ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency
(2010) In Cell Stem Cell 6(6). p.547-556- Abstract
Embryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG proteins are required for establishing, rather than maintaining, the pluripotent state, we compared the ability of wild-type, PRC1-, and PRC2-depleted ESCs to reprogram human lymphocytes. We show that ESCs lacking either PRC1 or PRC2 are unable to successfully reprogram B cells toward pluripotency. This defect is a direct consequence of the lack of PcG activity because it could be efficiently rescued by reconstituting PRC2... (More)
Embryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG proteins are required for establishing, rather than maintaining, the pluripotent state, we compared the ability of wild-type, PRC1-, and PRC2-depleted ESCs to reprogram human lymphocytes. We show that ESCs lacking either PRC1 or PRC2 are unable to successfully reprogram B cells toward pluripotency. This defect is a direct consequence of the lack of PcG activity because it could be efficiently rescued by reconstituting PRC2 activity in PRC2-deficient ESCs. Surprisingly, the failure of PRC2-deficient ESCs to reprogram somatic cells is functionally dominant, demonstrating a critical requirement for PcG proteins in the chromatin-remodeling events required for the direct conversion of differentiated cells toward pluripotency.
(Less)
- author
- publishing date
- 2010-06-04
- type
- Contribution to journal
- publication status
- published
- in
- Cell Stem Cell
- volume
- 6
- issue
- 6
- pages
- 10 pages
- publisher
- Cell Press
- external identifiers
-
- pmid:20569692
- scopus:77953086963
- ISSN
- 1934-5909
- DOI
- 10.1016/j.stem.2010.04.013
- language
- English
- LU publication?
- no
- id
- 2ccf8114-2505-4801-921a-ff5d55b388ad
- date added to LUP
- 2017-10-02 17:30:51
- date last changed
- 2024-02-29 22:53:28
@article{2ccf8114-2505-4801-921a-ff5d55b388ad, abstract = {{<p>Embryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG proteins are required for establishing, rather than maintaining, the pluripotent state, we compared the ability of wild-type, PRC1-, and PRC2-depleted ESCs to reprogram human lymphocytes. We show that ESCs lacking either PRC1 or PRC2 are unable to successfully reprogram B cells toward pluripotency. This defect is a direct consequence of the lack of PcG activity because it could be efficiently rescued by reconstituting PRC2 activity in PRC2-deficient ESCs. Surprisingly, the failure of PRC2-deficient ESCs to reprogram somatic cells is functionally dominant, demonstrating a critical requirement for PcG proteins in the chromatin-remodeling events required for the direct conversion of differentiated cells toward pluripotency.</p>}}, author = {{Pereira, Carlos F. and Piccolo, Francesco M. and Tsubouchi, Tomomi and Sauer, Stephan P A and Ryan, Natalie K. and Bruno, Ludovica and Landeira, David and Santos, Joana and Banito, Ana and Gil, Jesus and Koseki, Haruhiko and Merkenschlager, Matthias and Fisher, Amanda G.}}, issn = {{1934-5909}}, language = {{eng}}, month = {{06}}, number = {{6}}, pages = {{547--556}}, publisher = {{Cell Press}}, series = {{Cell Stem Cell}}, title = {{ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency}}, url = {{http://dx.doi.org/10.1016/j.stem.2010.04.013}}, doi = {{10.1016/j.stem.2010.04.013}}, volume = {{6}}, year = {{2010}}, }