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Cortex synthesis during Bacillus subtilis sporulation depends on the transpeptidase activity of SpoVD.

Bukowska-Faniband, Ewa LU and Hederstedt, Lars LU (2013) In FEMS Microbiology Letters 346(1). p.65-72
Abstract
The nonessential process of peptidoglycan synthesis during Bacillus subtilis sporulation is one model to study bacterial cell wall biogenesis. SpoVD is a class B high-molecular weight penicillin-binding protein that is specific for sporulation. Strains lacking this protein produce spores without the peptidoglycan cortex layer and are heat-sensitive. The detailed functions of the four different protein domains of the SpoVD protein are unknown and the observed phenotype of strains lacking the entire protein could be an indirect defect. We therefore inactivated the transpeptidase domain by substitution of the active site serine residue. Our results demonstrate that endospore cortex synthesis depends on the transpeptidase activity of SpoVD... (More)
The nonessential process of peptidoglycan synthesis during Bacillus subtilis sporulation is one model to study bacterial cell wall biogenesis. SpoVD is a class B high-molecular weight penicillin-binding protein that is specific for sporulation. Strains lacking this protein produce spores without the peptidoglycan cortex layer and are heat-sensitive. The detailed functions of the four different protein domains of the SpoVD protein are unknown and the observed phenotype of strains lacking the entire protein could be an indirect defect. We therefore inactivated the transpeptidase domain by substitution of the active site serine residue. Our results demonstrate that endospore cortex synthesis depends on the transpeptidase activity of SpoVD specifically. This article is protected by copyright. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
penicillin-binding protein, peptidoglycan, endospore.
in
FEMS Microbiology Letters
volume
346
issue
1
pages
65 - 72
publisher
Oxford University Press
external identifiers
  • wos:000322715600008
  • pmid:23789716
  • scopus:84881372843
ISSN
1574-6968
DOI
10.1111/1574-6968.12202
language
English
LU publication?
yes
id
2c977765-7741-4436-9710-90eaab8425d4 (old id 3913086)
date added to LUP
2016-04-01 10:00:18
date last changed
2022-03-27 03:54:49
@article{2c977765-7741-4436-9710-90eaab8425d4,
  abstract     = {{The nonessential process of peptidoglycan synthesis during Bacillus subtilis sporulation is one model to study bacterial cell wall biogenesis. SpoVD is a class B high-molecular weight penicillin-binding protein that is specific for sporulation. Strains lacking this protein produce spores without the peptidoglycan cortex layer and are heat-sensitive. The detailed functions of the four different protein domains of the SpoVD protein are unknown and the observed phenotype of strains lacking the entire protein could be an indirect defect. We therefore inactivated the transpeptidase domain by substitution of the active site serine residue. Our results demonstrate that endospore cortex synthesis depends on the transpeptidase activity of SpoVD specifically. This article is protected by copyright. All rights reserved.}},
  author       = {{Bukowska-Faniband, Ewa and Hederstedt, Lars}},
  issn         = {{1574-6968}},
  keywords     = {{penicillin-binding protein; peptidoglycan; endospore.}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{65--72}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Microbiology Letters}},
  title        = {{Cortex synthesis during <em>Bacillus subtilis</em> sporulation depends on the transpeptidase activity of SpoVD.}},
  url          = {{http://dx.doi.org/10.1111/1574-6968.12202}},
  doi          = {{10.1111/1574-6968.12202}},
  volume       = {{346}},
  year         = {{2013}},
}