Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis
(2020) In Journal of Cellular and Molecular Medicine 24(2). p.1980-1992- Abstract
WW domain binding protein 1-like (WBP1L), also known as outcome predictor of acute leukaemia 1 (OPAL1), is a transmembrane adaptor protein, expression of which correlates with ETV6-RUNX1 (t(12;21)(p13;q22)) translocation and favourable prognosis in childhood leukaemia. It has a broad expression pattern in haematopoietic and in non-haematopoietic cells. However, its physiological function has been unknown. Here, we show that WBP1L negatively regulates signalling through a critical chemokine receptor CXCR4 in multiple leucocyte subsets and cell lines. We also show that WBP1L interacts with NEDD4-family ubiquitin ligases and regulates CXCR4 ubiquitination and expression. Moreover, analysis of Wbp1l-deficient mice revealed alterations in B... (More)
WW domain binding protein 1-like (WBP1L), also known as outcome predictor of acute leukaemia 1 (OPAL1), is a transmembrane adaptor protein, expression of which correlates with ETV6-RUNX1 (t(12;21)(p13;q22)) translocation and favourable prognosis in childhood leukaemia. It has a broad expression pattern in haematopoietic and in non-haematopoietic cells. However, its physiological function has been unknown. Here, we show that WBP1L negatively regulates signalling through a critical chemokine receptor CXCR4 in multiple leucocyte subsets and cell lines. We also show that WBP1L interacts with NEDD4-family ubiquitin ligases and regulates CXCR4 ubiquitination and expression. Moreover, analysis of Wbp1l-deficient mice revealed alterations in B cell development and enhanced efficiency of bone marrow cell transplantation. Collectively, our data show that WBP1L is a novel regulator of CXCR4 signalling and haematopoiesis.
(Less)
- author
- publishing date
- 2020-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adaptor Proteins, Signal Transducing/metabolism, Animals, Germ Cells/metabolism, Glycoproteins/metabolism, HEK293 Cells, Hematopoiesis, Hematopoietic Stem Cells/metabolism, Homeostasis, Humans, Lipoylation, Membrane Proteins/genetics, Mice, Inbred C57BL, Protein Binding, RNA, Small Interfering/metabolism, Receptors, CXCR4/metabolism, Signal Transduction, Ubiquitin-Protein Ligases/metabolism, Ubiquitination
- in
- Journal of Cellular and Molecular Medicine
- volume
- 24
- issue
- 2
- pages
- 1980 - 1992
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:85076747769
- pmid:31845480
- ISSN
- 1582-1838
- DOI
- 10.1111/jcmm.14895
- language
- English
- LU publication?
- no
- additional info
- © 2019 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
- id
- 2d21020e-7f7b-4d7e-80dd-faadde336485
- date added to LUP
- 2023-05-11 10:08:48
- date last changed
- 2024-06-15 02:49:06
@article{2d21020e-7f7b-4d7e-80dd-faadde336485, abstract = {{<p>WW domain binding protein 1-like (WBP1L), also known as outcome predictor of acute leukaemia 1 (OPAL1), is a transmembrane adaptor protein, expression of which correlates with ETV6-RUNX1 (t(12;21)(p13;q22)) translocation and favourable prognosis in childhood leukaemia. It has a broad expression pattern in haematopoietic and in non-haematopoietic cells. However, its physiological function has been unknown. Here, we show that WBP1L negatively regulates signalling through a critical chemokine receptor CXCR4 in multiple leucocyte subsets and cell lines. We also show that WBP1L interacts with NEDD4-family ubiquitin ligases and regulates CXCR4 ubiquitination and expression. Moreover, analysis of Wbp1l-deficient mice revealed alterations in B cell development and enhanced efficiency of bone marrow cell transplantation. Collectively, our data show that WBP1L is a novel regulator of CXCR4 signalling and haematopoiesis.</p>}}, author = {{Borna, Simon and Drobek, Ales and Kralova, Jarmila and Glatzova, Daniela and Splichalova, Iva and Fabisik, Matej and Pokorna, Jana and Skopcova, Tereza and Angelisova, Pavla and Kanderova, Veronika and Starkova, Julia and Stanek, Petr and Matveichuk, Orest V and Pavliuchenko, Nataliia and Kwiatkowska, Katarzyna and Protty, Majd B and Tomlinson, Michael G and Alberich-Jorda, Meritxell and Korinek, Vladimir and Brdicka, Tomas}}, issn = {{1582-1838}}, keywords = {{Adaptor Proteins, Signal Transducing/metabolism; Animals; Germ Cells/metabolism; Glycoproteins/metabolism; HEK293 Cells; Hematopoiesis; Hematopoietic Stem Cells/metabolism; Homeostasis; Humans; Lipoylation; Membrane Proteins/genetics; Mice, Inbred C57BL; Protein Binding; RNA, Small Interfering/metabolism; Receptors, CXCR4/metabolism; Signal Transduction; Ubiquitin-Protein Ligases/metabolism; Ubiquitination}}, language = {{eng}}, number = {{2}}, pages = {{1980--1992}}, publisher = {{Wiley-Blackwell}}, series = {{Journal of Cellular and Molecular Medicine}}, title = {{Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis}}, url = {{http://dx.doi.org/10.1111/jcmm.14895}}, doi = {{10.1111/jcmm.14895}}, volume = {{24}}, year = {{2020}}, }