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Adenosine inhibition of the regeneration in vitro of adult frog sciatic sensory axons

Edström, Anders LU ; Edbladh, Magnus LU and Ekström, Per LU (1992) In Brain Research 570(1-2). p.35-41
Abstract

The sensory axons of the adult frog sciatic nerve have earlier been shown to regenerate in vitro. If a local test crush is made at the initiation of culturing, regeneration starts after 3.4 days and proceeds at a rate of about 0.8-0.9 mm/day for several days. In the present experiments regeneration was inhibited by adenosine in a reversible and dose-dependent fashion. Similarly, both an adenosine analogue, 2-chloroadenosine (2-CA), and a non-hydrolyzable ATP analogue, AMP-PNP, reduced the outgrowth of sensory axons. The effect of adenosine was partially antagonized by theophylline at a critical concentration. Using a compartmental system, it could clearly be shown that adenosine exerted its effects at the outgrowth region. Adenosine,... (More)

The sensory axons of the adult frog sciatic nerve have earlier been shown to regenerate in vitro. If a local test crush is made at the initiation of culturing, regeneration starts after 3.4 days and proceeds at a rate of about 0.8-0.9 mm/day for several days. In the present experiments regeneration was inhibited by adenosine in a reversible and dose-dependent fashion. Similarly, both an adenosine analogue, 2-chloroadenosine (2-CA), and a non-hydrolyzable ATP analogue, AMP-PNP, reduced the outgrowth of sensory axons. The effect of adenosine was partially antagonized by theophylline at a critical concentration. Using a compartmental system, it could clearly be shown that adenosine exerted its effects at the outgrowth region. Adenosine, 2-CA, and AMP-PNP were also found to inhibit the proliferation of Schwann cells in the regenerating nerve. Various experiments showed that the latter can not explain the outgrowth inhibitory effects, which could be mediated by adenosine receptors associated with the elongating axons.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adenosine, Adenosine receptor, Cyclic AMP, Frog sciatic nerve, In vitro, Nerve regeneration, Schwann cell
in
Brain Research
volume
570
issue
1-2
pages
35 - 41
publisher
Elsevier
external identifiers
  • pmid:1617426
  • scopus:0026542875
ISSN
0006-8993
DOI
10.1016/0006-8993(92)90560-V
language
English
LU publication?
yes
id
2beeb2e0-3f78-4a29-8c5c-a93946f06417
date added to LUP
2016-12-07 14:38:19
date last changed
2025-01-12 17:07:11
@article{2beeb2e0-3f78-4a29-8c5c-a93946f06417,
  abstract     = {{<p>The sensory axons of the adult frog sciatic nerve have earlier been shown to regenerate in vitro. If a local test crush is made at the initiation of culturing, regeneration starts after 3.4 days and proceeds at a rate of about 0.8-0.9 mm/day for several days. In the present experiments regeneration was inhibited by adenosine in a reversible and dose-dependent fashion. Similarly, both an adenosine analogue, 2-chloroadenosine (2-CA), and a non-hydrolyzable ATP analogue, AMP-PNP, reduced the outgrowth of sensory axons. The effect of adenosine was partially antagonized by theophylline at a critical concentration. Using a compartmental system, it could clearly be shown that adenosine exerted its effects at the outgrowth region. Adenosine, 2-CA, and AMP-PNP were also found to inhibit the proliferation of Schwann cells in the regenerating nerve. Various experiments showed that the latter can not explain the outgrowth inhibitory effects, which could be mediated by adenosine receptors associated with the elongating axons.</p>}},
  author       = {{Edström, Anders and Edbladh, Magnus and Ekström, Per}},
  issn         = {{0006-8993}},
  keywords     = {{Adenosine; Adenosine receptor; Cyclic AMP; Frog sciatic nerve; In vitro; Nerve regeneration; Schwann cell}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1-2}},
  pages        = {{35--41}},
  publisher    = {{Elsevier}},
  series       = {{Brain Research}},
  title        = {{Adenosine inhibition of the regeneration in vitro of adult frog sciatic sensory axons}},
  url          = {{http://dx.doi.org/10.1016/0006-8993(92)90560-V}},
  doi          = {{10.1016/0006-8993(92)90560-V}},
  volume       = {{570}},
  year         = {{1992}},
}