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Lack of protection by the N-methyl-D-asparate receptor blocker dizocilpine (MK-801) after transient severe cerebral ischemia in the rat

Nellgard, B. LU ; Gustafson, I. and Wieloch, T. LU (1991) In Anesthesiology 75(2). p.279-287
Abstract

Glutamate is an important factor in the mechanisms of neuronal damage following cerebral ischemia. Blockade of one type of glutamate receptor, the N-methyl-D-aspartate (NMDA) receptor, decreases brain infarct size in experimental models of permanent focal ischemia, but protection in models of transient reversible ischemia is ambiguous. We investigated the effect of the noncompetitive NMDA receptor antagonist dizocipiline (MK-801) on neuronal damage in the CA1 region of the rat hippocampus, using two models of reversible cerebral ischemia: 10 or 15 min of bilateral common carotid occlusion combined with hypotension, or 6-8.5 min of cardiac arrest. Histopathologic evaluation of neuronal damage was performed 7 days after the ischemic... (More)

Glutamate is an important factor in the mechanisms of neuronal damage following cerebral ischemia. Blockade of one type of glutamate receptor, the N-methyl-D-aspartate (NMDA) receptor, decreases brain infarct size in experimental models of permanent focal ischemia, but protection in models of transient reversible ischemia is ambiguous. We investigated the effect of the noncompetitive NMDA receptor antagonist dizocipiline (MK-801) on neuronal damage in the CA1 region of the rat hippocampus, using two models of reversible cerebral ischemia: 10 or 15 min of bilateral common carotid occlusion combined with hypotension, or 6-8.5 min of cardiac arrest. Histopathologic evaluation of neuronal damage was performed 7 days after the ischemic insults. Thirteen groups of rats (a total of 129 animals) were treated with saline or dizocilpine in single or multiple doses ranging from 0.1 to 5 mg·kg-1, given intravenously or intraperitoneally prior to and/or after the ischemic insult. In none of the dizocilpine-treated groups could neuronal protection be demonstrated in the CA1 region of the septal as well as dorsotemporal hippocampus, compared to a corresponding saline-treated group. We conclude that systemically administered noncompetitive NMDA receptor antagonists do not provide a marked protection against neuronal damage after a transient period of severe forebrain ischemia.

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author
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organization
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publication status
published
subject
keywords
Antagonists, glutamate: dizocilpine, Brain, ischemia: cell death; protection, Brain: hippocampus, Receptors: glutamate
in
Anesthesiology
volume
75
issue
2
pages
9 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:1859015
  • scopus:0025931596
ISSN
0003-3022
DOI
10.1097/00000542-199108000-00016
language
English
LU publication?
yes
id
2dbf161c-a6c1-422a-a727-31433056960c
date added to LUP
2019-06-13 16:26:34
date last changed
2020-03-15 06:00:42
@article{2dbf161c-a6c1-422a-a727-31433056960c,
  abstract     = {<p>Glutamate is an important factor in the mechanisms of neuronal damage following cerebral ischemia. Blockade of one type of glutamate receptor, the N-methyl-D-aspartate (NMDA) receptor, decreases brain infarct size in experimental models of permanent focal ischemia, but protection in models of transient reversible ischemia is ambiguous. We investigated the effect of the noncompetitive NMDA receptor antagonist dizocipiline (MK-801) on neuronal damage in the CA1 region of the rat hippocampus, using two models of reversible cerebral ischemia: 10 or 15 min of bilateral common carotid occlusion combined with hypotension, or 6-8.5 min of cardiac arrest. Histopathologic evaluation of neuronal damage was performed 7 days after the ischemic insults. Thirteen groups of rats (a total of 129 animals) were treated with saline or dizocilpine in single or multiple doses ranging from 0.1 to 5 mg·kg<sup>-1</sup>, given intravenously or intraperitoneally prior to and/or after the ischemic insult. In none of the dizocilpine-treated groups could neuronal protection be demonstrated in the CA1 region of the septal as well as dorsotemporal hippocampus, compared to a corresponding saline-treated group. We conclude that systemically administered noncompetitive NMDA receptor antagonists do not provide a marked protection against neuronal damage after a transient period of severe forebrain ischemia.</p>},
  author       = {Nellgard, B. and Gustafson, I. and Wieloch, T.},
  issn         = {0003-3022},
  language     = {eng},
  number       = {2},
  pages        = {279--287},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Anesthesiology},
  title        = {Lack of protection by the N-methyl-D-asparate receptor blocker dizocilpine (MK-801) after transient severe cerebral ischemia in the rat},
  url          = {http://dx.doi.org/10.1097/00000542-199108000-00016},
  doi          = {10.1097/00000542-199108000-00016},
  volume       = {75},
  year         = {1991},
}