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Time-resolved fluorescence imaging for quantitative histochemistry using lanthanide chelates in nanoparticles and conjugated to monoclonal antibodies

Väisänen, Ville ; Härmä, Harri ; Lilja, Hans LU orcid and Bjartell, Anders LU (2000) In Luminescence 15(6). p.389-397
Abstract

Tissue and cell examinations have a potential to produce extremely valuable information about antigen quantities in samples. Using currently available methods, a truly quantitative analysis is nearly impossible. We have previously shown that immunohistochemical (IHC) detection of prostate-specific antigen and human glandular kallikrein from prostatic tissue, together with time-resolved fluorescence imaging (TRFI), is a suitable method for obtaining quantitative data from biological samples and that the signal response is linear. In this paper we show that Eu-chelate containing particles in the nanometer range are suitable labels for quantitative IHC. Even single nanoparticle molecules can be detected by TRFI and the signals measured can... (More)

Tissue and cell examinations have a potential to produce extremely valuable information about antigen quantities in samples. Using currently available methods, a truly quantitative analysis is nearly impossible. We have previously shown that immunohistochemical (IHC) detection of prostate-specific antigen and human glandular kallikrein from prostatic tissue, together with time-resolved fluorescence imaging (TRFI), is a suitable method for obtaining quantitative data from biological samples and that the signal response is linear. In this paper we show that Eu-chelate containing particles in the nanometer range are suitable labels for quantitative IHC. Even single nanoparticle molecules can be detected by TRFI and the signals measured can be readily quantitated. The signal intensity correlates very well with the amount of bound label, and the use of nanoparticles could markedly improve the sensitivity of quantitative IHC methods. TRFI provides a powerful tool for providing quantitative data about antigens or transcripts in tissue sections or cultured cells. It is also of major importance in standardization and optimization of protocols for fixation and tissue preparation, including antigen retrieval methods.

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Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Image analysis, Immunohistochemistry, Lanthanide chelates, Nanoparticles
in
Luminescence
volume
15
issue
6
pages
389 - 397
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:11114116
  • scopus:0034330678
ISSN
1522-7235
DOI
10.1002/1522-7243(200011/12)15:6<389::aid-bio626>3.0.co;2-7
language
English
LU publication?
yes
id
2c6b6480-846c-44d8-bc24-03d74e70c3fb
date added to LUP
2022-12-06 17:31:06
date last changed
2024-06-23 10:19:17
@article{2c6b6480-846c-44d8-bc24-03d74e70c3fb,
  abstract     = {{<p>Tissue and cell examinations have a potential to produce extremely valuable information about antigen quantities in samples. Using currently available methods, a truly quantitative analysis is nearly impossible. We have previously shown that immunohistochemical (IHC) detection of prostate-specific antigen and human glandular kallikrein from prostatic tissue, together with time-resolved fluorescence imaging (TRFI), is a suitable method for obtaining quantitative data from biological samples and that the signal response is linear. In this paper we show that Eu-chelate containing particles in the nanometer range are suitable labels for quantitative IHC. Even single nanoparticle molecules can be detected by TRFI and the signals measured can be readily quantitated. The signal intensity correlates very well with the amount of bound label, and the use of nanoparticles could markedly improve the sensitivity of quantitative IHC methods. TRFI provides a powerful tool for providing quantitative data about antigens or transcripts in tissue sections or cultured cells. It is also of major importance in standardization and optimization of protocols for fixation and tissue preparation, including antigen retrieval methods.</p>}},
  author       = {{Väisänen, Ville and Härmä, Harri and Lilja, Hans and Bjartell, Anders}},
  issn         = {{1522-7235}},
  keywords     = {{Image analysis; Immunohistochemistry; Lanthanide chelates; Nanoparticles}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{389--397}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Luminescence}},
  title        = {{Time-resolved fluorescence imaging for quantitative histochemistry using lanthanide chelates in nanoparticles and conjugated to monoclonal antibodies}},
  url          = {{http://dx.doi.org/10.1002/1522-7243(200011/12)15:6<389::aid-bio626>3.0.co;2-7}},
  doi          = {{10.1002/1522-7243(200011/12)15:6<389::aid-bio626>3.0.co;2-7}},
  volume       = {{15}},
  year         = {{2000}},
}