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ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency

Pereira, Carlos F. LU orcid ; Piccolo, Francesco M. ; Tsubouchi, Tomomi ; Sauer, Stephan P A ; Ryan, Natalie K. ; Bruno, Ludovica ; Landeira, David ; Santos, Joana ; Banito, Ana and Gil, Jesus , et al. (2010) In Cell Stem Cell 6(6). p.547-556
Abstract

Embryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG proteins are required for establishing, rather than maintaining, the pluripotent state, we compared the ability of wild-type, PRC1-, and PRC2-depleted ESCs to reprogram human lymphocytes. We show that ESCs lacking either PRC1 or PRC2 are unable to successfully reprogram B cells toward pluripotency. This defect is a direct consequence of the lack of PcG activity because it could be efficiently rescued by reconstituting PRC2... (More)

Embryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG proteins are required for establishing, rather than maintaining, the pluripotent state, we compared the ability of wild-type, PRC1-, and PRC2-depleted ESCs to reprogram human lymphocytes. We show that ESCs lacking either PRC1 or PRC2 are unable to successfully reprogram B cells toward pluripotency. This defect is a direct consequence of the lack of PcG activity because it could be efficiently rescued by reconstituting PRC2 activity in PRC2-deficient ESCs. Surprisingly, the failure of PRC2-deficient ESCs to reprogram somatic cells is functionally dominant, demonstrating a critical requirement for PcG proteins in the chromatin-remodeling events required for the direct conversion of differentiated cells toward pluripotency.

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publishing date
type
Contribution to journal
publication status
published
in
Cell Stem Cell
volume
6
issue
6
pages
10 pages
publisher
Cell Press
external identifiers
  • pmid:20569692
  • scopus:77953086963
ISSN
1934-5909
DOI
10.1016/j.stem.2010.04.013
language
English
LU publication?
no
id
2ccf8114-2505-4801-921a-ff5d55b388ad
date added to LUP
2017-10-02 17:30:51
date last changed
2024-02-29 22:53:28
@article{2ccf8114-2505-4801-921a-ff5d55b388ad,
  abstract     = {{<p>Embryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG proteins are required for establishing, rather than maintaining, the pluripotent state, we compared the ability of wild-type, PRC1-, and PRC2-depleted ESCs to reprogram human lymphocytes. We show that ESCs lacking either PRC1 or PRC2 are unable to successfully reprogram B cells toward pluripotency. This defect is a direct consequence of the lack of PcG activity because it could be efficiently rescued by reconstituting PRC2 activity in PRC2-deficient ESCs. Surprisingly, the failure of PRC2-deficient ESCs to reprogram somatic cells is functionally dominant, demonstrating a critical requirement for PcG proteins in the chromatin-remodeling events required for the direct conversion of differentiated cells toward pluripotency.</p>}},
  author       = {{Pereira, Carlos F. and Piccolo, Francesco M. and Tsubouchi, Tomomi and Sauer, Stephan P A and Ryan, Natalie K. and Bruno, Ludovica and Landeira, David and Santos, Joana and Banito, Ana and Gil, Jesus and Koseki, Haruhiko and Merkenschlager, Matthias and Fisher, Amanda G.}},
  issn         = {{1934-5909}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{6}},
  pages        = {{547--556}},
  publisher    = {{Cell Press}},
  series       = {{Cell Stem Cell}},
  title        = {{ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency}},
  url          = {{http://dx.doi.org/10.1016/j.stem.2010.04.013}},
  doi          = {{10.1016/j.stem.2010.04.013}},
  volume       = {{6}},
  year         = {{2010}},
}