carrier protein even in the absence of the hapten modification. The observed results provide a plausible mechanism for how haptenization of self-antigens can lead to the development of autoimmunity.}},
author = {{Persson, Helena and Ohlin, Mats}},
issn = {{1872-9142}},
keywords = {{Autoimmunity; Hapten; Antibody library; Antibody evolution; Phage display; Paratope}},
language = {{eng}},
number = {{10}},
pages = {{2729--2736}},
publisher = {{Pergamon Press Ltd.}},
series = {{Molecular Immunology}},
title = {{Exploring central and peripheral diversity in antibody evolution.}},
url = {{http://dx.doi.org/10.1016/j.molimm.2006.11.024}},
doi = {{10.1016/j.molimm.2006.11.024}},
volume = {{44}},
year = {{2007}},
}