High-throughput identification of genes promoting neuron formation and lineage choice in mouse embryonic stem cells
Falk, Anna LU ; Karlsson, Tobias E LU
; Kurdija, Sanja
; Frisén, Jonas
and Zupicich, Joel
(2007)
In Stem Cells
25(6).
p.45-1539
- Abstract
The potential of embryonic stem cells to differentiate to all cell types makes them an attractive model for development and a potential source of cells for transplantation therapies. Candidate approaches have identified individual genes and proteins that promote the differentiation of embryonic stem cells to desired fates. Here, we describe a rapid large-scale screening strategy for the identification of genes that influence the pluripotency and differentiation of embryonic stem cells to specific fates, and we use this approach to identify genes that induce neuron formation. The power of the strategy is validated by the fact that, of the 15 genes that resulted in the largest increase in neuron number, 8 have previously been implicated... (More)
The potential of embryonic stem cells to differentiate to all cell types makes them an attractive model for development and a potential source of cells for transplantation therapies. Candidate approaches have identified individual genes and proteins that promote the differentiation of embryonic stem cells to desired fates. Here, we describe a rapid large-scale screening strategy for the identification of genes that influence the pluripotency and differentiation of embryonic stem cells to specific fates, and we use this approach to identify genes that induce neuron formation. The power of the strategy is validated by the fact that, of the 15 genes that resulted in the largest increase in neuron number, 8 have previously been implicated in neuronal differentiation or survival, whereas 7 represent novel genes or known genes not previously implicated in neuronal development. This is a simple, fast, and generally applicable strategy for the identification of genes promoting the formation of any specific cell type from embryonic stem cells. Disclosure of potential conflicts of interest is found at the end of this article.
(Less)
- author
- Falk, Anna
LU
; Karlsson, Tobias E
LU
; Kurdija, Sanja
; Frisén, Jonas
and Zupicich, Joel
- publishing date
- 2007-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Cell Differentiation/genetics, Cell Lineage/genetics, Cells, Cultured, Embryonic Stem Cells/cytology, Gene Expression Profiling/methods, Genes, Developmental, Mice, Models, Biological, Neurons/cytology, Organ Specificity/genetics, Transfection/methods
- in
- Stem Cells
- volume
- 25
- issue
- 6
- pages
- 7 pages
- publisher
- Oxford University Press
- external identifiers
-
- scopus:34547122872
- pmid:17379767
- ISSN
- 1066-5099
- DOI
- 10.1634/stemcells.2006-0485
- language
- English
- LU publication?
- no
- id
- 2e074a9b-3835-440b-85d4-9cdc92dd8689
- date added to LUP
- 2021-08-10 13:55:29
- date last changed
- 2024-03-08 16:31:54
@article{2e074a9b-3835-440b-85d4-9cdc92dd8689,
abstract = {{<p>The potential of embryonic stem cells to differentiate to all cell types makes them an attractive model for development and a potential source of cells for transplantation therapies. Candidate approaches have identified individual genes and proteins that promote the differentiation of embryonic stem cells to desired fates. Here, we describe a rapid large-scale screening strategy for the identification of genes that influence the pluripotency and differentiation of embryonic stem cells to specific fates, and we use this approach to identify genes that induce neuron formation. The power of the strategy is validated by the fact that, of the 15 genes that resulted in the largest increase in neuron number, 8 have previously been implicated in neuronal differentiation or survival, whereas 7 represent novel genes or known genes not previously implicated in neuronal development. This is a simple, fast, and generally applicable strategy for the identification of genes promoting the formation of any specific cell type from embryonic stem cells. Disclosure of potential conflicts of interest is found at the end of this article.</p>}},
author = {{Falk, Anna and Karlsson, Tobias E and Kurdija, Sanja and Frisén, Jonas and Zupicich, Joel}},
issn = {{1066-5099}},
keywords = {{Animals; Cell Differentiation/genetics; Cell Lineage/genetics; Cells, Cultured; Embryonic Stem Cells/cytology; Gene Expression Profiling/methods; Genes, Developmental; Mice; Models, Biological; Neurons/cytology; Organ Specificity/genetics; Transfection/methods}},
language = {{eng}},
number = {{6}},
pages = {{45--1539}},
publisher = {{Oxford University Press}},
series = {{Stem Cells}},
title = {{High-throughput identification of genes promoting neuron formation and lineage choice in mouse embryonic stem cells}},
url = {{http://dx.doi.org/10.1634/stemcells.2006-0485}},
doi = {{10.1634/stemcells.2006-0485}},
volume = {{25}},
year = {{2007}},
}