Genomic deletions and precise removal of transposable elements mediated by short identical DNA segments in primates
van de Lagemaat, LN ; Gagnier, L ; Medstrand, Patrik LU
and Mager, DL
(2005)
In Genome Research
15(9).
p.1243-1249
- Abstract
- Insertion of transposable elements is a major cause of genomic expansion in eukaryotes. Less is understood, however, about mechanisms underlying contraction of genomes. In this study, we show that retroelements can, in rare cases, be precisely deleted from primate genomes, most likely via recombination between 10- to 20-bp target site duplications (TSDs) flanking the retroelement. The deleted loci are indistinguishable from pre-integration sites, effectively reversing the insertion. Through human-chimpanzee-Rhesus monkey genomic comparisons, we estimate that 0.5%-1% of apparent retroelement "insertions" distinguishing humans and chimpanzees actually represent deletions. Furthermore, we demonstrate that 19% of genomic deletions of 200-500... (More)
- Insertion of transposable elements is a major cause of genomic expansion in eukaryotes. Less is understood, however, about mechanisms underlying contraction of genomes. In this study, we show that retroelements can, in rare cases, be precisely deleted from primate genomes, most likely via recombination between 10- to 20-bp target site duplications (TSDs) flanking the retroelement. The deleted loci are indistinguishable from pre-integration sites, effectively reversing the insertion. Through human-chimpanzee-Rhesus monkey genomic comparisons, we estimate that 0.5%-1% of apparent retroelement "insertions" distinguishing humans and chimpanzees actually represent deletions. Furthermore, we demonstrate that 19% of genomic deletions of 200-500 bp that have occurred since the human-chimpanzee divergence are associated with flanking identical repeats of at least 10 bp. A large number of deletions internal to Alu elements were also found flanked by homologies. These results suggest that illegitimate recombination between short direct repeats has played a significant role in human genome evolution. Moreover, this study lends perspective to the view that insertions of retroelements represent unidirectional genetic events. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/224962
- author
- van de Lagemaat, LN
; Gagnier, L
; Medstrand, Patrik
LU
and Mager, DL
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Genome Research
- volume
- 15
- issue
- 9
- pages
- 1243 - 1249
- publisher
- Cold Spring Harbor Laboratory Press (CSHL)
- external identifiers
-
- wos:000231720500007
- pmid:16140992
- scopus:24344509193
- ISSN
- 1549-5469
- DOI
- 10.1101/gr.3910705
- language
- English
- LU publication?
- yes
- id
- 2bb21e06-f58f-4891-a47b-c216be69da8c (old id 224962)
- date added to LUP
- 2016-04-01 16:35:33
- date last changed
- 2022-01-28 20:44:57
@article{2bb21e06-f58f-4891-a47b-c216be69da8c,
abstract = {{Insertion of transposable elements is a major cause of genomic expansion in eukaryotes. Less is understood, however, about mechanisms underlying contraction of genomes. In this study, we show that retroelements can, in rare cases, be precisely deleted from primate genomes, most likely via recombination between 10- to 20-bp target site duplications (TSDs) flanking the retroelement. The deleted loci are indistinguishable from pre-integration sites, effectively reversing the insertion. Through human-chimpanzee-Rhesus monkey genomic comparisons, we estimate that 0.5%-1% of apparent retroelement "insertions" distinguishing humans and chimpanzees actually represent deletions. Furthermore, we demonstrate that 19% of genomic deletions of 200-500 bp that have occurred since the human-chimpanzee divergence are associated with flanking identical repeats of at least 10 bp. A large number of deletions internal to Alu elements were also found flanked by homologies. These results suggest that illegitimate recombination between short direct repeats has played a significant role in human genome evolution. Moreover, this study lends perspective to the view that insertions of retroelements represent unidirectional genetic events.}},
author = {{van de Lagemaat, LN and Gagnier, L and Medstrand, Patrik and Mager, DL}},
issn = {{1549-5469}},
language = {{eng}},
number = {{9}},
pages = {{1243--1249}},
publisher = {{Cold Spring Harbor Laboratory Press (CSHL)}},
series = {{Genome Research}},
title = {{Genomic deletions and precise removal of transposable elements mediated by short identical DNA segments in primates}},
url = {{http://dx.doi.org/10.1101/gr.3910705}},
doi = {{10.1101/gr.3910705}},
volume = {{15}},
year = {{2005}},
}