Reduced expression of C5a receptors on neutrophils from cord blood
(1998) In Archives of disease in childhood. Fetal and neonatal edition 78(2). p.32-129- Abstract
AIM: To describe further functional deficiencies of neonatal neutrophils by measuring the expression of C5a receptors.
METHODS: C5a uptake was measured using flow cytometry with fluorescein isothiocynate labelled recombinant C5a. The response of neutrophils to stimulation with C5a and fMLP was tested by measuring migration and exocytosis of myeloperoxidase and lactoferrin.
RESULTS: C5a mean fluorescence on neutrophils from neonates was significantly lower (22.4 (SD 3.5)) than in adult controls (31.5 (3.1)). Neutrophils from neonates migrated poorly towards both C5a and fMLP compared with those from adult controls. Exocytosis of myeloperoxidase, but not lactoferrin from neonatal neutrophils stimulated with C5a, was... (More)
AIM: To describe further functional deficiencies of neonatal neutrophils by measuring the expression of C5a receptors.
METHODS: C5a uptake was measured using flow cytometry with fluorescein isothiocynate labelled recombinant C5a. The response of neutrophils to stimulation with C5a and fMLP was tested by measuring migration and exocytosis of myeloperoxidase and lactoferrin.
RESULTS: C5a mean fluorescence on neutrophils from neonates was significantly lower (22.4 (SD 3.5)) than in adult controls (31.5 (3.1)). Neutrophils from neonates migrated poorly towards both C5a and fMLP compared with those from adult controls. Exocytosis of myeloperoxidase, but not lactoferrin from neonatal neutrophils stimulated with C5a, was significantly lower than in adult controls. fMLP stimulation, on the other hand, resulted in significantly higher exocytosis in neonates.
CONCLUSION: The lower expression of C5a receptors on neutrophils from neonates could be related to reduced C5a mediated exocytosis of myeloperoxidase.
(Less)
- author
- Nybo, M ; Sørensen, O LU ; Leslie, R and Wang, P
- publishing date
- 1998-03
- type
- Contribution to journal
- publication status
- published
- keywords
- Antigens, CD/metabolism, Cells, Cultured, Complement C5a/metabolism, Fetal Blood/immunology, Flow Cytometry, Humans, Infant, Newborn, N-Formylmethionine Leucyl-Phenylalanine/metabolism, Neutrophils/immunology, Peroxidase/metabolism, Receptor, Anaphylatoxin C5a, Receptors, Complement/metabolism
- in
- Archives of disease in childhood. Fetal and neonatal edition
- volume
- 78
- issue
- 2
- pages
- 32 - 129
- publisher
- BMJ Publishing Group
- external identifiers
-
- pmid:9577284
- scopus:0031896436
- ISSN
- 1359-2998
- DOI
- 10.1136/fn.78.2.f129
- language
- English
- LU publication?
- no
- id
- 2e6f5a02-7845-48eb-8ef2-dbe66d173206
- date added to LUP
- 2020-11-16 13:17:37
- date last changed
- 2024-01-02 22:06:05
@article{2e6f5a02-7845-48eb-8ef2-dbe66d173206,
abstract = {{<p>AIM: To describe further functional deficiencies of neonatal neutrophils by measuring the expression of C5a receptors.</p><p>METHODS: C5a uptake was measured using flow cytometry with fluorescein isothiocynate labelled recombinant C5a. The response of neutrophils to stimulation with C5a and fMLP was tested by measuring migration and exocytosis of myeloperoxidase and lactoferrin.</p><p>RESULTS: C5a mean fluorescence on neutrophils from neonates was significantly lower (22.4 (SD 3.5)) than in adult controls (31.5 (3.1)). Neutrophils from neonates migrated poorly towards both C5a and fMLP compared with those from adult controls. Exocytosis of myeloperoxidase, but not lactoferrin from neonatal neutrophils stimulated with C5a, was significantly lower than in adult controls. fMLP stimulation, on the other hand, resulted in significantly higher exocytosis in neonates.</p><p>CONCLUSION: The lower expression of C5a receptors on neutrophils from neonates could be related to reduced C5a mediated exocytosis of myeloperoxidase.</p>}},
author = {{Nybo, M and Sørensen, O and Leslie, R and Wang, P}},
issn = {{1359-2998}},
keywords = {{Antigens, CD/metabolism; Cells, Cultured; Complement C5a/metabolism; Fetal Blood/immunology; Flow Cytometry; Humans; Infant, Newborn; N-Formylmethionine Leucyl-Phenylalanine/metabolism; Neutrophils/immunology; Peroxidase/metabolism; Receptor, Anaphylatoxin C5a; Receptors, Complement/metabolism}},
language = {{eng}},
number = {{2}},
pages = {{32--129}},
publisher = {{BMJ Publishing Group}},
series = {{Archives of disease in childhood. Fetal and neonatal edition}},
title = {{Reduced expression of C5a receptors on neutrophils from cord blood}},
url = {{http://dx.doi.org/10.1136/fn.78.2.f129}},
doi = {{10.1136/fn.78.2.f129}},
volume = {{78}},
year = {{1998}},
}