PCR for the diagnosis of enteroviral meningitis
(1994) In Scandinavian Journal of Infectious Diseases 26(3). p.249-254- Abstract
- A 2-step 'semi-nested' enterovirus PCR was developed and applied to CSF and serum specimens from 27 consecutive patients with aseptic meningitis. CSF and sera from 8 patients with non-enteroviral diagnoses were included as negative clinical controls. Enterovirus RNA was detected in CSF by PCR in 15 of the patients with aseptic meningitis, compared with 6 by virus culture. Acute-phase sera proved positive for enterovirus RNA in 11 patients, thus increasing the number of PCR-positive patients to 18. Convalescent-phase sera were all negative by PCR. The correlation of a positive or negative PCR result in CSF and/or serum versus combined conventional virology (serology and isolation from 1-3 sites, i.e. CSF, stool and throat) was 78%. All... (More)
- A 2-step 'semi-nested' enterovirus PCR was developed and applied to CSF and serum specimens from 27 consecutive patients with aseptic meningitis. CSF and sera from 8 patients with non-enteroviral diagnoses were included as negative clinical controls. Enterovirus RNA was detected in CSF by PCR in 15 of the patients with aseptic meningitis, compared with 6 by virus culture. Acute-phase sera proved positive for enterovirus RNA in 11 patients, thus increasing the number of PCR-positive patients to 18. Convalescent-phase sera were all negative by PCR. The correlation of a positive or negative PCR result in CSF and/or serum versus combined conventional virology (serology and isolation from 1-3 sites, i.e. CSF, stool and throat) was 78%. All negative controls were negative by PCR. PCR is a reliable and sensitive diagnostic tool for the detection of enteroviral infections. Both CSF and acute-phase serum should be considered for testing. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107756
- author
- Thorén, Anders and Widell, Anders LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Infectious Diseases
- volume
- 26
- issue
- 3
- pages
- 249 - 254
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:7939423
- scopus:0028270958
- ISSN
- 1651-1980
- DOI
- 10.3109/00365549409011792
- language
- English
- LU publication?
- yes
- id
- 2d52ef24-eff1-43f6-bfcf-c9296457a280 (old id 1107756)
- date added to LUP
- 2016-04-01 15:55:45
- date last changed
- 2021-01-03 08:51:11
@article{2d52ef24-eff1-43f6-bfcf-c9296457a280,
abstract = {{A 2-step 'semi-nested' enterovirus PCR was developed and applied to CSF and serum specimens from 27 consecutive patients with aseptic meningitis. CSF and sera from 8 patients with non-enteroviral diagnoses were included as negative clinical controls. Enterovirus RNA was detected in CSF by PCR in 15 of the patients with aseptic meningitis, compared with 6 by virus culture. Acute-phase sera proved positive for enterovirus RNA in 11 patients, thus increasing the number of PCR-positive patients to 18. Convalescent-phase sera were all negative by PCR. The correlation of a positive or negative PCR result in CSF and/or serum versus combined conventional virology (serology and isolation from 1-3 sites, i.e. CSF, stool and throat) was 78%. All negative controls were negative by PCR. PCR is a reliable and sensitive diagnostic tool for the detection of enteroviral infections. Both CSF and acute-phase serum should be considered for testing.}},
author = {{Thorén, Anders and Widell, Anders}},
issn = {{1651-1980}},
language = {{eng}},
number = {{3}},
pages = {{249--254}},
publisher = {{Informa Healthcare}},
series = {{Scandinavian Journal of Infectious Diseases}},
title = {{PCR for the diagnosis of enteroviral meningitis}},
url = {{http://dx.doi.org/10.3109/00365549409011792}},
doi = {{10.3109/00365549409011792}},
volume = {{26}},
year = {{1994}},
}