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Characterization of the receptor response for the neuropeptide Y-evoked suppression of parasympathetically-mediated contractions in the guinea pig trachea

Grundemar, L LU (1997) In Regulatory Peptides 71(2). p.97-101
Abstract

Neuropeptide Y (NPY) acts via several distinct receptor types. The aim of the present study was to examine which NPY receptors are coupled to inhibition of parasympathetically-mediated contractions of the isolated guinea pig trachea. Electrical field stimulation of tracheal rings evoked a rapid twitch, which was abolished by atropine (1 microM). NPY, the structurally related hormone peptide YY (PYY), the Y2 receptor agonist [Cys2, Aoc5-24, D-Cys27]NPY, as well as NPY 5-36 and NPY 13-36 evoked a concentration-dependent inhibition of the electrically-stimulated twitches. Pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) failed to prevent the NPY-induced inhibition. Although less potent than NPY, the Y1 (and Y4-Y6)... (More)

Neuropeptide Y (NPY) acts via several distinct receptor types. The aim of the present study was to examine which NPY receptors are coupled to inhibition of parasympathetically-mediated contractions of the isolated guinea pig trachea. Electrical field stimulation of tracheal rings evoked a rapid twitch, which was abolished by atropine (1 microM). NPY, the structurally related hormone peptide YY (PYY), the Y2 receptor agonist [Cys2, Aoc5-24, D-Cys27]NPY, as well as NPY 5-36 and NPY 13-36 evoked a concentration-dependent inhibition of the electrically-stimulated twitches. Pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) failed to prevent the NPY-induced inhibition. Although less potent than NPY, the Y1 (and Y4-Y6) receptor agonist [Leu31, Pro34]NPY also inhibited the electrically-stimulated twitches. Another NPY-related peptide, pancreatic polypeptide, which recognizes Y4-Y6 receptors did not affect the stimulated twitches at concentrations up to 1 microM. However, pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) virtually abolished the inhibition evoked by [Leu31, Pro34]NPY. None of the peptides affected the baseline tension and BIBP3226 (1 microM) per se did not affect the amplitude of the electrically-stimulated twitches. In conclusion, it seems that NPY and PYY are capable of suppressing parasympathetically mediated contractions in the guinea pig trachea mainly via Y2 receptors, but there is also a small contribution from Y1 receptors.

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author
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Contribution to journal
publication status
published
subject
keywords
Animals, Arginine/analogs & derivatives, Atropine/pharmacology, Dose-Response Relationship, Drug, Electric Stimulation, Guinea Pigs, In Vitro Techniques, Muscle Contraction/drug effects, Muscle, Smooth/drug effects, Neuropeptide Y/analogs & derivatives, Pancreatic Polypeptide/pharmacology, Peptide Fragments/pharmacology, Peptide YY/pharmacology, Receptors, Neuropeptide Y/antagonists & inhibitors, Trachea/innervation
in
Regulatory Peptides
volume
71
issue
2
pages
97 - 101
publisher
Elsevier
external identifiers
  • pmid:9416991
ISSN
0167-0115
DOI
10.1016/s0167-0115(97)01023-9
language
English
LU publication?
no
id
2bd232ec-8c72-426f-9c6b-eaacaba56cea
date added to LUP
2019-09-03 13:55:51
date last changed
2019-12-04 04:02:27
@article{2bd232ec-8c72-426f-9c6b-eaacaba56cea,
  abstract     = {<p>Neuropeptide Y (NPY) acts via several distinct receptor types. The aim of the present study was to examine which NPY receptors are coupled to inhibition of parasympathetically-mediated contractions of the isolated guinea pig trachea. Electrical field stimulation of tracheal rings evoked a rapid twitch, which was abolished by atropine (1 microM). NPY, the structurally related hormone peptide YY (PYY), the Y2 receptor agonist [Cys2, Aoc5-24, D-Cys27]NPY, as well as NPY 5-36 and NPY 13-36 evoked a concentration-dependent inhibition of the electrically-stimulated twitches. Pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) failed to prevent the NPY-induced inhibition. Although less potent than NPY, the Y1 (and Y4-Y6) receptor agonist [Leu31, Pro34]NPY also inhibited the electrically-stimulated twitches. Another NPY-related peptide, pancreatic polypeptide, which recognizes Y4-Y6 receptors did not affect the stimulated twitches at concentrations up to 1 microM. However, pretreatment with the Y1 receptor-selective antagonist BIBP3226 (1 microM) virtually abolished the inhibition evoked by [Leu31, Pro34]NPY. None of the peptides affected the baseline tension and BIBP3226 (1 microM) per se did not affect the amplitude of the electrically-stimulated twitches. In conclusion, it seems that NPY and PYY are capable of suppressing parasympathetically mediated contractions in the guinea pig trachea mainly via Y2 receptors, but there is also a small contribution from Y1 receptors.</p>},
  author       = {Grundemar, L},
  issn         = {0167-0115},
  language     = {eng},
  month        = {08},
  number       = {2},
  pages        = {97--101},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Characterization of the receptor response for the neuropeptide Y-evoked suppression of parasympathetically-mediated contractions in the guinea pig trachea},
  url          = {http://dx.doi.org/10.1016/s0167-0115(97)01023-9},
  doi          = {10.1016/s0167-0115(97)01023-9},
  volume       = {71},
  year         = {1997},
}