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Mitogen activated protein (MAP) kinase activity is increased in adult mouse superior cervical ganglia during culturing

Svensson, Bodil and Ekström, Per LU (1998) In Journal of Neuroscience Research 52(4). p.453-457
Abstract

We have used adult mouse superior cervical ganglia (SCG) to study the role of mitogen activated protein (MAP) kinase activity during axonal outgrowth in vitro. An initial peak in activity within the first hours of culture was followed by a substantially higher activity after 1 to 2 days, a time when axons were actively growing. The latter peak is probably a result of both higher levels of protein and increased activity. The addition of nerve growth factor stimulated both outgrowth and kinase activity, whereas treating the cultures with the kinase inhibitor PD98059 had an opposite effect. Taken together, the results suggest that activation of the MAP kinase pathway could be involved in the initiation as well as regulation of axonal... (More)

We have used adult mouse superior cervical ganglia (SCG) to study the role of mitogen activated protein (MAP) kinase activity during axonal outgrowth in vitro. An initial peak in activity within the first hours of culture was followed by a substantially higher activity after 1 to 2 days, a time when axons were actively growing. The latter peak is probably a result of both higher levels of protein and increased activity. The addition of nerve growth factor stimulated both outgrowth and kinase activity, whereas treating the cultures with the kinase inhibitor PD98059 had an opposite effect. Taken together, the results suggest that activation of the MAP kinase pathway could be involved in the initiation as well as regulation of axonal outgrowth from adult SCG.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
MEK, Peripheral nerve, Regeneration
in
Journal of Neuroscience Research
volume
52
issue
4
pages
453 - 457
publisher
John Wiley & Sons
external identifiers
  • Scopus:0032524456
ISSN
0360-4012
DOI
10.1002/(SICI)1097-4547(19980515)52:4<453::AID-JNR9>3.0.CO;2-8
language
English
LU publication?
no
id
2b7d1443-d6a3-49b6-a8fb-2c20d84c71d7
date added to LUP
2016-12-07 14:48:47
date last changed
2017-01-20 16:29:54
@article{2b7d1443-d6a3-49b6-a8fb-2c20d84c71d7,
  abstract     = {<p>We have used adult mouse superior cervical ganglia (SCG) to study the role of mitogen activated protein (MAP) kinase activity during axonal outgrowth in vitro. An initial peak in activity within the first hours of culture was followed by a substantially higher activity after 1 to 2 days, a time when axons were actively growing. The latter peak is probably a result of both higher levels of protein and increased activity. The addition of nerve growth factor stimulated both outgrowth and kinase activity, whereas treating the cultures with the kinase inhibitor PD98059 had an opposite effect. Taken together, the results suggest that activation of the MAP kinase pathway could be involved in the initiation as well as regulation of axonal outgrowth from adult SCG.</p>},
  author       = {Svensson, Bodil and Ekström, Per},
  issn         = {0360-4012},
  keyword      = {MEK,Peripheral nerve,Regeneration},
  language     = {eng},
  month        = {05},
  number       = {4},
  pages        = {453--457},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Neuroscience Research},
  title        = {Mitogen activated protein (MAP) kinase activity is increased in adult mouse superior cervical ganglia during culturing},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-4547(19980515)52:4<453::AID-JNR9>3.0.CO;2-8},
  volume       = {52},
  year         = {1998},
}