Adipose Triglyceride Lipase Deficiency Attenuates In Vitro Thrombus Formation without Affecting Platelet Activation and Bleeding In Vivo
Goeritzer, Madeleine ; Schlager, Stefanie ; Kuentzel, Katharina B LU
; Vujić, Nemanja
; Korbelius, Melanie
; Rainer, Silvia
; Kolb, Dagmar
; Mussbacher, Marion
; Salzmann, Manuel
and Schrottmaier, Waltraud C
, et al.
(2022)
In Cells
11(5).
p.1-18
- Abstract
According to genome-wide RNA sequencing data from human and mouse platelets, adipose triglyceride lipase (ATGL), the main lipase catalyzing triglyceride (TG) hydrolysis in cytosolic lipid droplets (LD) at neutral pH, is expressed in platelets. Currently, it is elusive to whether common lipolytic enzymes are involved in the degradation of TG in platelets. Since the consequences of ATGL deficiency in platelets are unknown, we used whole-body and platelet-specific (plat)Atgl-deficient (-/-) mice to investigate the loss of ATGL on platelet function. Our results showed that platelets accumulate only a few LD due to lack of ATGL. Stimulation with platelet-activating agonists resulted in comparable platelet activation in Atgl-/-, platAtgl-/-,... (More)
According to genome-wide RNA sequencing data from human and mouse platelets, adipose triglyceride lipase (ATGL), the main lipase catalyzing triglyceride (TG) hydrolysis in cytosolic lipid droplets (LD) at neutral pH, is expressed in platelets. Currently, it is elusive to whether common lipolytic enzymes are involved in the degradation of TG in platelets. Since the consequences of ATGL deficiency in platelets are unknown, we used whole-body and platelet-specific (plat)Atgl-deficient (-/-) mice to investigate the loss of ATGL on platelet function. Our results showed that platelets accumulate only a few LD due to lack of ATGL. Stimulation with platelet-activating agonists resulted in comparable platelet activation in Atgl-/-, platAtgl-/-, and wild-type mice. Measurement of mitochondrial respiration revealed a decreased oxygen consumption rate in platelets from Atgl-/- but not from platAtgl-/- mice. Of note, global loss of ATGL was associated with an anti-thrombogenic phenotype, which was evident by reduced thrombus formation in collagen-coated channels in vitro despite unchanged bleeding and occlusion times in vivo. We conclude that genetic deletion of ATGL affects collagen-induced thrombosis without pathological bleeding and platelet activation.
(Less)
- author
- Goeritzer, Madeleine
; Schlager, Stefanie
; Kuentzel, Katharina B
LU
; Vujić, Nemanja
; Korbelius, Melanie
; Rainer, Silvia
; Kolb, Dagmar
; Mussbacher, Marion
; Salzmann, Manuel
and Schrottmaier, Waltraud C
, et al. (More)
- Goeritzer, Madeleine
; Schlager, Stefanie
; Kuentzel, Katharina B
LU
; Vujić, Nemanja
; Korbelius, Melanie
; Rainer, Silvia
; Kolb, Dagmar
; Mussbacher, Marion
; Salzmann, Manuel
; Schrottmaier, Waltraud C
; Assinger, Alice
; Schlagenhauf, Axel
; Madreiter-Sokolowski, Corina T
; Blass, Sandra
; Eichmann, Thomas O
; Graier, Wolfgang F
and Kratky, Dagmar
(Less)
- publishing date
- 2022-03-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Acyltransferases/metabolism, Animals, Lipase/metabolism, Mice, Mice, Knockout, Platelet Activation, Thrombosis, Triglycerides/metabolism
- in
- Cells
- volume
- 11
- issue
- 5
- article number
- 850
- pages
- 1 - 18
- publisher
- MDPI AG
- external identifiers
-
- scopus:85125997113
- pmid:35269472
- ISSN
- 2073-4409
- DOI
- 10.3390/cells11050850
- language
- English
- LU publication?
- no
- id
- 2e5e0a0d-e716-46af-833f-67d38c92249e
- date added to LUP
- 2022-10-10 16:29:24
- date last changed
- 2024-06-27 15:58:51
@article{2e5e0a0d-e716-46af-833f-67d38c92249e,
abstract = {{<p>According to genome-wide RNA sequencing data from human and mouse platelets, adipose triglyceride lipase (ATGL), the main lipase catalyzing triglyceride (TG) hydrolysis in cytosolic lipid droplets (LD) at neutral pH, is expressed in platelets. Currently, it is elusive to whether common lipolytic enzymes are involved in the degradation of TG in platelets. Since the consequences of ATGL deficiency in platelets are unknown, we used whole-body and platelet-specific (plat)Atgl-deficient (-/-) mice to investigate the loss of ATGL on platelet function. Our results showed that platelets accumulate only a few LD due to lack of ATGL. Stimulation with platelet-activating agonists resulted in comparable platelet activation in Atgl-/-, platAtgl-/-, and wild-type mice. Measurement of mitochondrial respiration revealed a decreased oxygen consumption rate in platelets from Atgl-/- but not from platAtgl-/- mice. Of note, global loss of ATGL was associated with an anti-thrombogenic phenotype, which was evident by reduced thrombus formation in collagen-coated channels in vitro despite unchanged bleeding and occlusion times in vivo. We conclude that genetic deletion of ATGL affects collagen-induced thrombosis without pathological bleeding and platelet activation.</p>}},
author = {{Goeritzer, Madeleine and Schlager, Stefanie and Kuentzel, Katharina B and Vujić, Nemanja and Korbelius, Melanie and Rainer, Silvia and Kolb, Dagmar and Mussbacher, Marion and Salzmann, Manuel and Schrottmaier, Waltraud C and Assinger, Alice and Schlagenhauf, Axel and Madreiter-Sokolowski, Corina T and Blass, Sandra and Eichmann, Thomas O and Graier, Wolfgang F and Kratky, Dagmar}},
issn = {{2073-4409}},
keywords = {{Acyltransferases/metabolism; Animals; Lipase/metabolism; Mice; Mice, Knockout; Platelet Activation; Thrombosis; Triglycerides/metabolism}},
language = {{eng}},
month = {{03}},
number = {{5}},
pages = {{1--18}},
publisher = {{MDPI AG}},
series = {{Cells}},
title = {{Adipose Triglyceride Lipase Deficiency Attenuates In Vitro Thrombus Formation without Affecting Platelet Activation and Bleeding In Vivo}},
url = {{http://dx.doi.org/10.3390/cells11050850}},
doi = {{10.3390/cells11050850}},
volume = {{11}},
year = {{2022}},
}