Proteomic Changes during B Cell Maturation: 2D-DIGE Approach
Salonen, Johanna ; Ronnholm, Gunilla ; Kalkkinen, Nisse and Vihinen, Mauno LU
(2013)
In PLoS ONE
8(10).
- Abstract
- B cells play a pivotal role in adaptive immune system, since they maintain a delicate balance between recognition and clearance of foreign pathogens and tolerance to self. During maturation, B cells progress through a series of developmental stages defined by specific phenotypic surface markers and the rearrangement and expression of immunoglobulin (Ig) genes. To get insight into B cell proteome during the maturation pathway, we studied differential protein expression in eight human cell lines, which cover four distinctive developmental stages; early pre-B, pre-B, plasma cell and immature B cell upon anti-IgM stimulation. Our two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry based proteomic study indicates... (More)
- B cells play a pivotal role in adaptive immune system, since they maintain a delicate balance between recognition and clearance of foreign pathogens and tolerance to self. During maturation, B cells progress through a series of developmental stages defined by specific phenotypic surface markers and the rearrangement and expression of immunoglobulin (Ig) genes. To get insight into B cell proteome during the maturation pathway, we studied differential protein expression in eight human cell lines, which cover four distinctive developmental stages; early pre-B, pre-B, plasma cell and immature B cell upon anti-IgM stimulation. Our two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry based proteomic study indicates the involvement of large number of proteins with various functions. Notably, proteins related to cytoskeleton were relatively highly expressed in early pre-B and pre-B cells, whereas plasma cell proteome contained endoplasmic reticulum and Golgi system proteins. Our long time series analysis in anti-IgM stimulated Ramos B cells revealed the dynamic regulation of cytoskeleton organization, gene expression and metabolic pathways, among others. The findings are related to cellular processes in B cells and are discussed in relation to experimental information for the proteins and pathways they are involved in. Representative 2D-DIGE maps of different B cell maturation stages are available online at http://structure.bmc.lu.se/BcellProteome/. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4205388
- author
- Salonen, Johanna
; Ronnholm, Gunilla
; Kalkkinen, Nisse
and Vihinen, Mauno
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 8
- issue
- 10
- article number
- e77894
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- wos:000326270700059
- scopus:84906921537
- pmid:24205016
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0077894
- language
- English
- LU publication?
- yes
- id
- 2d00bd2e-1987-4075-82a8-4202299fbfeb (old id 4205388)
- date added to LUP
- 2016-04-01 14:18:27
- date last changed
- 2022-01-27 23:56:29
@article{2d00bd2e-1987-4075-82a8-4202299fbfeb,
abstract = {{B cells play a pivotal role in adaptive immune system, since they maintain a delicate balance between recognition and clearance of foreign pathogens and tolerance to self. During maturation, B cells progress through a series of developmental stages defined by specific phenotypic surface markers and the rearrangement and expression of immunoglobulin (Ig) genes. To get insight into B cell proteome during the maturation pathway, we studied differential protein expression in eight human cell lines, which cover four distinctive developmental stages; early pre-B, pre-B, plasma cell and immature B cell upon anti-IgM stimulation. Our two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry based proteomic study indicates the involvement of large number of proteins with various functions. Notably, proteins related to cytoskeleton were relatively highly expressed in early pre-B and pre-B cells, whereas plasma cell proteome contained endoplasmic reticulum and Golgi system proteins. Our long time series analysis in anti-IgM stimulated Ramos B cells revealed the dynamic regulation of cytoskeleton organization, gene expression and metabolic pathways, among others. The findings are related to cellular processes in B cells and are discussed in relation to experimental information for the proteins and pathways they are involved in. Representative 2D-DIGE maps of different B cell maturation stages are available online at http://structure.bmc.lu.se/BcellProteome/.}},
author = {{Salonen, Johanna and Ronnholm, Gunilla and Kalkkinen, Nisse and Vihinen, Mauno}},
issn = {{1932-6203}},
language = {{eng}},
number = {{10}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{Proteomic Changes during B Cell Maturation: 2D-DIGE Approach}},
url = {{https://lup.lub.lu.se/search/files/3899150/4437971.pdf}},
doi = {{10.1371/journal.pone.0077894}},
volume = {{8}},
year = {{2013}},
}