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Chitosan does not inhibit enzymatic action of human pancreatic lipase in Langmuir monolayers of 1,2-didecanoyl-glycerol (DDG)

Souza, Adriano L. ; Pavinatto, Felippe J. ; Caseli, Luciano ; Volpati, Diogo LU ; Miranda, Paulo B. and Oliveira, Osvaldo N. (2014) In Colloids and Surfaces B: Biointerfaces 123. p.870-877
Abstract

In this study, we tested the hypothesis according to which chitosan reduces lipid digestion by blocking the access of lipases to ingested fat. Because lipase action takes place mostly at interfaces, we produced Langmuir films of 1,2-didecanoyl-glycerol (DDG), which is the substrate for human pancreatic lipase (HPL). The experimental assays were carried out in acidic medium, at pH 3.0, to ensure that chitosan is completely soluble. Chitosan was found to affect strongly the surface activity of HPL that forms a Gibbs monolayer at the air/water interface, but did not inhibit the enzymatic action of HPL toward the DDG monolayer. The latter was observed using two surface-specific spectroscopic techniques, namely polarization-modulated... (More)

In this study, we tested the hypothesis according to which chitosan reduces lipid digestion by blocking the access of lipases to ingested fat. Because lipase action takes place mostly at interfaces, we produced Langmuir films of 1,2-didecanoyl-glycerol (DDG), which is the substrate for human pancreatic lipase (HPL). The experimental assays were carried out in acidic medium, at pH 3.0, to ensure that chitosan is completely soluble. Chitosan was found to affect strongly the surface activity of HPL that forms a Gibbs monolayer at the air/water interface, but did not inhibit the enzymatic action of HPL toward the DDG monolayer. The latter was observed using two surface-specific spectroscopic techniques, namely polarization-modulated infrared reflection-absorption and sum-frequency generation (SFG). The extension of DDG hydrolysis calculated using SFG spectroscopy was 33% in the absence of chitosan, and ranged from 29 to 50% in the presence of chitosan at concentrations of 0.20gL-1 and 0.30gL-1, respectively. Therefore, fat "protection" by chitosan is unlikely to be an important factor in fat reduction.

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publishing date
type
Contribution to journal
publication status
published
keywords
Chitosan, Fat reduction, Human pancreatic lipase, Langmuir monolayers, Sum-frequency generation spectroscopy
in
Colloids and Surfaces B: Biointerfaces
volume
123
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:84915823197
ISSN
0927-7765
DOI
10.1016/j.colsurfb.2014.10.040
language
English
LU publication?
no
id
2d8de800-7ec3-491d-a094-511af53faf10
date added to LUP
2019-05-17 14:33:34
date last changed
2020-05-20 04:42:10
@article{2d8de800-7ec3-491d-a094-511af53faf10,
  abstract     = {<p>In this study, we tested the hypothesis according to which chitosan reduces lipid digestion by blocking the access of lipases to ingested fat. Because lipase action takes place mostly at interfaces, we produced Langmuir films of 1,2-didecanoyl-glycerol (DDG), which is the substrate for human pancreatic lipase (HPL). The experimental assays were carried out in acidic medium, at pH 3.0, to ensure that chitosan is completely soluble. Chitosan was found to affect strongly the surface activity of HPL that forms a Gibbs monolayer at the air/water interface, but did not inhibit the enzymatic action of HPL toward the DDG monolayer. The latter was observed using two surface-specific spectroscopic techniques, namely polarization-modulated infrared reflection-absorption and sum-frequency generation (SFG). The extension of DDG hydrolysis calculated using SFG spectroscopy was 33% in the absence of chitosan, and ranged from 29 to 50% in the presence of chitosan at concentrations of 0.20gL<sup>-1</sup> and 0.30gL<sup>-1</sup>, respectively. Therefore, fat "protection" by chitosan is unlikely to be an important factor in fat reduction.</p>},
  author       = {Souza, Adriano L. and Pavinatto, Felippe J. and Caseli, Luciano and Volpati, Diogo and Miranda, Paulo B. and Oliveira, Osvaldo N.},
  issn         = {0927-7765},
  language     = {eng},
  month        = {11},
  pages        = {870--877},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Chitosan does not inhibit enzymatic action of human pancreatic lipase in Langmuir monolayers of 1,2-didecanoyl-glycerol (DDG)},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2014.10.040},
  doi          = {10.1016/j.colsurfb.2014.10.040},
  volume       = {123},
  year         = {2014},
}