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Molecularly imprinted supermacroporous cryogels for myoglobin recognition

Ertürk, Gizem LU ; Bereli, Nilay; Ramteke, Pramod W and Denizli, Adil (2014) In Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology 173(5). p.62-1250
Abstract

Myoglobin is a primary iron, and oxygen-binding protein of muscle tissues and levels can be an important diagnostic biomarker for acute myocardial infarction, myocardial necrosis, or other cardiac diseases. The establishment of myoglobin recognition systems is important because of its protein's structural and functional values in physiology, biochemistry, and diagnostic value in some damaged muscle tissue and cardiac diseases. For this purpose, we used molecular imprinting technique for myoglobin recognition from aqueous solutions and human plasma. In the first step, myoglobin-imprinted poly(hydroxyethyl methacrylate) (PHEMA) cryogels (MGb-MIP) were prepared, and optimum myoglobin adsorption conditions were determined. Selectivity... (More)

Myoglobin is a primary iron, and oxygen-binding protein of muscle tissues and levels can be an important diagnostic biomarker for acute myocardial infarction, myocardial necrosis, or other cardiac diseases. The establishment of myoglobin recognition systems is important because of its protein's structural and functional values in physiology, biochemistry, and diagnostic value in some damaged muscle tissue and cardiac diseases. For this purpose, we used molecular imprinting technique for myoglobin recognition from aqueous solutions and human plasma. In the first step, myoglobin-imprinted poly(hydroxyethyl methacrylate) (PHEMA) cryogels (MGb-MIP) were prepared, and optimum myoglobin adsorption conditions were determined. Selectivity experiments have been done with the competitive proteins, and myoglobin adsorption from IgG and albumin-free human plasma was studied. The purity of the desorbed samples was determined with SDS-PAGE. The desorption efficiency and reusability of the MGb-MIP cryogels were tested, and it was shown that without any significant loss in the adsorption capacity, MGb-MIP cryogels can be used a number of times for myoglobin recognition and separation.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Adsorption, Animals, Cryogels, Diffusion, Humans, Hydrogen-Ion Concentration, Molecular Imprinting, Myoglobin, Polyhydroxyethyl Methacrylate, Porosity, Temperature, Water, Journal Article
in
Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
volume
173
issue
5
pages
62 - 1250
publisher
Humana Press
external identifiers
  • scopus:84904857003
ISSN
1559-0291
DOI
10.1007/s12010-014-0844-z
language
English
LU publication?
no
id
2c53b838-adf7-4e88-a1a1-1d7edd43fce1
date added to LUP
2018-01-25 13:49:14
date last changed
2019-02-20 11:05:51
@article{2c53b838-adf7-4e88-a1a1-1d7edd43fce1,
  abstract     = {<p>Myoglobin is a primary iron, and oxygen-binding protein of muscle tissues and levels can be an important diagnostic biomarker for acute myocardial infarction, myocardial necrosis, or other cardiac diseases. The establishment of myoglobin recognition systems is important because of its protein's structural and functional values in physiology, biochemistry, and diagnostic value in some damaged muscle tissue and cardiac diseases. For this purpose, we used molecular imprinting technique for myoglobin recognition from aqueous solutions and human plasma. In the first step, myoglobin-imprinted poly(hydroxyethyl methacrylate) (PHEMA) cryogels (MGb-MIP) were prepared, and optimum myoglobin adsorption conditions were determined. Selectivity experiments have been done with the competitive proteins, and myoglobin adsorption from IgG and albumin-free human plasma was studied. The purity of the desorbed samples was determined with SDS-PAGE. The desorption efficiency and reusability of the MGb-MIP cryogels were tested, and it was shown that without any significant loss in the adsorption capacity, MGb-MIP cryogels can be used a number of times for myoglobin recognition and separation.</p>},
  author       = {Ertürk, Gizem and Bereli, Nilay and Ramteke, Pramod W and Denizli, Adil},
  issn         = {1559-0291},
  keyword      = {Adsorption,Animals,Cryogels,Diffusion,Humans,Hydrogen-Ion Concentration,Molecular Imprinting,Myoglobin,Polyhydroxyethyl Methacrylate,Porosity,Temperature,Water,Journal Article},
  language     = {eng},
  number       = {5},
  pages        = {62--1250},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology},
  title        = {Molecularly imprinted supermacroporous cryogels for myoglobin recognition},
  url          = {http://dx.doi.org/10.1007/s12010-014-0844-z},
  volume       = {173},
  year         = {2014},
}