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Cellobiose dehydrogenase modified electrodes: advances by materials science and biochemical engineering

Ludwig, Roland; Ortiz, Roberto LU ; Schulz, Christopher LU ; Harreither, Wolfgang; Sygmund, Christoph and Gorton, Lo LU (2013) In Analytical and Bioanalytical Chemistry 405(11). p.3637-3658
Abstract
The flavocytochrome cellobiose dehydrogenase (CDH) is a versatile biorecognition element capable of detecting carbohydrates as well as quinones and catecholamines. In addition, it can be used as an anode biocatalyst for enzymatic biofuel cells to power miniaturised sensor-transmitter systems. Various electrode materials and designs have been tested in the past decade to utilize and enhance the direct electron transfer (DET) from the enzyme to the electrode. Additionally, mediated electron transfer (MET) approaches via soluble redox mediators and redox polymers have been pursued. Biosensors for cellobiose, lactose and glucose determination are based on CDH from different fungal producers, which show differences with respect to substrate... (More)
The flavocytochrome cellobiose dehydrogenase (CDH) is a versatile biorecognition element capable of detecting carbohydrates as well as quinones and catecholamines. In addition, it can be used as an anode biocatalyst for enzymatic biofuel cells to power miniaturised sensor-transmitter systems. Various electrode materials and designs have been tested in the past decade to utilize and enhance the direct electron transfer (DET) from the enzyme to the electrode. Additionally, mediated electron transfer (MET) approaches via soluble redox mediators and redox polymers have been pursued. Biosensors for cellobiose, lactose and glucose determination are based on CDH from different fungal producers, which show differences with respect to substrate specificity, pH optima, DET efficiency and surface binding affinity. Biosensors for the detection of quinones and catecholamines can use carbohydrates for analyte regeneration and signal amplification. This review discusses different approaches to enhance the sensitivity and selectivity of CDH-based biosensors, which focus on (1) more efficient DET on chemically modified or nanostructured electrodes, (2) the synthesis of custom-made redox polymers for higher MET currents and (3) the engineering of enzymes and reaction pathways. Combination of these strategies will enable the design of sensitive and selective CDH-based biosensors with reduced electrode size for the detection of analytes in continuous on-site and point-of-care applications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biosensors, Carbohydrates, Catecholamines, Cellobiose dehydrogenase, Electron transfer, Nanomaterials
in
Analytical and Bioanalytical Chemistry
volume
405
issue
11
pages
3637 - 3658
publisher
Springer
external identifiers
  • wos:000317643100019
  • scopus:84877130297
ISSN
1618-2642
DOI
10.1007/s00216-012-6627-x
language
English
LU publication?
yes
id
2b980dbf-bac0-4aca-9dae-b986c040ec75 (old id 3854764)
date added to LUP
2013-06-19 15:06:45
date last changed
2019-10-23 01:00:11
@article{2b980dbf-bac0-4aca-9dae-b986c040ec75,
  abstract     = {The flavocytochrome cellobiose dehydrogenase (CDH) is a versatile biorecognition element capable of detecting carbohydrates as well as quinones and catecholamines. In addition, it can be used as an anode biocatalyst for enzymatic biofuel cells to power miniaturised sensor-transmitter systems. Various electrode materials and designs have been tested in the past decade to utilize and enhance the direct electron transfer (DET) from the enzyme to the electrode. Additionally, mediated electron transfer (MET) approaches via soluble redox mediators and redox polymers have been pursued. Biosensors for cellobiose, lactose and glucose determination are based on CDH from different fungal producers, which show differences with respect to substrate specificity, pH optima, DET efficiency and surface binding affinity. Biosensors for the detection of quinones and catecholamines can use carbohydrates for analyte regeneration and signal amplification. This review discusses different approaches to enhance the sensitivity and selectivity of CDH-based biosensors, which focus on (1) more efficient DET on chemically modified or nanostructured electrodes, (2) the synthesis of custom-made redox polymers for higher MET currents and (3) the engineering of enzymes and reaction pathways. Combination of these strategies will enable the design of sensitive and selective CDH-based biosensors with reduced electrode size for the detection of analytes in continuous on-site and point-of-care applications.},
  author       = {Ludwig, Roland and Ortiz, Roberto and Schulz, Christopher and Harreither, Wolfgang and Sygmund, Christoph and Gorton, Lo},
  issn         = {1618-2642},
  keyword      = {Biosensors,Carbohydrates,Catecholamines,Cellobiose dehydrogenase,Electron transfer,Nanomaterials},
  language     = {eng},
  number       = {11},
  pages        = {3637--3658},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Cellobiose dehydrogenase modified electrodes: advances by materials science and biochemical engineering},
  url          = {http://dx.doi.org/10.1007/s00216-012-6627-x},
  volume       = {405},
  year         = {2013},
}