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Enzyme-Functionalized Cellulose Beads as a Promising Antimicrobial Material

Califano, Davide ; Patenall, Bethany Lee ; Kadowaki, Marco A.S. ; Mattia, Davide ; Scott, Janet L. and Edler, Karen J. LU orcid (2021) In Biomacromolecules 22(2). p.754-762
Abstract
The extensive use of antibiotics over the last decades is responsible
for the emergence of multidrug-resistant (MDR) microorganisms that are
challenging health care systems worldwide. The use of alternative
antimicrobial materials could mitigate the selection of new MDR strains
by reducing antibiotic overuse. This paper describes the design of
enzyme-based antimicrobial cellulose beads containing a covalently
coupled glucose oxidase from Aspergillus niger (GOx) able to release antimicrobial concentrations of hydrogen peroxide (H2O2)
(≈ 1.8 mM). The material preparation was optimized to obtain the best
performance in terms of mechanical resistance, shelf life, and... (More)
The extensive use of antibiotics over the last decades is responsible
for the emergence of multidrug-resistant (MDR) microorganisms that are
challenging health care systems worldwide. The use of alternative
antimicrobial materials could mitigate the selection of new MDR strains
by reducing antibiotic overuse. This paper describes the design of
enzyme-based antimicrobial cellulose beads containing a covalently
coupled glucose oxidase from Aspergillus niger (GOx) able to release antimicrobial concentrations of hydrogen peroxide (H2O2)
(≈ 1.8 mM). The material preparation was optimized to obtain the best
performance in terms of mechanical resistance, shelf life, and H2O2
production. As a proof of concept, agar inhibition halo assays
(Kirby-Bauer test) against model pathogens were performed. The two most
relevant factors affecting the bead functionalization process were the
degree of oxidation and the pH used for the enzyme binding process.
Slightly acidic conditions during the functionalization process (pH 6)
showed the best results for the GOx/cellulose system. The functionalized
beads inhibited the growth of all the microorganisms assayed,
confirming the release of sufficient antimicrobial levels of H2O2. The maximum inhibition efficiency was exhibited toward Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli), although significant inhibitory effects toward methicillin-resistant Staphylococcus aureus (MRSA) and S. aureus
were also observed. These enzyme-functionalized cellulose beads
represent an inexpensive, sustainable, and biocompatible antimicrobial
material with potential use in many applications, including the
manufacturing of biomedical products and additives for food
preservation. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Biomacromolecules
volume
22
issue
2
pages
9 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:33404227
  • scopus:85099658015
ISSN
1525-7797
DOI
10.1021/acs.biomac.0c01536
language
English
LU publication?
no
additional info
Publisher Copyright: © 2020 American Chemical Society.
id
2c5fdc5e-26c4-40b5-a02b-ce6c36a6de14
date added to LUP
2023-01-18 08:59:20
date last changed
2024-04-18 10:58:05
@article{2c5fdc5e-26c4-40b5-a02b-ce6c36a6de14,
  abstract     = {{The extensive use of antibiotics over the last decades is responsible <br>
for the emergence of multidrug-resistant (MDR) microorganisms that are <br>
challenging health care systems worldwide. The use of alternative <br>
antimicrobial materials could mitigate the selection of new MDR strains <br>
by reducing antibiotic overuse. This paper describes the design of <br>
enzyme-based antimicrobial cellulose beads containing a covalently <br>
coupled glucose oxidase from <i>Aspergillus niger</i> (GOx) able to release antimicrobial concentrations of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)<br>
 (≈ 1.8 mM). The material preparation was optimized to obtain the best <br>
performance in terms of mechanical resistance, shelf life, and H<sub>2</sub>O<sub>2</sub><br>
 production. As a proof of concept, agar inhibition halo assays <br>
(Kirby-Bauer test) against model pathogens were performed. The two most <br>
relevant factors affecting the bead functionalization process were the <br>
degree of oxidation and the pH used for the enzyme binding process. <br>
Slightly acidic conditions during the functionalization process (pH 6) <br>
showed the best results for the GOx/cellulose system. The functionalized<br>
 beads inhibited the growth of all the microorganisms assayed, <br>
confirming the release of sufficient antimicrobial levels of H<sub>2</sub>O<sub>2</sub>. The maximum inhibition efficiency was exhibited toward <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) and <i>Escherichia coli</i> (<i>E. coli</i>), although significant inhibitory effects toward methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and <i>S. aureus</i><br>
 were also observed. These enzyme-functionalized cellulose beads <br>
represent an inexpensive, sustainable, and biocompatible antimicrobial <br>
material with potential use in many applications, including the <br>
manufacturing of biomedical products and additives for food <br>
preservation.}},
  author       = {{Califano, Davide and Patenall, Bethany Lee and Kadowaki, Marco A.S. and Mattia, Davide and Scott, Janet L. and Edler, Karen J.}},
  issn         = {{1525-7797}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{2}},
  pages        = {{754--762}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Biomacromolecules}},
  title        = {{Enzyme-Functionalized Cellulose Beads as a Promising Antimicrobial Material}},
  url          = {{http://dx.doi.org/10.1021/acs.biomac.0c01536}},
  doi          = {{10.1021/acs.biomac.0c01536}},
  volume       = {{22}},
  year         = {{2021}},
}